ID A6UEI6_SINMW Unreviewed; 1189 AA.
AC A6UEI6;
DT 21-AUG-2007, integrated into UniProtKB/TrEMBL.
DT 21-AUG-2007, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN OrderedLocusNames=Smed_3242 {ECO:0000313|EMBL:ABR62066.1};
OS Sinorhizobium medicae (strain WSM419) (Ensifer medicae).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=366394 {ECO:0000313|EMBL:ABR62066.1, ECO:0000313|Proteomes:UP000001108};
RN [1] {ECO:0000313|Proteomes:UP000001108}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WSM419 {ECO:0000313|Proteomes:UP000001108};
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G.,
RA Richardson P.;
RT "Complete sequence of Sinorhizobium medicae WSM419 chromosome.";
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ABR62066.1, ECO:0000313|Proteomes:UP000001108}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WSM419 {ECO:0000313|EMBL:ABR62066.1,
RC ECO:0000313|Proteomes:UP000001108};
RX PubMed=21304680; DOI=10.4056/sigs.43526;
RA Reeve W., Chain P., O'Hara G., Ardley J., Nandesena K., Brau L., Tiwari R.,
RA Malfatti S., Kiss H., Lapidus A., Copeland A., Nolan M., Land M.,
RA Hauser L., Chang Y.J., Ivanova N., Mavromatis K., Markowitz V.,
RA Kyrpides N., Gollagher M., Yates R., Dilworth M., Howieson J.;
RT "Complete genome sequence of the Medicago microsymbiont Ensifer
RT (Sinorhizobium) medicae strain WSM419.";
RL Stand. Genomic Sci. 2:77-86(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR EMBL; CP000738; ABR62066.1; -; Genomic_DNA.
DR RefSeq; WP_012067447.1; NC_009636.1.
DR RefSeq; YP_001328901.1; NC_009636.1.
DR AlphaFoldDB; A6UEI6; -.
DR STRING; 366394.Smed_3242; -.
DR GeneID; 61610824; -.
DR KEGG; smd:Smed_3242; -.
DR PATRIC; fig|366394.8.peg.6480; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_0_0_5; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000001108; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014151; DNA_helicase_AddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR02784; addA_alphas; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 17..503
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 528..811
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 950..980
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 38..45
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1189 AA; 130198 MW; 5F676EFF974A29B3 CRC64;
MRSDATGPEE RTPEGWLDWT TERQALASDP ARSAWVSANA GSGKTHVLTQ RVIRLLLAGC
RPSAILCLTY TKAAASEMSN RVFEKLAEWA TLDDAALEKR IEAIEGKRPA TGKIQEARRL
FARALETPGG LKIQTIHAFC EALLHQFPLE ANVAGHFSVL DDRAAAVLLA DARRALLTAT
AAANDRELAA AFATVLELAD DTGLEKLLAA IVANRAQIQA FLDRVSGRGG LDTHLRAALD
LNPGDTPEAI MAAAWPLAGL NGAALVDYIG LASRLGGTKP TAIAEGFRTV RAIGDPAARY
DRLLETFLNG GGKPKADSAF LNAAMRREAP HLESLIEEAR SHLLACVDRL SIVRMYEATR
AALVLAERLN RDYESLKKAR SQLDFEDLIN RTAALLARSD VGAWVHYKLD QGIDHILVDE
AQDTSPAQWT IIQSLAADFF AGDTARADDR TIFAVGDEKQ SIYSFQGARP ERFSRERTLT
ERRVRAGNKR FSPIRLQLSF RSTVDVLSAV DTVFANIGNA RGLSARDEAI VHASNRIGQP
GAVDLWEVIA PEPAASDEDW TAPFDATPER APVNILARRI AAVLEDWIGK ETVIEKGVRR
AMRPGDVIVL VRKRDAFVNA LMRALKRRGN IPVAGADRLV LTSHIAVQDL IALGRFVLLP
EDDLSLAALF KSPLVNLCED DVFELAARRN EGESLWHRLQ QAGVEETSRY NATVRKLAAF
STLSRELSPH DFYARILGAH GGRRAFLARL GSEVSDILDE FLTFALDHER SGLPGLQAFI
STLEIEAPTV KREQDKERDE VRVMTVHAAK GLEAPVVFLV DGGGEAFVRQ QVSDLRFLEK
PQADQTLLTV PVWRAPGSAP NSLIADDNER LKMLAEEEYR RLLYVGMTRA ADRLIVCGYR
GQRQNVDTWH SMVQGTLAQD LMGRATPRLF RAAGEEWQGL AWRETHVPRD LPAADGASGE
PEQAKAGLPR ALFTAPPPPR RLPRPLAPSG TAIAIDEPDG SSIVGSALFT GKNAPKFSML
RGAILHRLLQ VLPSIDAVER TAAAERYLAR SVPRWPEAER LALAATVMDV LGHAELQPLF
GEHSRAEVSV MGTLKLGPRE FAVSGRIDRL AVSGDTVTIA DYKTNREIPA SAEDIAPVYR
NQLAIYRELL KPLYPGKRFR CVLIFTEGPA IRVLSEAMLD RSLEALATK
//
