ID A7A5N6_BIFAD Unreviewed; 702 AA.
AC A7A5N6;
DT 11-SEP-2007, integrated into UniProtKB/TrEMBL.
DT 11-SEP-2007, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=transketolase {ECO:0000256|ARBA:ARBA00013152};
DE EC=2.2.1.1 {ECO:0000256|ARBA:ARBA00013152};
GN Name=tkt {ECO:0000313|EMBL:EDN82874.1};
GN ORFNames=BIFADO_01156 {ECO:0000313|EMBL:EDN82874.1};
OS Bifidobacterium adolescentis L2-32.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=411481 {ECO:0000313|EMBL:EDN82874.1, ECO:0000313|Proteomes:UP000003773};
RN [1] {ECO:0000313|EMBL:EDN82874.1, ECO:0000313|Proteomes:UP000003773}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=L2-32 {ECO:0000313|EMBL:EDN82874.1,
RC ECO:0000313|Proteomes:UP000003773};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Thiruvilangam P., Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EDN82874.1, ECO:0000313|Proteomes:UP000003773}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=L2-32 {ECO:0000313|EMBL:EDN82874.1,
RC ECO:0000313|Proteomes:UP000003773};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Bifidobacterium adolescentis (L2-32).";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate =
CC aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate;
CC Xref=Rhea:RHEA:10508, ChEBI:CHEBI:57483, ChEBI:CHEBI:57737,
CC ChEBI:CHEBI:58273, ChEBI:CHEBI:59776; EC=2.2.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001027};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC Evidence={ECO:0000256|ARBA:ARBA00001964};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: Belongs to the transketolase family.
CC {ECO:0000256|ARBA:ARBA00007131}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDN82874.1}.
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DR EMBL; AAXD02000028; EDN82874.1; -; Genomic_DNA.
DR RefSeq; WP_003809218.1; NZ_DS264455.1.
DR AlphaFoldDB; A7A5N6; -.
DR GeneID; 56675072; -.
DR HOGENOM; CLU_009227_0_0_11; -.
DR Proteomes; UP000003773; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProt.
DR GO; GO:0004802; F:transketolase activity; IEA:UniProtKB-EC.
DR CDD; cd07033; TPP_PYR_DXS_TK_like; 1.
DR CDD; cd02012; TPP_TK; 1.
DR Gene3D; 3.40.50.920; -; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR009014; Transketo_C/PFOR_II.
DR InterPro; IPR005475; Transketolase-like_Pyr-bd.
DR InterPro; IPR005478; Transketolase_bac-like.
DR InterPro; IPR020826; Transketolase_BS.
DR InterPro; IPR033248; Transketolase_C.
DR InterPro; IPR033247; Transketolase_fam.
DR InterPro; IPR005474; Transketolase_N.
DR NCBIfam; TIGR00232; tktlase_bact; 1.
DR PANTHER; PTHR43522; TRANSKETOLASE; 1.
DR PANTHER; PTHR43522:SF2; TRANSKETOLASE 1-RELATED; 1.
DR Pfam; PF02779; Transket_pyr; 1.
DR Pfam; PF02780; Transketolase_C; 1.
DR Pfam; PF00456; Transketolase_N; 1.
DR SMART; SM00861; Transket_pyr; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR SUPFAM; SSF52922; TK C-terminal domain-like; 1.
DR PROSITE; PS00802; TRANSKETOLASE_2; 1.
PE 3: Inferred from homology;
KW Thiamine pyrophosphate {ECO:0000256|ARBA:ARBA00023052};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EDN82874.1}.
FT DOMAIN 374..556
FT /note="Transketolase-like pyrimidine-binding"
FT /evidence="ECO:0000259|SMART:SM00861"
SQ SEQUENCE 702 AA; 76085 MW; 7EDFD1B78F44BBE8 CRC64;
MTEFKETELD ERAIKMAKVL SADAVERAGH GHPGSPVSLA PIAYTLYQHF IKHDPNDPNW
EGRDRFILSG GHASLTQYVQ LYFSGYGLTL DDLKNFRGGA DTRTPGHPEY GLTPGIEMTT
GPLGQGFASA IGFAYGQRFQ RGLLDPEAPE GESPFDHNIW VICGEGDIEE GISGEAASLA
ANQQLGNLTV IFDANRIQIE GDTNLVLAED VLKRFQAYGW YTDEFSFIQP DGSYKEDVEG
LADTIAKARA AAPNQPKLIK VDTLIAWPTP GKTNDPSSHG SKLGAEAVAG LKKLLGYDPE
ESFHVDEEAL AHARKVAERG LEAHKEWDEK YNAWRKANPD KAALYDRIKA GELPEGFDKA
IDDLEATFEV GKNVATRGAS GSTLNAIAAV MPELWGGSAD LGGSNKTDLK GAETFAPAEC
ATKQWPNCSK YGRQLHFGVR EFTMGTITNG ILLGSHTRPY GGTFFMFSDY ERSAVRLAAL
MQIPNLYVWS HDSVAVGEDG PTHQPVEHLA SFRAIPQLEV ARPADAYETA EAYRYFFEKK
NTLPTAMVLT RQGVPVLAET AEKAKDGVKK GAYVLVDTEG TPDVIIMATG SEVQWAVSAA
KTLAGEGIKA RVVSVPCVEW FEEQDAEYKE AVLPAAVKAR VSVEAGVAMP WYKYLGSYGK
PVSIEQFGLQ GDGAQNMIDL GITAEHVVEA AKASIAEVEA AK
//
