ID A7GTF8_BACCN Unreviewed; 3099 AA.
AC A7GTF8;
DT 11-SEP-2007, integrated into UniProtKB/TrEMBL.
DT 11-SEP-2007, sequence version 1.
DT 27-MAR-2024, entry version 108.
DE SubName: Full=Beta-ketoacyl synthase {ECO:0000313|EMBL:ABS23416.1};
GN OrderedLocusNames=Bcer98_3193 {ECO:0000313|EMBL:ABS23416.1};
OS Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=315749 {ECO:0000313|EMBL:ABS23416.1, ECO:0000313|Proteomes:UP000002300};
RN [1] {ECO:0000313|EMBL:ABS23416.1, ECO:0000313|Proteomes:UP000002300}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22905 / CIP 110041 / 391-98 / NVH 391-98
RC {ECO:0000313|Proteomes:UP000002300};
RX PubMed=17434157; DOI=10.1016/j.cbi.2007.03.003;
RA Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C.,
RA Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V.,
RA Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J.,
RA Ehrlich S.D., Sorokin A.;
RT "Extending the Bacillus cereus group genomics to putative food-borne
RT pathogens of different toxicity.";
RL Chem. Biol. Interact. 171:236-249(2008).
CC -!- PATHWAY: Antibiotic biosynthesis; bacillaene biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004789}.
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DR EMBL; CP000764; ABS23416.1; -; Genomic_DNA.
DR STRING; 315749.Bcer98_3193; -.
DR KEGG; bcy:Bcer98_3193; -.
DR eggNOG; COG3321; Bacteria.
DR HOGENOM; CLU_000402_0_0_9; -.
DR OrthoDB; 9765680at2; -.
DR Proteomes; UP000002300; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR CDD; cd08953; KR_2_SDR_x; 1.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 2.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR049490; C883_1060-like_KR_N.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF21394; Beta-ketacyl_N; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF00550; PP-binding; 2.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 1.
DR SMART; SM01294; PKS_PP_betabranch; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 2.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 95..114
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 609..686
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 709..1165
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2134..2208
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2231..2693
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
SQ SEQUENCE 3099 AA; 347353 MW; 13A3CEB16EC52D56 CRC64;
MKRVKREKNC KLSIAIGKNI EGELYKKENI GRLLMEAADE RKDSGITFIQ NDGVEDFLSY
ESLLNRAVRC LGGLQKKGIQ QGQHVMLVLE DSRDFIISFW ACILGGIIPV PLVYPTSLKM
KNSILDKLFV VWNLLEKPVI LSDDHVVNHH EKIESLLAVK GMEFLSISTL YSANEGGEIN
LAPSDTPAFI QFSSGSTSVP KGVILTHRNL LTNIEAMIAG IHLNHEDKSF SWMPYHHDMG
LIGFHLVPTA KGIHQFNMSP MKFVKRPNLW LDYITKHRIT LTGSPNFGYR LLLSRAKEEQ
FKKWDLRSLR LIFNGAEPIS VSLMREFMSK LKQCHLRKEA MFPVYGMAEA CLAVSFPKWN
DEPFVNCINR KTLVSESRVE KCLETDRQAM LLVEEGSPVA GMKIRIVDEV GNVVPEGIVG
EVQIYGENVT DGYIYNDRVT KESIQDGWLK TGDTGFLLND RLTIVGRIKD IIFVNGQNFY
AHDIEGIIEK IEGVKPGRIA ICGWHDEKEE KDKVALFSSL PIKQKDTNKI YSKIVSRVNE
LIGIPIDYVV LTPSIPKTTS GKVQRFVLID AFKNNKYEGK VLSRTDLSVG LQDENIVEKQ
EKKEKQKTME FGKYAEKIRE IWSEVLEIPL EHTPYNESFL SLGGTSIKAI QILGYLEDEL
SLTLSHDILI NCRTVNEMDE YIVKVVQGNE GIESGKVKVS SRKMSEEYEG DIAVIALSCR
FPDASTPEAF WENLVQGKCS IKEIPSDRWS VKDYYNQIPQ FGKTYCRKGA FIQNPYAFDA
SLFNISEEEA AIMDPQQRMI LELTYELIER AGYTTQMVDG EKVGLFIGAG MNSYYEYHLS
TLNRQNLQTF DSFKALPKVQ QEAIMNEWKK KFGIMAAHPN ILVDNILNMI AARTSQEFNF
KGPSMVVDTA CSSSLVTLHM AYESLKRGEC EVAIAGGINL LLTPAPYIFF SHAGALSSSG
RSMVFDEQAD GFVPGEGAGL VMLKTLKKAK ADGDKVLATI KASTINNDGR SIGVMAPNPD
GQREVIESLY VENEISPQTI QYVEAHGTGT RVGDPSEVRA IDTAFRKWNV GQQSIAIGSV
KANIGHLLGA AGIGSFIKVI MSLLYRKMPP NINIFTPNPM IKLEQTPFYL LDEPKEWSKE
KGVPRRAAIN SFGFGGTNCH LIVEEASETK EVKVNQAIEL PKHVLCLSAH SESALQQKVT
NLIACLEDSY EYSLGDVCYT ENVTRTPLKH RYSVIASSID DLIEKLKNSS LDNTMVQASP
KVALMFTGQG SQYAGMARQL YKLVPRFKKY VDDCSEAFYP ILNQSIVDLL YVEANESLLA
QTHITQPVVF TIDYALGKLL LDLGVKPACM LGHSVGEWVA ACLSEMITLQ DAARLVAIRG
ELMNEIQTTG AMTAIFSSIA TIEPLLEPFK DSLWFAGYNI THQVISGQAE AMEKFLSLLE
KKGIVFKKLK VSQAFHTPLM ETMLAPFKKE LSAITFNKPK IPIVSNVTAE YISQPIHPEY
WLEHILGTVK FEQSMKYVLD KGINIFVEAG PDKVLTSMAN GLISYKDKCL ISMIERKKEN
WDVYVNAVSK LYTLGVNIDW KTFMKDYGNE RVILPSYPFE HKIFKPDFGE ELRFMDWLYE
WKWEMETPLY IENDKRGSIL VFDDEQQVGK EVCGILEQIN TSVYVVIPGN EFHYDGDTRF
TIRPESIEDY MMMLQHIQGD SISIIHLWNG TKESVTPTLF LQDESALFQT SYSIILMAKA
LKIKAYKNVE FIVVTDRAFA VLQDEEIQKP QQSISAVIGQ VIDQENSEFS SCILDVDNRA
YENKFAELAE LIVRELSMGR QEEAIVAIRG GKRFVRKLER IVPSKKQEIT IQDNETYVIT
GGTGHIGGEI ARAFFKKAKV NLVLTGREQL PSREEWKKGN LSSKVVSKIN VIQDLENQGA
KVEYFAVDVT NEQKMKEMIQ AVKQLYGSIH GVVHAAGTIH HSAGKLLSKD LNDIQQVMSS
KVQGTIIADM VTRQEPLKFF VTLSSISASK KIWAANLGDY AAANAFLNGY SQYRKNTGAP
GRSLSINYSL WADKGLSEMF GDLSALAVKA QKLNPLSSEG GTQAFFEVLQ YEGKSVVHIL
DRQEDCVANN VAEKVVVLSP SNEPAHPVRY KTAKEIKEIV YQVIAAEINC DAESLEVTKN
FLELSIDSLK ATKVIAEIGK QLNTELYPTL LFEYQTPEAL AEYIEKTYAV NRQEKVEVRQ
KEKIGKDEES VKDIAIIGMS VRIPGANNLE EYWKILREGE CMIQEVPEDR WRKEYHYSSD
QGILHKTYSK HGGFIERPYD FDPLFFGMSP KEAEATDPQQ RIFMQVAWEA LQQAGYGGKH
RTNQIGVFVG CEQNTYMEHF IGYRYYMMMK DVLEKENTFA HLPLSDRQNL LKQMVRILEP
GQLVADAIAG NGLNEVAARV SHCLNLTGPS MIVNTACSSS LVALHHACQS IRQGETNMAI
AGGVNLNLSP TPFVSLSRVT ALSSNGNCYP FDARANGMVL SEGAGAVLLK SLEQAMEDGD
HIFAVIKGSA INNDGRSQGI TAPRPQGQAE VIRSAYLNAN VHPETVSYIE THGTATPLGD
PIEVEGMTRA FRTFTDKEGF CGIGSVKSSI GHMLSAAGIV SLVKVALAMK HKVIPHTVNY
DTPNPNLDFQ HSPFYVVAKQ PQIWGDEKQN HPLRAGVNAF GFGGTNAHVI LEEAPILQRK
VNHSVPEMKR PSLLLLTGRT EKVIQNVAMR LKEHIKQNPD LCVPEVCYSM NSSQKELSCK
VATVIKSKEH LYSVLEAIEM GRERSEVIKG RSNPNKRMTT HFLFDGTLCL DKAHIKQLND
TFSIFHAAYK KCLDVFETEE NENSTSYRQI QSFSCQYAIG KLLLHSGIQP KSFVCEGIGI
LVGAACTGII TVEAAIQFIK GNKNLTSEIL QNRNDTEYKY TIITPMGIIH KEHTDILKSL
CMTDMQYTIT TKDIAEYVDK NDVVIQCGIS KSDLQGRLIS MDIGKVSDSV HELLLCMAKL
YTLGVNYNSV AFFEGNENRI PLPTYPFENK MYKVTFEDEN IYKEALSNTE LHQKSNLVER
DVHKLVPLHL DETVQDSEKS KNMKQLNHDL VMLFGNIIQ
//
