ID A7WK53_LYSSH Unreviewed; 464 AA.
AC A7WK53;
DT 23-OCT-2007, integrated into UniProtKB/TrEMBL.
DT 23-OCT-2007, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Cry49Aa protein {ECO:0000313|EMBL:CAJ86541.1};
DE SubName: Full=Crystal toxin {ECO:0000313|EMBL:CAH56541.1};
GN Name=Cry49Aa1 {ECO:0000313|EMBL:CAH56541.1};
GN Synonyms=cry49Aa {ECO:0000313|EMBL:CAJ86541.1};
OS Lysinibacillus sphaericus (Bacillus sphaericus).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Lysinibacillus.
OX NCBI_TaxID=1421 {ECO:0000313|EMBL:CAH56541.1};
RN [1] {ECO:0000313|EMBL:CAJ86541.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=2173 {ECO:0000313|EMBL:CAJ86544.1}, 47-6B
RC {ECO:0000313|EMBL:CAJ86541.1}, and NHA15b
RC {ECO:0000313|EMBL:CAJ86543.1};
RA Jones G.W., Berry C.;
RT "Two new mosquitocidal toxins from Bacillus sphaericus.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CAH56541.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IAB59 {ECO:0000313|EMBL:CAH56541.1};
RA Jones G.W., Nielsen-Leroux C., Yang Y., Yuan Z., Dumas V.F., Monnerat R.G.,
RA Berry C.;
RT "A new Cry toxin with a unique two-component dependency from Bacillus
RT sphaericus.";
RL FASEB J. 21:4112-4120(2007).
RN [3] {ECO:0007829|PDB:7QA1}
RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 49-464, AND DISULFIDE BONDS.
RA Williamson L.J., Rizkallah P.J., Berry C., Oberthur D., Galchenkova M.,
RA Yefanov O., Bean R., Best H.L.;
RT "The structure of natural crystals of the Lysinibacillus sphaericus
RT Tpp49Aa1 pesticidal protein elucidated using serial femtosecond
RT crystallography at an X-ray free electron laser.";
RL Submitted (NOV-2021) to the PDB data bank.
RN [4] {ECO:0007829|PDB:8BEX, ECO:0007829|PDB:8BEY}
RP X-RAY CRYSTALLOGRAPHY (1.62 ANGSTROMS) OF 49-464, AND DISULFIDE BONDS.
RA Williamson L.J., Rizkallah P.J., Berry C., Oberthur D., Galchenkova M.,
RA Yefanov O., Bean R.;
RT "Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein
RT elucidated from natural crystals using MHz-SFX.";
RL Submitted (OCT-2022) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Spore, perispore {ECO:0000256|ARBA:ARBA00024181}.
CC -!- SIMILARITY: Belongs to the toxin_10 family.
CC {ECO:0000256|ARBA:ARBA00006163}.
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DR EMBL; AJ841948; CAH56541.1; -; Genomic_DNA.
DR EMBL; AM237201; CAJ86541.1; -; Genomic_DNA.
DR EMBL; AM237203; CAJ86543.1; -; Genomic_DNA.
DR EMBL; AM237204; CAJ86544.1; -; Genomic_DNA.
DR PDB; 7QA1; X-ray; 2.20 A; A/B=49-464.
DR PDB; 8BEX; X-ray; 1.78 A; A/B=49-464.
DR PDB; 8BEY; X-ray; 1.62 A; A/B=49-464.
DR PDB; 8BEZ; X-ray; 1.75 A; A/B=49-464.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR008872; Toxin_P42.
DR Pfam; PF05431; Toxin_10; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:7QA1, ECO:0007829|PDB:8BEX};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Toxin {ECO:0000256|ARBA:ARBA00022656};
KW Virulence {ECO:0000256|ARBA:ARBA00023026}.
FT DOMAIN 250..416
FT /note="Insecticidal crystal toxin"
FT /evidence="ECO:0000259|Pfam:PF05431"
FT DISULFID 91..183
FT /evidence="ECO:0007829|PDB:7QA1, ECO:0007829|PDB:8BEX"
SQ SEQUENCE 464 AA; 53276 MW; C2B79598C330C70B CRC64;
MENQIKEEFN KNNHGIPSDC SCIKEGDDYN SLTEINAKEF SYCSPNMFNL NLPEQSTRFQ
TIASIHSNNC SFEILNNDPG YIYGDSVDGE CRIAVAHREL GNGLERTGDD RFLFIFYALD
NNNFIIANRH DGFVLQFLIA NGQGVIVSRE YQPNIHQEFT IQSINSDTFR LHSRDTNTFA
TVCWAQFNSW TKIVSRVDNP GAPNANLKHR SLLTDINMPQ LPSLTPLQPL PRLTELEDGG
LSPAQAPRAI IGRTLIPCLF VNDPVLRLEN RIKQSPYYVL EHRQYWHRIW TDIFTAGERR
EYREVTGINN NAQNDMNKMI NITIGADGPN RLRFGNLSTP FRQQIIDNSN TLGSFANTNY
GTRTDIVNVF NSEFHQVRYA RFVKAYEYRL TRADGSQVGT PWVVLDRKEM DLRTYPHNMA
ITLENVKIDN ADNSYDLSIW KTPLKLKDGK IIIENHENSK PYYN
//
