ID A7XUU4_COCPS Unreviewed; 603 AA.
AC A7XUU4; E9CXC6;
DT 23-OCT-2007, integrated into UniProtKB/TrEMBL.
DT 23-OCT-2007, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093};
DE EC=3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093};
GN ORFNames=CPSG_02109 {ECO:0000313|EMBL:EFW21952.1};
OS Coccidioides posadasii (strain RMSCC 757 / Silveira) (Valley fever fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX NCBI_TaxID=443226 {ECO:0000313|EMBL:ABU87865.1};
RN [1] {ECO:0000313|Proteomes:UP000002497}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RMSCC 757 / Silveira {ECO:0000313|Proteomes:UP000002497};
RX PubMed=20516208; DOI=10.1101/gr.103911.109;
RA Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA Taylor J.W., Rounsley S.D.;
RT "Population genomic sequencing of Coccidioides fungi reveals recent
RT hybridization and transposon control.";
RL Genome Res. 20:938-946(2010).
RN [2] {ECO:0000313|EMBL:ABU87865.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Silveira {ECO:0000313|EMBL:ABU87865.1};
RX PubMed=20109094; DOI=10.3109/13693780903496609;
RA Lunetta J.M., Johnson S.M., Pappagianis D.;
RT "Molecular cloning, characterization and expression analysis of two beta-N-
RT acetylhexosaminidase homologs of Coccidioides posadasii.";
RL Med. Mycol. 48:744-756(2010).
RN [3] {ECO:0000313|Proteomes:UP000002497}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RMSCC 757 / Silveira {ECO:0000313|Proteomes:UP000002497};
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Neafsey D., Orbach M., Henn M.R., Cole G.T., Galgiani J., Gardner M.J.,
RA Kirkland T.N., Taylor J.W., Young S.K., Zeng Q., Koehrsen M., Alvarado L.,
RA Berlin A., Borenstein D., Chapman S.B., Chen Z., Engels R., Freedman E.,
RA Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D.,
RA Howarth C., Jen D., Larson L., Mehta T., Neiman D., Park D., Pearson M.,
RA Richards J., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C.,
RA Sykes S., Walk T., White J., Yandava C., Haas B., Nusbaum C., Birren B.;
RT "The genome sequence of Coccidioides posadasii strain Silveira.";
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:EFW21952.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Silveira {ECO:0000313|EMBL:EFW21952.1};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease.;
RA Neafsey D., Orbach M., Henn M.R., Cole G.T., Galgiani J., Gardner M.J.,
RA Kirkland T.N., Taylor J.W., Young S.K., Zeng Q., Koehrsen M., Alvarado L.,
RA Berlin A., Borenstein D., Chapman S.B., Chen Z., Engels R., Freedman E.,
RA Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D.,
RA Howarth C., Jen D., Larson L., Mehta T., Neiman D., Park D., Pearson M.,
RA Richards J., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C.,
RA Sykes S., Walk T., White J., Yandava C., Haas B., Nusbaum C., Birren B.;
RT "The Genome Sequence of Coccidioides posadasii strain Silveira.";
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:ABU87865.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Silveira {ECO:0000313|EMBL:ABU87865.1};
RA Lunetta J.M., Johnson S.M., Pappagianis D.;
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine
CC residues in N-acetyl-beta-D-hexosaminides.; EC=3.2.1.52;
CC Evidence={ECO:0000256|ARBA:ARBA00001231,
CC ECO:0000256|PIRNR:PIRNR001093};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 20 family.
CC {ECO:0000256|ARBA:ARBA00006285, ECO:0000256|PIRNR:PIRNR001093}.
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DR EMBL; EU099030; ABU87865.1; -; mRNA.
DR EMBL; GL636487; EFW21952.1; -; Genomic_DNA.
DR STRING; 443226.A7XUU4; -.
DR CAZy; GH20; Glycoside Hydrolase Family 20.
DR VEuPathDB; FungiDB:CPSG_02109; -.
DR VEuPathDB; FungiDB:D8B26_002055; -.
DR eggNOG; KOG2499; Eukaryota.
DR OMA; GHDVVMC; -.
DR BRENDA; 3.2.1.52; 9184.
DR Proteomes; UP000002497; Unassembled WGS sequence.
DR GO; GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102148; F:N-acetyl-beta-D-galactosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd06562; GH20_HexA_HexB-like; 1.
DR Gene3D; 3.30.379.10; Chitobiase/beta-hexosaminidase domain 2-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR025705; Beta_hexosaminidase_sua/sub.
DR InterPro; IPR015883; Glyco_hydro_20_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR029018; Hex-like_dom2.
DR InterPro; IPR029019; HEX_eukaryotic_N.
DR PANTHER; PTHR22600; BETA-HEXOSAMINIDASE; 1.
DR PANTHER; PTHR22600:SF56; BETA-HEXOSAMINIDASE; 1.
DR Pfam; PF00728; Glyco_hydro_20; 1.
DR Pfam; PF14845; Glycohydro_20b2; 1.
DR PIRSF; PIRSF001093; B-hxosamndse_ab_euk; 1.
DR PRINTS; PR00738; GLHYDRLASE20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF55545; beta-N-acetylhexosaminidase-like domain; 1.
PE 2: Evidence at transcript level;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR001093};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR001093};
KW Reference proteome {ECO:0000313|Proteomes:UP000002497};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..603
FT /note="Beta-hexosaminidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010958698"
FT DOMAIN 20..189
FT /note="Beta-hexosaminidase eukaryotic type N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14845"
FT DOMAIN 213..557
FT /note="Glycoside hydrolase family 20 catalytic"
FT /evidence="ECO:0000259|Pfam:PF00728"
FT ACT_SITE 374
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-1"
SQ SEQUENCE 603 AA; 68499 MW; 39B04D07626A491C CRC64;
MRFLVVSYFL LTAGSFVSAI WPEPAHYTHG DTVVLLDPSV RFTYQRGEFR VYDEGGSYFT
ASMDYGRLMR MLEPQWFGIG NLHPNSEKSV DDILKKAIKR TRKELRRTRF VPWKFHPRNQ
SFELRSLRRE EAIKEILIEE APSDDEILSR DYISGDESYE IRITKKGKAT ILTASPIGTL
RALQTLPQLF YAHSFGGVYT PYAPISISDK PKWSHRGLNL DISRNPIGPN DVKRTIDAMA
SVKLNRLHIH ATDSQSWPLD IPSLPSLAAK AAYHPRLVWS SSDLRNVQKY GLARGVSTFI
EIDMPGHTGS IGHAFPDLLV AFGNDSWDKY ALEPPCGQVK LNDSAVRRFF DTVMADILPR
VSPFTAYFHT GGDEFNLQSY MLEETIRSND PKVLKPLLQD FVNRVHMQIM EAGLTPIVWE
ELVLDWDLTF PSQQSETRGI IVQAWRNSTA VRQILEKGYR TIFGSGDAWY LDCGVGGFIN
PRPGSGAVKE PYLDWCSPTK NWRHIYTYNP LEGIPEELQS LLEGGETHMW AENVDPVNMD
QMIWPRAASA AEVLWSGPRA RDDIKEASHR LGKWRERAVI DMGVGASMVQ MTYCLMREGS
CEL
//
