ID RSMB_ECOHS Reviewed; 429 AA.
AC A8A593;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 1.
DT 01-MAY-2013, entry version 45.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase B;
DE EC=2.1.1.176;
DE AltName: Full=16S rRNA m5C967 methyltransferase;
DE AltName: Full=rRNA (cytosine-C(5)-)-methyltransferase RsmB;
GN Name=rsmB; Synonyms=sun; OrderedLocusNames=EcHS_A3482;
OS Escherichia coli O9:H4 (strain HS).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=331112;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HS;
RX PubMed=18676672; DOI=10.1128/JB.00619-08;
RA Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA Henderson I.R., Sperandio V., Ravel J.;
RT "The pangenome structure of Escherichia coli: comparative genomic
RT analysis of E. coli commensal and pathogenic isolates.";
RL J. Bacteriol. 190:6881-6893(2008).
CC -!- FUNCTION: Specifically methylates the cytosine at position 967
CC (m5C967) of 16S rRNA (By similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + cytosine(967) in 16S
CC rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S
CC rRNA.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmB/NOP
CC family.
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DR EMBL; CP000802; ABV07697.1; -; Genomic_DNA.
DR RefSeq; YP_001460080.1; NC_009800.1.
DR ProteinModelPortal; A8A593; -.
DR SMR; A8A593; 5-428.
DR STRING; 331112.EcHS_A3482; -.
DR EnsemblBacteria; ABV07697; ABV07697; EcHS_A3482.
DR GeneID; 5591816; -.
DR KEGG; ecx:EcHS_A3482; -.
DR PATRIC; 18316924; VBIEscCol77814_3390.
DR eggNOG; COG0144; -.
DR HOGENOM; HOG000037300; -.
DR KO; K03500; -.
DR OMA; KPPMWLR; -.
DR ProtClustDB; PRK10901; -.
DR BioCyc; ECOL331112:GHHI-3521-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016434; F:rRNA (cytosine) methyltransferase activity; IEA:HAMAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
DR Gene3D; 1.10.940.10; -; 1.
DR HAMAP; MF_01856; 16SrRNA_methyltr_B; 1; -.
DR InterPro; IPR001678; Fmu/NOL1/Nop2p.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR004573; rRNA_ssu_MeTfrase_B.
DR InterPro; IPR023541; rRNA_ssu_MeTfrase_B_ent.
DR Pfam; PF01189; Nol1_Nop2_Fmu; 1.
DR Pfam; PF01029; NusB; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF48013; NusB_RsmB_TIM44; 1.
DR TIGRFAMs; TIGR00563; rsmB; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Methyltransferase; RNA-binding;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1 429 Ribosomal RNA small subunit
FT methyltransferase B.
FT /FTId=PRO_0000366159.
FT REGION 254 260 S-adenosyl-L-methionine binding (By
FT similarity).
FT ACT_SITE 375 375 Nucleophile (Potential).
FT BINDING 277 277 S-adenosyl-L-methionine (By similarity).
FT BINDING 303 303 S-adenosyl-L-methionine (By similarity).
FT BINDING 322 322 S-adenosyl-L-methionine (By similarity).
SQ SEQUENCE 429 AA; 48348 MW; 1F516223C84D80F8 CRC64;
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG VLRTLSQLDW
LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA ETVEGAIAIK RPQLKGLING
VLRQFQRQQE ELLAEFNASD ARYLHPSWLL KRLQKAYPEQ WQSIVEANNQ RPPMWLRINR
THHSRDSWLA LLDEAGMKGF PHADYPDAVR LETPAPVHAL PGFEDGWVTV QDASAQGCMT
WLAPQNGEHI LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA QLQSEILDAI
WPHLKTGGTL VYATCSVLPE ENSLQIKAFL QRTADAELCE TGTPEQPGKQ NLPGAEEGDG
FFYAKLIKK
//
