ID A8B3I1_9FLAV Unreviewed; 1167 AA.
AC A8B3I1;
DT 13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2007, sequence version 1.
DT 27-MAR-2024, entry version 83.
DE RecName: Full=Genome polyprotein {ECO:0000256|ARBA:ARBA00020107};
DE Flags: Fragment;
GN Name=E {ECO:0000313|EMBL:ABM65593.1};
OS Murray Valley encephalitis virus.
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Flasuviricetes;
OC Amarillovirales; Flaviviridae; Orthoflavivirus; Orthoflavivirus murrayense.
OX NCBI_TaxID=11079 {ECO:0000313|EMBL:ABM65593.1};
RN [1] {ECO:0000313|EMBL:ABM65593.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=MK6684 {ECO:0000313|EMBL:ABM65593.1};
RA Mackenzie J.S., Hall R.A., Poidinger M., Khromykh T.I., Johansen C.A.;
RT "Murray Valley encephalitis virus in Papua New Guinea.";
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of
CC the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.;
CC EC=3.4.21.91; Evidence={ECO:0000256|ARBA:ARBA00024468};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-
CC diphosphate + H(+) + phosphate; Xref=Rhea:RHEA:23680,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57930, ChEBI:CHEBI:61557; EC=3.6.1.15;
CC Evidence={ECO:0000256|ARBA:ARBA00001491};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004477}. Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004367}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004367}; Lumenal side
CC {ECO:0000256|ARBA:ARBA00004367}. Host endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004153}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004153}. Host endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00023443}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00023443}; Lumenal side
CC {ECO:0000256|ARBA:ARBA00023443}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}. Secreted
CC {ECO:0000256|ARBA:ARBA00004613}. Virion membrane
CC {ECO:0000256|ARBA:ARBA00004385}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004385}.
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DR EMBL; EF015075; ABM65593.1; -; Genomic_RNA.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0017111; F:ribonucleoside triphosphate phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0039654; P:fusion of virus membrane with host endosome membrane; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR CDD; cd12149; Flavi_E_C; 1.
DR CDD; cd17038; Flavi_M; 1.
DR Gene3D; 1.20.1280.260; -; 1.
DR Gene3D; 2.60.40.350; -; 1.
DR Gene3D; 1.10.8.970; Flavivirus envelope glycoprotein M-like; 1.
DR Gene3D; 2.60.260.50; Flavivirus polyprotein propeptide domain; 1.
DR Gene3D; 2.60.98.10; Tick-borne Encephalitis virus Glycoprotein, domain 1; 1.
DR Gene3D; 3.30.67.10; Viral Envelope Glycoprotein, domain 2; 1.
DR Gene3D; 3.30.387.10; Viral Envelope Glycoprotein, domain 3; 1.
DR InterPro; IPR000069; Env_glycoprot_M_flavivir.
DR InterPro; IPR038302; Env_glycoprot_M_sf_flavivir.
DR InterPro; IPR013755; Flav_gly_cen_dom_subdom1.
DR InterPro; IPR027287; Flavi_E_Ig-like.
DR InterPro; IPR026470; Flavi_E_Stem/Anchor_dom.
DR InterPro; IPR038345; Flavi_E_Stem/Anchor_dom_sf.
DR InterPro; IPR011998; Flavi_Glycoprot_E_cen/dimer.
DR InterPro; IPR001157; Flavi_NS1.
DR InterPro; IPR000752; Flavi_NS2A.
DR InterPro; IPR002535; Flavi_propep.
DR InterPro; IPR038688; Flavi_propep_sf.
DR InterPro; IPR000336; Flavivir/Alphavir_Ig-like_sf.
DR InterPro; IPR036253; Glycoprot_cen/dimer_sf.
DR InterPro; IPR038055; Glycoprot_E_dimer_dom.
DR InterPro; IPR013756; GlyE_cen_dom_subdom2.
DR InterPro; IPR014756; Ig_E-set.
DR NCBIfam; TIGR04240; flavi_E_stem; 1.
DR Pfam; PF21659; Flavi_E_stem; 1.
DR Pfam; PF02832; Flavi_glycop_C; 1.
DR Pfam; PF00869; Flavi_glycoprot; 1.
DR Pfam; PF01004; Flavi_M; 1.
DR Pfam; PF00948; Flavi_NS1; 1.
DR Pfam; PF01005; Flavi_NS2A; 1.
DR Pfam; PF01570; Flavi_propep; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF56983; Viral glycoprotein, central and dimerisation domains; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Capsid protein {ECO:0000256|ARBA:ARBA00022561};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Fusion of virus membrane with host endosomal membrane
KW {ECO:0000256|ARBA:ARBA00022510};
KW Fusion of virus membrane with host membrane
KW {ECO:0000256|ARBA:ARBA00022506};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Host endoplasmic reticulum {ECO:0000256|ARBA:ARBA00023184};
KW Host membrane {ECO:0000256|ARBA:ARBA00022870};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Viral attachment to host cell {ECO:0000256|ARBA:ARBA00022804};
KW Viral envelope protein {ECO:0000313|EMBL:ABM65593.1};
KW Viral penetration into host cytoplasm {ECO:0000256|ARBA:ARBA00022595};
KW Virion {ECO:0000256|ARBA:ARBA00022844};
KW Virus entry into host cell {ECO:0000256|ARBA:ARBA00023296}.
FT TRANSMEM 628..649
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 655..676
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 18..99
FT /note="Flavivirus polyprotein propeptide"
FT /evidence="ECO:0000259|Pfam:PF01570"
FT DOMAIN 102..175
FT /note="Envelope glycoprotein M flavivirus"
FT /evidence="ECO:0000259|Pfam:PF01004"
FT DOMAIN 178..474
FT /note="Envelope glycoprotein E central and dimerisation"
FT /evidence="ECO:0000259|Pfam:PF00869"
FT DOMAIN 477..574
FT /note="Glycoprotein E immunoglobulin-like"
FT /evidence="ECO:0000259|Pfam:PF02832"
FT DOMAIN 577..671
FT /note="Flavivirus envelope glycoprotein E Stem/Anchor"
FT /evidence="ECO:0000259|Pfam:PF21659"
FT DOMAIN 679..1028
FT /note="Non-structural protein NS1 flavivirus"
FT /evidence="ECO:0000259|Pfam:PF00948"
FT DOMAIN 1040..1143
FT /note="Flavivirus non-structural protein NS2A"
FT /evidence="ECO:0000259|Pfam:PF01005"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ABM65593.1"
FT NON_TER 1167
FT /evidence="ECO:0000313|EMBL:ABM65593.1"
SQ SEQUENCE 1167 AA; 127788 MW; 51F7CC7FFB6E8BDE CRC64;
MLIGLSAALK LSTFQGKIMM TVNATDIADV IAIPTPKGPN QCWIRAIDIG FMCDDTITYE
CPKLESGNDP EDIDCWCDKQ AVYVNYGRCT RARHSKRSRR SITVQTHGES TLVNKKDAWL
DSTKATRYLT KTENWIIRNP GYALVAVVLG WMLGSNTGQK VIFTVLLLLV APAYSFNCLG
MSSRDFIEGA SGATWVDLVL EGDSCITIMA ADKPTLDIRM MNIEATNLAL VRNYCYAAIV
SDVSTVSNCP TTGESHNTKR ADHNYLCKRG VTDRGWGNGC GLFGKGSIDT CAKFTCSSSA
AGRLILPENI KYEVGIFVHG STDSTSHGNY STQIGANQAA RFTISPNAPA ITAKMGDYGE
VTVECEPRSG LNTEAYYVMT IGTKHFLVHR EWFNDLLLPW TSPSSTEWRN REILMEFEEP
HATKQSVVAL GSQEGALHQA LAGAIPVEFA SSTLKLTSGH LKCRVKMEKL KLKGTTYGMC
TEKFTFSKNP ADTGHGTVVL ELQYTGSDGP CKIPISSVAS LNDMTPVGRM VTANPYVASS
TANAKVLVEI EPPFGDSYIV VGRGDKQINH HWHKEGSSIG KAFSTTLKGA QRLAALGDTA
WDFGSVGGVF NSIGKAVHQV FGGAFRTLFG GMSWISQGLL GALLLWMGVN ARDKSIALAF
LATGGVLLFL ATNVHADTGC AIDITRRELK CGSGIFIHND VEAWIDRYKY LPETPKQLAK
VVENAHKSGI CGIRSVNRFE HQMWESVRDE LNALLKENAI DLSVVVEKQK GMYRAAPNRL
KLTVEELDIG WKAWGKSLLF AAELANSTFV VDGPETAECP NSKRAWNSFE IEDFGFGITS
TRVWLKLREE NTSECDSTII GTAVKGNHAV HSDLSYWIES GLNGTWKLER AIFGEVKSCT
WPETHTLWGD AVEETELIIP VTLAGPRSKH NRREGYKVQV QGPWDEEDIK LDFDYCPGTT
VTVSEHCGKR GPSVRTTTDS GKLVTDWCCR SCTLPPLRFT TASGCWYGME IRPMKHDEST
LVKSRVQALN GDMIDPFQLG LLVMFLATQE VLRKRWTARL TLPAAVGALL VLLLGGITYT
DLVRYLILVG SAFAESNNGG DVIHLALIAV FKVQPAFLVA SLTRSRWTNQ ENLVLVLGAA
FFQMAASDLE LSIPGLLNSA ATAWMVL
//
