ID A8BTN5_GIAIC Unreviewed; 674 AA.
AC A8BTN5;
DT 13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2007, sequence version 1.
DT 24-JAN-2024, entry version 60.
DE RecName: Full=polynucleotide adenylyltransferase {ECO:0000256|ARBA:ARBA00012388};
DE EC=2.7.7.19 {ECO:0000256|ARBA:ARBA00012388};
GN ORFNames=GL50803_13749 {ECO:0000313|EMBL:EDO77239.1};
OS Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=184922 {ECO:0000313|EMBL:EDO77239.1};
RN [1] {ECO:0000313|EMBL:EDO77239.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WB C6 {ECO:0000313|EMBL:EDO77239.1};
RX PubMed=17901334; DOI=10.1126/science.1143837;
RA Morrison H.G., McArthur A.G., Gillin F.D., Aley S.B., Adam R.D.,
RA Olsen G.J., Best A.A., Cande W.Z., Chen F., Cipriano M.J., Davids B.J.,
RA Dawson S.C., Elmendorf H.G., Hehl A.B., Holder M.E., Huse S.M., Kim U.U.,
RA Lasek-Nesselquist E., Manning G., Nigam A., Nixon J.E., Palm D.,
RA Passamaneck N.E., Prabhu A., Reich C.I., Reiner D.S., Samuelson J.,
RA Svard S.G., Sogin M.L.;
RT "Genomic minimalism in the early diverging intestinal parasite Giardia
RT lamblia.";
RL Science 317:1921-1926(2007).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the poly(A) polymerase family.
CC {ECO:0000256|ARBA:ARBA00010912}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDO77239.1}.
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DR EMBL; AACB02000044; EDO77239.1; -; Genomic_DNA.
DR RefSeq; XP_001704913.1; XM_001704861.1.
DR AlphaFoldDB; A8BTN5; -.
DR STRING; 184922.A8BTN5; -.
DR EnsemblProtists; EDO77239; EDO77239; GL50803_13749.
DR GeneID; 5697771; -.
DR KEGG; gla:GL50803_0013749; -.
DR VEuPathDB; GiardiaDB:GL50803_13749; -.
DR HOGENOM; CLU_407968_0_0_1; -.
DR OMA; CTANMHE; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:1990817; F:poly(A) RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR CDD; cd05402; NT_PAP_TUTase; 1.
DR Gene3D; 1.10.1410.10; -; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR007012; PolA_pol_cen_dom.
DR InterPro; IPR048840; PolA_pol_NTPase.
DR PANTHER; PTHR10682; POLY A POLYMERASE; 1.
DR PANTHER; PTHR10682:SF10; POLYNUCLEOTIDE ADENYLYLTRANSFERASE; 1.
DR Pfam; PF04928; PAP_central; 1.
DR Pfam; PF20750; PAP_NTPase; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81631; PAP/OAS1 substrate-binding domain; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 94..270
FT /note="Poly(A) polymerase nucleotidyltransferase"
FT /evidence="ECO:0000259|Pfam:PF20750"
FT DOMAIN 286..323
FT /note="Poly(A) polymerase central"
FT /evidence="ECO:0000259|Pfam:PF04928"
SQ SEQUENCE 674 AA; 77270 MW; 9415081CD3524B0C CRC64;
MLYSGASSSA CSVTSTVKPA LAVQHDFLGQ VSVERRKRCI YTMQRQKLNN CSSFEQEMTS
HLGGQSPLID AEVPLQPLPE GHKLNKEQFQ LVDNLLLSYL KNCKLYVSSE ETRKKEAILR
NLELIISDWV YETYKDAFLA NMQFYRKQPN MPRSERLVRL YPFGSYYLGI NEPSSDVDTV
VIFPQYVRIA DFFDKFPTII GQMPQVSYFD CIRDAKVPLI TLTYDSVDFD LSAAVMAVNR
VTDAIPFLDP ECTANMHEKS ILSLNGYRTN IHVKSQFAGN NIFYSTFQNA VRALRLWCKR
KNIYSNRCGF FGGINCIILV ANVMLRSSFE LLQLDVEQQN LEYQASLSTA QGDLTSPSDP
QSPYIQYNSD SILELREAVP LTLWLYNIYY YYSRLDWSTK AIQLPNPVQA SAATRLSMTA
QSWVRRDKDK MCLLTAVEPY FNSTDKIFET TFDTILTNFR QGVKAILDIV RGVVAAYSFG
LEIARPLLRP SLWDTFFYNS FLEFFSNPGY RPNIMYLLIT RNTMSSNSMV ELDKVKSFLE
SRINRICRRL LTSLQPAVGN NARLIRVHPL PTWYDSTSLG ELEHGKHYFY IGMETSTTGD
LRGLSERLSQ EVRDFARECK GYLSEKRMLL GMESIDKMLQ IRITTYKDLP EALRGLSPPE
QAKTLYCTSG QQIE
//
