ID A8C544_PLAMA Unreviewed; 226 AA.
AC A8C544;
DT 13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2007, sequence version 1.
DT 24-JAN-2024, entry version 40.
DE RecName: Full=Bifunctional dihydrofolate reductase-thymidylate synthase {ECO:0000256|ARBA:ARBA00019798};
DE EC=1.5.1.3 {ECO:0000256|ARBA:ARBA00012856};
DE EC=2.1.1.45 {ECO:0000256|ARBA:ARBA00011947};
DE Flags: Fragment;
OS Plasmodium malariae.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Plasmodium).
OX NCBI_TaxID=5858 {ECO:0000313|EMBL:ABQ23921.1};
RN [1] {ECO:0000313|EMBL:ABQ23921.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Af16 {ECO:0000313|EMBL:ABQ23926.1}, Au237
RC {ECO:0000313|EMBL:ABQ23933.1}, Av427 {ECO:0000313|EMBL:ABQ23921.1},
RC and Vp64 {ECO:0000313|EMBL:ABQ23932.1};
RX PubMed=17682097; DOI=10.1128/AAC.00234-07;
RA Tanomsing N., Imwong M., Pukrittayakamee S., Chotivanich K.,
RA Looareesuwan S., Mayxay M., Dolecek C., Hien T.T., do Rosario V.E.,
RA Arez A.P., Michon P., Snounou G., White N.J., Day N.P.;
RT "Genetic analysis of the dihydrofolate reductase-thymidylate synthase gene
RT from geographically diverse isolates of Plasmodium malariae.";
RL Antimicrob. Agents Chemother. 51:3523-3530(2007).
CC -!- FUNCTION: Bifunctional enzyme. Involved in de novo dTMP biosynthesis.
CC Key enzyme in folate metabolism. Catalyzes an essential reaction for de
CC novo glycine and purine synthesis, DNA precursor synthesis, and for the
CC conversion of dUMP to dTMP. {ECO:0000256|ARBA:ARBA00025154}.
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-
CC tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC {ECO:0000256|ARBA:ARBA00004903}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC {ECO:0000256|RuleBase:RU004474}.
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DR EMBL; EF206328; ABQ23921.1; -; Genomic_DNA.
DR EMBL; EF206333; ABQ23926.1; -; Genomic_DNA.
DR EMBL; EF206339; ABQ23932.1; -; Genomic_DNA.
DR EMBL; EF206340; ABQ23933.1; -; Genomic_DNA.
DR AlphaFoldDB; A8C544; -.
DR UniPathway; UPA00077; UER00158.
DR GO; GO:0004146; F:dihydrofolate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00209; DHFR; 1.
DR Gene3D; 3.40.430.10; Dihydrofolate Reductase, subunit A; 1.
DR InterPro; IPR012259; DHFR.
DR InterPro; IPR024072; DHFR-like_dom_sf.
DR InterPro; IPR017925; DHFR_CS.
DR InterPro; IPR001796; DHFR_dom.
DR PANTHER; PTHR48069; DIHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR48069:SF3; DIHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF00186; DHFR_1; 1.
DR PRINTS; PR00070; DHFR.
DR SUPFAM; SSF53597; Dihydrofolate reductase-like; 1.
DR PROSITE; PS00075; DHFR_1; 1.
DR PROSITE; PS51330; DHFR_2; 1.
PE 3: Inferred from homology;
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 1..226
FT /note="DHFR"
FT /evidence="ECO:0000259|PROSITE:PS51330"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ABQ23921.1"
FT NON_TER 226
FT /evidence="ECO:0000313|EMBL:ABQ23921.1"
SQ SEQUENCE 226 AA; 26247 MW; E52FB9F9BDF66FFA CRC64;
DIYAICACCK VPNHGEGKKN EIFSTKTFRG LGNKGCLPWK SNSLDMKYFS SVTTYVNEMK
YKKLKYKREK YLEKEISNEN SSTVFENISL LSSSKLQNVV VMGRSSWVSI PKQYKPLPNR
INVVLSRTLK KEDVKEDIFI INNMDQLVLL LKKLNYYKCF IIGGAIVYKE CLERNLIKQI
YFTRINNVYE CDVFFPEIDE NVFQITSVSD VYTSNCTSLD FVIFSK
//
