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Database: UniProt
Entry: A8FD15
LinkDB: A8FD15
Original site: A8FD15 
ID   PYRC_BACP2              Reviewed;         428 AA.
AC   A8FD15;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   26-NOV-2014, entry version 49.
DE   RecName: Full=Dihydroorotase {ECO:0000255|HAMAP-Rule:MF_00220};
DE            Short=DHOase {ECO:0000255|HAMAP-Rule:MF_00220};
DE            EC=3.5.2.3 {ECO:0000255|HAMAP-Rule:MF_00220};
GN   Name=pyrC {ECO:0000255|HAMAP-Rule:MF_00220};
GN   OrderedLocusNames=BPUM_1449;
OS   Bacillus pumilus (strain SAFR-032).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=315750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032;
RX   PubMed=17895969; DOI=10.1371/journal.pone.0000928;
RA   Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D.,
RA   Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C.,
RA   Dinh H., Lee S., Nazareth L., Blyth P., Holder M., Buhay C.,
RA   Tirumalai M.R., Liu Y., Dasgupta I., Bokhetache L., Fujita M.,
RA   Karouia F., Eswara Moorthy P., Siefert J., Uzman A., Buzumbo P.,
RA   Verma A., Zwiya H., McWilliams B.D., Olowu A., Clinkenbeard K.D.,
RA   Newcombe D., Golebiewski L., Petrosino J.F., Nicholson W.L., Fox G.E.,
RA   Venkateswaran K., Highlander S.K., Weinstock G.M.;
RT   "Paradoxical DNA repair and peroxide resistance gene conservation in
RT   Bacillus pumilus SAFR-032.";
RL   PLoS ONE 2:E928-E928(2007).
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC       aspartate. {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00220};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_00220};
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
CC       {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- SIMILARITY: Belongs to the DHOase family. Type 2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00220}.
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DR   EMBL; CP000813; ABV62132.1; -; Genomic_DNA.
DR   RefSeq; WP_012009895.1; NC_009848.1.
DR   RefSeq; YP_001486692.1; NC_009848.1.
DR   ProteinModelPortal; A8FD15; -.
DR   STRING; 315750.BPUM_1449; -.
DR   MEROPS; M38.972; -.
DR   EnsemblBacteria; ABV62132; ABV62132; BPUM_1449.
DR   GeneID; 5620716; -.
DR   KEGG; bpu:BPUM_1449; -.
DR   PATRIC; 18966735; VBIBacPum16546_1464.
DR   eggNOG; COG0044; -.
DR   HOGENOM; HOG000219142; -.
DR   KO; K01465; -.
DR   OMA; PLLYTRF; -.
DR   OrthoDB; EOG6KHFW6; -.
DR   BioCyc; BPUM315750:GH6N-1504-MONOMER; -.
DR   UniPathway; UPA00070; UER00117.
DR   GO; GO:0004151; F:dihydroorotase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.40.10; -; 1.
DR   HAMAP; MF_00220_B; PyrC_type2_B; 1.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR004722; DHOase.
DR   InterPro; IPR002195; Dihydroorotase_CS.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   TIGRFAMs; TIGR00857; pyrC_multi; 1.
DR   PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR   PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW   Reference proteome; Zinc.
FT   CHAIN         1    428       Dihydroorotase.
FT                                /FTId=PRO_1000058653.
FT   METAL        59     59       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL        61     61       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       141    141       Zinc 1; via carbamate group.
FT                                {ECO:0000255|HAMAP-Rule:MF_00220}.
FT   METAL       141    141       Zinc 2; via carbamate group.
FT                                {ECO:0000255|HAMAP-Rule:MF_00220}.
FT   METAL       178    178       Zinc 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       231    231       Zinc 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       304    304       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   MOD_RES     141    141       N6-carboxylysine. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
SQ   SEQUENCE   428 AA;  46993 MW;  B50A9168280389E2 CRC64;
     MSYLIKNGFI LTSTGEKVQQ DIRVEGEVIQ AIGHLEREDG EEVIDAKGLF VSPGLIDLHV
     HLREPGGEKK ETIETGSKAA ARGGYTTIAA MPNTRPVPDT KEQMEWLMNR IEETSSVRVL
     PYASITTRQI GEEMTDFAAL KEAGAFAFTD DGVGIQTAGM MYEAMKKAAS LDQAIVAHCE
     DNSLIYGGCV HEGEFSKANG LNGIPSICES VHIARDVLLA EAANCHYHVC HISTKESVRV
     VRDAKKAGIR VTAEVSPHHL LLCDADIPGL DTNYKMNPPL RGKEDREALI EGLLDGTIDF
     IATDHAPHTE EEKNETMQRA PFGIVGLETA FPLLYTRFVK TGEWTLKELV DYMTIKPAEA
     FSLPYGKLEK GQIADITLID LNKEMAIDKK SFLSKGQNTP FDKLTVSGWP VMTLASGKVV
     YEEGRLVK
//
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