ID A8U1Y1_9PROT Unreviewed; 675 AA.
AC A8U1Y1;
DT 15-JAN-2008, integrated into UniProtKB/TrEMBL.
DT 15-JAN-2008, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:EDP61702.1};
GN ORFNames=BAL199_00325 {ECO:0000313|EMBL:EDP61702.1};
OS alpha proteobacterium BAL199.
OC Bacteria; Pseudomonadota; Alphaproteobacteria.
OX NCBI_TaxID=331869 {ECO:0000313|EMBL:EDP61702.1, ECO:0000313|Proteomes:UP000003417};
RN [1] {ECO:0000313|EMBL:EDP61702.1, ECO:0000313|Proteomes:UP000003417}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BAL199 {ECO:0000313|EMBL:EDP61702.1,
RC ECO:0000313|Proteomes:UP000003417};
RA Hagstrom A., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G.,
RA Rogers Y.-H., Friedman R., Frazier M., Venter J.C.;
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDP61702.1}.
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DR EMBL; ABHC01000028; EDP61702.1; -; Genomic_DNA.
DR AlphaFoldDB; A8U1Y1; -.
DR STRING; 331869.BAL199_00325; -.
DR eggNOG; COG1032; Bacteria.
DR Proteomes; UP000003417; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000003417}.
FT DOMAIN 28..161
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 210..441
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 675 AA; 74438 MW; 082BB6507D986130 CRC64;
MAEKRLMQNG KPSGEHDLAE KRLVQFVDFP ACNADVPLTL PYATSSLRAY AESFADIRAA
FDFAPTIVLR DRFEAMAERI GRPDLLALSC YVWNFNASMA LAAEVKRRSP QTIVVIGGPH
PKYGDDTVFA AHPAVDALIQ GEGEVTFLAV LRSIAATGRL AAVDGITVRD PDDGTIRVSQ
HRAKAGDLSA MPSPYTIGLM DGPLVDLRGQ SVGVAATLES NRGCPFQCTF CDWGSLGSKM
AEFPIERVFA DIDWIAENGI ETLWMADSNF GMRKRDRDIA IYLAEAHLRT GYPRRLIAST
SKNSSRAVLE VVRPLAQTPM FRGLTLSFQS HSDSALRGIK RQNIKHSTFF ELLEDARSSG
TSTYTDMILG LPEETLDSFK AGLTIAIEGD PQGVLIVFRC VLLPNAELAS AEDRKRFGLR
TAWLPMTDPP DLDPRYTETY EFVVGTDAMP EQDWREAVEY AWLVLLLHTF RLGQPVLLLL
RQVVGLDFVD IFDSMLGFLR RRPDSVLGAQ LQLVQHATAR FIACRDPSNL PPTDQVPWRA
DPDAAARITR AKLAVHHDQC AAELTMLAVA VAKAFGHTIE TEVRDDLFVM LQQTTYRPVA
AGERVLDFAT DLPGVLAAAA AGEKRSITHR PSRYRLMCTE TVSAAGFQAR INDYADRCDG
LEPFDLCLEA ERLAG
//
