ID A8YVB0_LACH4 Unreviewed; 438 AA.
AC A8YVB0;
DT 15-JAN-2008, integrated into UniProtKB/TrEMBL.
DT 15-JAN-2008, sequence version 1.
DT 27-MAR-2024, entry version 67.
DE SubName: Full=Transposase {ECO:0000313|EMBL:ABX27197.1};
GN OrderedLocusNames=lhv_1151 {ECO:0000313|EMBL:ABX27197.1};
OS Lactobacillus helveticus (strain DPC 4571).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=405566 {ECO:0000313|EMBL:ABX27197.1, ECO:0000313|Proteomes:UP000000790};
RN [1] {ECO:0000313|EMBL:ABX27197.1, ECO:0000313|Proteomes:UP000000790}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DPC 4571 {ECO:0000313|EMBL:ABX27197.1,
RC ECO:0000313|Proteomes:UP000000790};
RX PubMed=17993529; DOI=10.1128/JB.01295-07;
RA Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K.,
RA McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F.,
RA Beresford T., Ross R.P.;
RT "Genome sequence of Lactobacillus helveticus: an organism distinguished by
RT selective gene loss and IS element expansion.";
RL J. Bacteriol. 190:727-735(2008).
CC -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC family. {ECO:0000256|ARBA:ARBA00008761}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the transposase 2
CC family. {ECO:0000256|ARBA:ARBA00011044}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000517; ABX27197.1; -; Genomic_DNA.
DR AlphaFoldDB; A8YVB0; -.
DR KEGG; lhe:lhv_1151; -.
DR eggNOG; COG0675; Bacteria.
DR HOGENOM; CLU_032903_0_0_9; -.
DR Proteomes; UP000000790; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR InterPro; IPR010095; Cas12f1-like_TNB.
DR InterPro; IPR001959; Transposase.
DR InterPro; IPR021027; Transposase_put_HTH.
DR NCBIfam; NF040570; guided_TnpB; 1.
DR NCBIfam; TIGR01766; IS200/IS605 family accessory protein TnpB-like domain; 1.
DR PANTHER; PTHR30405:SF20; RNA-GUIDED DNA ENDONUCLEASE INSQ-RELATED; 1.
DR PANTHER; PTHR30405; TRANSPOSASE; 1.
DR Pfam; PF12323; HTH_OrfB_IS605; 1.
DR Pfam; PF01385; OrfB_IS605; 1.
DR Pfam; PF07282; OrfB_Zn_ribbon; 1.
PE 3: Inferred from homology;
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Transposition {ECO:0000256|ARBA:ARBA00022578};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT TRANSMEM 12..31
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 43..87
FT /note="Transposase putative helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12323"
FT DOMAIN 208..342
FT /note="Probable transposase IS891/IS1136/IS1341"
FT /evidence="ECO:0000259|Pfam:PF01385"
FT DOMAIN 354..423
FT /note="Cas12f1-like TNB"
FT /evidence="ECO:0000259|Pfam:PF07282"
SQ SEQUENCE 438 AA; 51049 MW; A9D035690CC7059C CRC64;
MPEKTRDFNP VMNPAFLVFL LVPPFFLKHM FDIILKRKEG TNVLKGIKLR LYPNRTQQNQ
LEQMFGNDRF VWNQMLAMMN ERYQNNKALP FLGKFKLNYL LKPLKKEYPF LKNSDSSSLQ
VVNEFLTQSW KNFFQDKTGQ IGKPRFHSRK YLKKSYTGKS IIKTAGKRYL KIPKLGYVKT
SKTGVLQNTK IKRYTVVLEP TGKYYLSLQV EISEPEKYSL TGKQVGIDVG IADLAILSNG
LKYPSFNSSY FEKKAKIWQK KYSRRRHLVK LLVLQDRNKR VLCPRSLESF TNWQKAQKSK
AKYQAKAANQ RRDYLHKLTT HLVKQYDVIA IEDLKTKNLQ KNHHLAKSIA NASWRMFRQM
LEYKCEWYGK KLIAVDPKNT SRICSKCGYN SGAKPLEIRE WTCSKCQTKH DRDINAAVNI
LHKGSTKLTG QGLAMVTS
//
