ID DDL_DELAS Reviewed; 326 AA.
AC A9BUK8;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 01-MAY-2013, entry version 46.
DE RecName: Full=D-alanine--D-alanine ligase;
DE EC=6.3.2.4;
DE AltName: Full=D-Ala-D-Ala ligase;
DE AltName: Full=D-alanylalanine synthetase;
GN Name=ddl; OrderedLocusNames=Daci_1472;
OS Delftia acidovorans (strain DSM 14801 / SPH-1).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Delftia.
OX NCBI_TaxID=398578;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14801 / SPH-1;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T.,
RA Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D.,
RA Richardson P.;
RT "Complete sequence of Delftia acidovorans DSM 14801 / SPH-1.";
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cell wall formation (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-
CC alanyl-D-alanine.
CC -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC similarity).
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR EMBL; CP000884; ABX34116.1; -; Genomic_DNA.
DR RefSeq; YP_001562501.1; NC_010002.1.
DR ProteinModelPortal; A9BUK8; -.
DR STRING; 398578.Daci_1472; -.
DR EnsemblBacteria; ABX34116; ABX34116; Daci_1472.
DR GeneID; 5747028; -.
DR KEGG; dac:Daci_1472; -.
DR PATRIC; 21636833; VBIDelAci41351_1503.
DR eggNOG; COG1181; -.
DR HOGENOM; HOG000011592; -.
DR KO; K01921; -.
DR OMA; IMIRASD; -.
DR ProtClustDB; PRK01372; -.
DR BioCyc; DACI398578:GHK3-1570-MONOMER; -.
DR UniPathway; UPA00219; -.
DR GO; GO:0005618; C:cell wall; IEA:InterPro.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.30.470.20; -; 2.
DR Gene3D; 3.40.50.20; -; 1.
DR HAMAP; MF_00047; Dala_Dala_lig; 1; -.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR000291; D-Ala_lig_Van_CS.
DR InterPro; IPR005905; D_ala_D_ala.
DR InterPro; IPR011095; Dala_Dala_lig_C.
DR InterPro; IPR011127; Dala_Dala_lig_N.
DR InterPro; IPR016185; PreATP-grasp_dom.
DR PANTHER; PTHR23132; PTHR23132; 1.
DR Pfam; PF07478; Dala_Dala_lig_C; 1.
DR Pfam; PF01820; Dala_Dala_lig_N; 2.
DR SUPFAM; SSF52440; PreATP-grasp-like; 1.
DR TIGRFAMs; TIGR01205; D_ala_D_alaTIGR; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell shape; Cell wall biogenesis/degradation;
KW Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese;
KW Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.
FT CHAIN 1 326 D-alanine--D-alanine ligase.
FT /FTId=PRO_1000091180.
FT DOMAIN 112 312 ATP-grasp.
FT NP_BIND 138 193 ATP (By similarity).
FT METAL 265 265 Magnesium or manganese 1 (By similarity).
FT METAL 279 279 Magnesium or manganese 1 (By similarity).
FT METAL 279 279 Magnesium or manganese 2 (By similarity).
FT METAL 281 281 Magnesium or manganese 2 (By similarity).
SQ SEQUENCE 326 AA; 35184 MW; AD9D6B54E5C4370F CRC64;
MSGFGTQIDV KALGKVAVLL GGRSAEREVS LMSGTGVLKA LLSHGVDAHA FDPAERDLGE
LKKEGFDRCF IALHGRYGED GTVQGALELL GIPYTGPGVM ASSIAMDKIM TKRIWRFEGL
PTPDWRLVAS AGETRAALQA LGAPMIVKPS REGSTIGLTK VWTAEECDQA YVLASRYDPE
VLCEEFIEGD ETTCPVLGTG EGAHALPVIR IVAPEGNYDY QNKYFTDDTK YHCPSGLPAE
EEAEIRRIVV RAFRTLGCRG WSRADIMIRA SDRKPFLLEI NTSPGMTGHS LVPMSARAIG
VSYENLCLGI LASAALDAAQ PEQEHA
//
