ID AACP_AGRFC Reviewed; 83 AA.
AC A9CHM9;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE RecName: Full=Aminoacyl carrier protein;
GN OrderedLocusNames=Atu2571; ORFNames=AGR_C_4658;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
RN [3]
RP FUNCTION AS A CARRIER PROTEIN, PHOSPHOPANTETHEINYLATION, PTM, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C58 / ATCC 33970;
RX PubMed=20663952; DOI=10.1073/pnas.1007470107;
RA Mocibob M., Ivic N., Bilokapic S., Maier T., Luic M., Ban N.,
RA Weygand-Durasevic I.;
RT "Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and
RT provide a link between ribosomal and nonribosomal peptide synthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:14585-14590(2010).
RN [4]
RP STRUCTURE BY NMR.
RG Northeast structural genomics consortium (NESG);
RT "Solution structure of a protein (atc2521) of unknown function from
RT Agrobacterium tumefaciens.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: Aminoacyl carrier protein. Can be charged with L-alanine, L-
CC glycine or L-serine, via the formation of a thioester bond between the
CC amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed
CC by the Atu2573 ligase. {ECO:0000269|PubMed:20663952}.
CC -!- INTERACTION:
CC A9CHM9; Q7CWR3: Atu2573; NbExp=3; IntAct=EBI-16043291, EBI-16043272;
CC -!- PTM: 4'-phosphopantetheine is transferred from CoA to a specific serine
CC of the apo-form of this carrier protein.
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DR EMBL; AE007869; AAK88294.1; -; Genomic_DNA.
DR PIR; AB2892; AB2892.
DR PIR; E97667; E97667.
DR RefSeq; NP_355509.1; NC_003062.2.
DR RefSeq; WP_003504852.1; NC_003062.2.
DR PDB; 2JQ4; NMR; -; A=1-83.
DR PDB; 4H2W; X-ray; 1.95 A; C/D=1-83.
DR PDB; 4H2X; X-ray; 2.15 A; C/D=1-83.
DR PDB; 4H2Y; X-ray; 2.10 A; C/D=1-83.
DR PDBsum; 2JQ4; -.
DR PDBsum; 4H2W; -.
DR PDBsum; 4H2X; -.
DR PDBsum; 4H2Y; -.
DR AlphaFoldDB; A9CHM9; -.
DR BMRB; A9CHM9; -.
DR SMR; A9CHM9; -.
DR DIP; DIP-60154N; -.
DR IntAct; A9CHM9; 1.
DR STRING; 176299.Atu2571; -.
DR EnsemblBacteria; AAK88294; AAK88294; Atu2571.
DR GeneID; 66222760; -.
DR KEGG; atu:Atu2571; -.
DR PATRIC; fig|176299.10.peg.2575; -.
DR eggNOG; COG0236; Bacteria.
DR HOGENOM; CLU_108696_10_0_5; -.
DR OrthoDB; 7284767at2; -.
DR BioCyc; AGRO:ATU2571-MONOMER; -.
DR EvolutionaryTrace; A9CHM9; -.
DR Proteomes; UP000000813; Chromosome circular.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR Pfam; PF00550; PP-binding; 1.
DR SUPFAM; SSF47336; ACP-like; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Phosphopantetheine; Phosphoprotein; Reference proteome.
FT CHAIN 1..83
FT /note="Aminoacyl carrier protein"
FT /id="PRO_0000401191"
FT DOMAIN 1..80
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 35
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT HELIX 1..12
FT /evidence="ECO:0007829|PDB:4H2W"
FT HELIX 19..21
FT /evidence="ECO:0007829|PDB:4H2W"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:4H2W"
FT HELIX 28..31
FT /evidence="ECO:0007829|PDB:4H2W"
FT HELIX 35..48
FT /evidence="ECO:0007829|PDB:4H2W"
FT HELIX 55..57
FT /evidence="ECO:0007829|PDB:4H2Y"
FT HELIX 60..63
FT /evidence="ECO:0007829|PDB:4H2W"
FT HELIX 66..74
FT /evidence="ECO:0007829|PDB:4H2W"
FT HELIX 79..82
FT /evidence="ECO:0007829|PDB:2JQ4"
SQ SEQUENCE 83 AA; 8987 MW; E4A796F291C8036E CRC64;
MNATIREILA KFGQLPTPVD TIADEADLYA AGLSSFASVQ LMLGIEEAFD IEFPDNLLNR
KSFASIKAIE DTVKLILDGK EAA
//
