ID A9EAB4_9FLAO Unreviewed; 3274 AA.
AC A9EAB4;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 92.
DE SubName: Full=Polyketide synthase of type I {ECO:0000313|EMBL:EDP94609.1};
GN ORFNames=KAOT1_04310 {ECO:0000313|EMBL:EDP94609.1};
OS Kordia algicida OT-1.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Kordia.
OX NCBI_TaxID=391587 {ECO:0000313|EMBL:EDP94609.1, ECO:0000313|Proteomes:UP000002945};
RN [1] {ECO:0000313|EMBL:EDP94609.1, ECO:0000313|Proteomes:UP000002945}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OT-1 {ECO:0000313|EMBL:EDP94609.1,
RC ECO:0000313|Proteomes:UP000002945};
RX PubMed=21622754; DOI=10.1128/JB.05241-11;
RA Lee H.S., Kang S.G., Kwon K.K., Lee J.H., Kim S.J.;
RT "Genome sequence of the algicidal bacterium Kordia algicida OT-1.";
RL J. Bacteriol. 193:4031-4032(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000256|RuleBase:RU003707}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDP94609.1}.
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DR EMBL; ABIB01000015; EDP94609.1; -; Genomic_DNA.
DR STRING; 391587.KAOT1_04310; -.
DR eggNOG; COG3321; Bacteria.
DR HOGENOM; CLU_000022_53_1_10; -.
DR Proteomes; UP000002945; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd06558; crotonase-like; 2.
DR CDD; cd08953; KR_2_SDR_x; 1.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 1.10.1240.100; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 6.20.390.20; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00378; ECH_1; 2.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52096; ClpP/crotonase; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000002945};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 475..551
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 611..1031
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2314..2388
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2423..2500
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2561..2984
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT COILED 244..292
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 3274 AA; 364748 MW; C90C3ABF832BE2AE CRC64;
MMKEQLKKIF LDLSSKKLSE KEALEKIRAI KAAQKHSDVS TLYASPTWKI VKNPHDNQAV
NAHHVICLQI ADNAQKVLAK KLPQSTIHTV KASNFSEIAL SCFSIIKNIL ENKIQANSYV
QIVLGTSVEE TLLQSLSALF KTATLENHNL IGQLIVSDQK NAVEKLAAQL IEETQQSTDA
IIKYEGGKRA VKRLEEIQTQ QSNNSAYKEN GVYLITGGFG GVGIAFAKEI LQQLEKTTII
LTGRSKLTAE KRQLLQQLSE KNKKVQYIQM DVANEQKVAE NVQNILKEYK KLHGIIHAAG
INKDNFILKK SVAEFKEVLQ AKVAGTQNID EATKDVDLDF ITYFSSIAAW FGNPGQADYA
VANAFMDEFA KQRNQLVQAG KRKGKTLSIN WPLWENGGME IDSEIKARLL EETGFIPLDD
QTGTTIFQKA INTNQTHILG VKGSLEKIKD TLFNQVVKVQ QQEEEIISET LDEKELLRKT
TAFVRDEFST IIKLPAREIE THVALEKYGI DSILAMNITS QLEKTFGRLS KTLFFEYQTI
DELSEYLANA FTSVLTAKFS SNATKKQSVS NTKKTIVHSK SHQTKTTKKK IRTQSEQSFN
VQFTPQNTNQ NEPIAIVGLS GRYPESENIQ EFWNNLQQGK DCIIEVPKSR WDWKKHYSKD
RTEQNRHYSK WGGFIAGVDE FDPRFFNISP REAKNIDPQE RLFLQHSWMA MEDAGYTREA
LHKASENNLP GQVGVYVGVM YGEYNLSGSL ASIANRVSYV LNLHGPSITL DTMCSSSLTS
VHLACQDLKS GRTDMALAGG VNVSIDANKY QMLSTGQFIS SDGHCQSFGE GGDGYIPGEG
VGVVMLKRLS DAEKDGDHIY GIIKGSALNH GGKTNGYSVP NPKAQASVIS RALREANVHP
RHVSYIEAHG TGTKLGDPIE ITALNKAFGI DNENHFCQLG SAKSNIGHCE SAAGIAGITK
VILQLQHKQI VPSLHSAKLN PHIDFEHTPF VVNQSLKTWE NPIVDGNIIP RIAGVSSFGA
GGANAHILIE EYNSVKNVAT HTPNTEVAIV LSARTKPQLK QKIQELARFI ENQEHTPDLL
AIAYTLQTGR EAMDERFATI VFSIEELTKT LNAYLGNALQ TSKYHQARTK DHKNDVLDFK
SEANFDETCA TWLQNKNYGE LLAWWTKGID VQWNELYATQ KPQRISLPTY PFAKESYLLT
PEERGRATKE TTKAIIHPLV HENTSNISET RFSSTFNGEE AYIKDFTIHK ENQTLHGLPF
MASLEMAREA IAQAIPQQKE DNVLSITNVQ FGAFMQASSV NQIHIALLPE SESVASYEIY
SQHDDEDIIY TQGKARYTDK KATLQVDINA LRQQMSKAVI SGTTIYENAV QYGTSYRGIK
TVHFGNQQIL AHLQLPSEVV NDVKSYVLHP TILESAVKAV SGIAGNQNEI SATTIIPTEI
TSVNIHASCT SEMFAWIRYS ENDSQAENEV KIDIDLCDTN GTICAQFVGI SFSTSVESTQ
FEPTLETKET SVKAAATATL AETFETPKEL FFTVQTKPTI ALTATNAENT VSSLEKPSSI
KLTKPTAIVV DKKNSATLKK GRVQLSVESS QQIIETTVPM LRLFNTGNGI YKLQLETSEL
DKTFIVQFKS ALQRLAKEEN LKVLLIEGTQ TDFLHGESEV FNEALQQELF KEILSFNYPI
IANVQGNASN VGFLLASLCD FIICNEQATY EFSNTNDKLT NLFNERFGAV LANNLLQPNK
SFTGTQLKQN GFTMPIVASE KVTKTAADLA DNLAKKSQRT LRLLKQHLSR HISEKVEQLT
AISTITENTA FEKVEISETK SFKVQYQKDG VCVVTIKNTK LKGIRTLNSE IGAFFSEVNN
TKNHSTIVLK SEQESFIPQT AKDVEIIKLS EILLNCNYPI ITELQNSPNA KAWLVSLHTD
EVIYSETGNY TAKELLANKT LAQKATLLFT ACYGELASKE LLLLGKSYNG KELKTVIPTI
QTATNATELA KSWSTAKNTK TWKSAKIKAI KESKKELPTW KLATAKDEKA PKTGTVNLKS
SVVKATVLEA GILEIRLEER GSKNMFTDDF MAGVNEVFEH IENTPNYKVV ILTGYDNYFA
SGGTKEGLVA IQEGKSKFTD TKIFQLAMEC KIPVIAAMQG HAIGAGWAMG MYADFTLFSK
ERKYLSPYMN YGFTPGAGAT YIFPEIMGYD LARETLFTAK EYSGLDFENR GVALPVLTKD
EVVTYAVELA KMIAKNSRNT LIAFKQQLIQ NRVAEAEATK KLELAMHDET FVGDAATLQQ
IQENFAYKQA TTNIDEVITT EKEVSNDIEE NTISDVVETI KKFLANELQL EENEIEEDAQ
FVDIGLDSIT GVTWIRKIND YYKLSLEATQ IYTYTTLQQL GDFIQKELGG TIRTTKETKT
VATPAPKEVE TIVYEEEVIE TVDGTAEIVE TIKKFLANEL QLEENEIEED AQFIDIGLDS
ITGVTWIRKI NDHYKTSIEA TQIYTYPSLN SLSEFIKEEL QRLGLTETKK ITKKVVATTP
KTVQTIPNSN NTFQFTERKL TSIRSKKAIK KSVAKTNNYT SQAIAVVGMA GQFPKAENVQ
QFWQNIAQGE NCITEVPKER WDINEYYSEG TPTPGKTNSK WLGSLAGYDQ FDPLFFTISP
IEAESMDPQQ RLFLQSCWHS IEDAGYNPQA LSGTKCGVFV GCATGDYQLP SREQQLSAQG
FTGGTSSILA ARISYFLNLQ GPCIALDTAC SSSLVAIANA CDSLASGASD SALAGGVYVM
TGPEMHVKTA QSGMLSKDGK CHTFDQEANG FVPGEAVGVV MLKRLEDAER DNDNIYGVIK
GWGLNQDGRT NGITAPNSQS QTNLEQEVYD RYDINPEEIQ LIEAHGTGTK LGDPIEVAAL
KNSFKKYTEK EQYCALGSVK SNIGHCLTAA GVAGFIKVMK AMEHKKLPPT IHYNQLNEHI
SLEKSPFYVN DRLQDWEVGQ AEIRHAAISA FGFSGTNAHL VMSEYIPETV DKSQINVITQ
NKKYLIPISA RNEEQLEIQA ENLLAYAKQN LTTIDLVEMA YTLQVGRDAM EARVGFMVTS
VEELIDKVEA YLQGEEFIKD FYQGHIKENK EGLKLVSGDK EMKELIIQKW VSDKKLNKLL
DLWTKGLDFD WNILYGNQKP KRQRLPIYPF AKESYWLEAT TINYNAEIPE EKSAKLHPLV
HEKTEDLKAQ GLTDATQKDA TTETKRKKIK RINLPTYPFD QEKCWPETAP KAVETPKEST
IVFKDLDAIE DVIERIDGEL LDTDEAVLIL KDLL
//
