ID A9US44_MONBE Unreviewed; 1322 AA.
AC A9US44;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN ORFNames=MONBRDRAFT_19544 {ECO:0000313|EMBL:EDQ91715.1};
OS Monosiga brevicollis (Choanoflagellate).
OC Eukaryota; Choanoflagellata; Craspedida; Salpingoecidae; Monosiga.
OX NCBI_TaxID=81824 {ECO:0000313|EMBL:EDQ91715.1, ECO:0000313|Proteomes:UP000001357};
RN [1] {ECO:0000313|EMBL:EDQ91715.1, ECO:0000313|Proteomes:UP000001357}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MX1 / ATCC 50154 {ECO:0000313|Proteomes:UP000001357};
RX PubMed=18273011; DOI=10.1038/nature06617;
RG JGI Sequencing;
RA King N., Westbrook M.J., Young S.L., Kuo A., Abedin M., Chapman J.,
RA Fairclough S., Hellsten U., Isogai Y., Letunic I., Marr M., Pincus D.,
RA Putnam N., Rokas A., Wright K.J., Zuzow R., Dirks W., Good M.,
RA Goodstein D., Lemons D., Li W., Lyons J.B., Morris A., Nichols S.,
RA Richter D.J., Salamov A., Bork P., Lim W.A., Manning G., Miller W.T.,
RA McGinnis W., Shapiro H., Tjian R., Grigoriev I.V., Rokhsar D.;
RT "The genome of the choanoflagellate Monosiga brevicollis and the origin of
RT metazoans.";
RL Nature 451:783-788(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC PRP16 sub-subfamily. {ECO:0000256|ARBA:ARBA00038040}.
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DR EMBL; CH991544; EDQ91715.1; -; Genomic_DNA.
DR RefSeq; XP_001743001.1; XM_001742949.1.
DR STRING; 81824.A9US44; -.
DR EnsemblProtists; EDQ91715; EDQ91715; MONBRDRAFT_19544.
DR GeneID; 5888779; -.
DR KEGG; mbr:MONBRDRAFT_19544; -.
DR eggNOG; KOG0924; Eukaryota.
DR InParanoid; A9US44; -.
DR OMA; VDVMFHR; -.
DR Proteomes; UP000001357; Unassembled WGS sequence.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0034458; F:3'-5' RNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF91; PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000001357}.
FT DOMAIN 620..804
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 818..1000
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 74..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 133..378
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 557..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1279..1322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..150
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..332
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..364
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..576
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1282..1296
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1322 AA; 149638 MW; 9388984DC7881A01 CRC64;
MADDLFLEEV AVKISAALNL SLNNRGLARQ VVDAGRAAPS LERFVKTCSA FGRFESQLLE
DLYCRIKTVK GSSAAAGAGG AAPEHAQQLP ASEPEQGGLF VRRPRNIKPA EGAEDLVFRP
PKSVLGLDRL AAAKRRQKAE QEKNAEGAES KRSKPTSGVH LGWEAEEEAE PVGSAATRPS
QDGDDIKLKS RSSRPKAGRR RHPGEATPSE GAGLATPLRE ELHQRHRDQR FGADRPRDDR
RHNDRDEHWS RYDDRRGRDR RPYDDRRSRD ERDRRYDDQG REGRRDAGSW ERRGSHEHER
ERSRDDRTPR SDRRLQTPGR RYSSDSHVRI SRGRRRTTGG STPASMYRST PISTPSYKRN
SWNQSESQKH GDRVYREDDH ERPDFANEEE EAAWKHEQDQ IERHWYYEDG HDDDFDPFAG
MADYAAKKEQ QLQLAKPVER INAFKRQRNA DQDRWEQNRM LTSGAVQRVG PMDDDEDDDV
GKVHLLVHHL VPPFLDGRIT FSKQPEPVYP VRDPASDIAV LAKKGSMLVR REREKQDAVK
GQKKEWELAG TALGKIMGVK DKSDDPADQE PEDHAAGSKF AEHMKEDDKE DNATSHFART
KTIKEQRQYL PIYAVRQELL NIIRDNQVII IVGQTGSGKT TQMTQYLYEA GYGDFGTIGC
TQPRRVAAMS VAKRVSEEMG VELGKQVGYS IRFEDVTSRE TVIKYMTDGI LLRESLNEGD
LDQYSAIIMD EAHERSLNTD VLFGLLRDVV ARCGTAAPRC VRPSHSRVPR CIRRRDLKLI
VTSATMDSTK FATFFGNVPV FNIPGRTFPV EVFFAKNPVD DYVEAAIKQA VQIHLQPHPG
DMLIFMTGQA DIEATCSVLA ERLEALGEDV PPLSILPIYS QLPSDLQAKI FKKSDVRKCI
VATNIAETSL TVDGIMHVID SGFCKLKCYN PKIGIDDLQI YPISQANANQ RSGRAGRTGP
GNAYRLYTEA IYKNELLPLT VPEIQRTNLA NVVLLLKSLG VENLMDFHFM DPPPQETILN
SMYNLWILGA LDNTGALTPL GRQMVEFPLD PAQSKMLIVS ADLECSSEIL TIVSMLSVDK
HFFRPPGREE ESDLKREKFA VPESDHLTLL NTYQQWKSNN YSSSWASEHF IHAKSMRKVR
EIRMQLMDIM KSQKVPVISS GTSWDAVRKC ICSAYFHHAA RLKGIGEYVN ARTGMPAHLH
PTSSLYGMGI NPDWVCYHDL VMTTKEYMQF VTAVDPLWLA ELGNIFFSVK DSAEARLRRK
KENDQESKHM EQEMTEALNA MRARDASREQ ERKALSRSIR IATPGSRREP GTPRSTPRRF
GL
//
