ID A9V8U8_MONBE Unreviewed; 1167 AA.
AC A9V8U8;
DT 05-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 76.
DE RecName: Full=DNA-directed RNA polymerase {ECO:0008006|Google:ProtNLM};
GN ORFNames=MONBRDRAFT_33973 {ECO:0000313|EMBL:EDQ86023.1};
OS Monosiga brevicollis (Choanoflagellate).
OC Eukaryota; Choanoflagellata; Craspedida; Salpingoecidae; Monosiga.
OX NCBI_TaxID=81824 {ECO:0000313|EMBL:EDQ86023.1, ECO:0000313|Proteomes:UP000001357};
RN [1] {ECO:0000313|EMBL:EDQ86023.1, ECO:0000313|Proteomes:UP000001357}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MX1 / ATCC 50154 {ECO:0000313|Proteomes:UP000001357};
RX PubMed=18273011; DOI=10.1038/nature06617;
RG JGI Sequencing;
RA King N., Westbrook M.J., Young S.L., Kuo A., Abedin M., Chapman J.,
RA Fairclough S., Hellsten U., Isogai Y., Letunic I., Marr M., Pincus D.,
RA Putnam N., Rokas A., Wright K.J., Zuzow R., Dirks W., Good M.,
RA Goodstein D., Lemons D., Li W., Lyons J.B., Morris A., Nichols S.,
RA Richter D.J., Salamov A., Bork P., Lim W.A., Manning G., Miller W.T.,
RA McGinnis W., Shapiro H., Tjian R., Grigoriev I.V., Rokhsar D.;
RT "The genome of the choanoflagellate Monosiga brevicollis and the origin of
RT metazoans.";
RL Nature 451:783-788(2008).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit
CC family. {ECO:0000256|ARBA:ARBA00009307}.
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DR EMBL; CH991569; EDQ86023.1; -; Genomic_DNA.
DR RefSeq; XP_001749217.1; XM_001749165.1.
DR AlphaFoldDB; A9V8U8; -.
DR STRING; 81824.A9V8U8; -.
DR ESTHER; monbe-a9v8u8; 6_AlphaBeta_hydrolase.
DR EnsemblProtists; EDQ86023; EDQ86023; MONBRDRAFT_33973.
DR GeneID; 5894496; -.
DR KEGG; mbr:MONBRDRAFT_33973; -.
DR eggNOG; KOG0631; Eukaryota.
DR eggNOG; KOG3297; Eukaryota.
DR InParanoid; A9V8U8; -.
DR Proteomes; UP000001357; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005666; C:RNA polymerase III complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006384; P:transcription initiation at RNA polymerase III promoter; IBA:GO_Central.
DR CDD; cd04330; RNAP_III_Rpc25_N; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.30.70.890; GHMP kinase, C-terminal domain; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.30.1490.120; RNA polymerase Rpb7-like, N-terminal domain; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR013238; RNA_pol_III_Rbc25.
DR InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR InterPro; IPR045113; Rpb7-like.
DR InterPro; IPR005576; Rpb7-like_N.
DR PANTHER; PTHR12709; DNA-DIRECTED RNA POLYMERASE II, III; 1.
DR PANTHER; PTHR12709:SF1; DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR Pfam; PF08292; RNA_pol_Rbc25; 1.
DR Pfam; PF03876; SHS2_Rpb7-N; 1.
DR PRINTS; PR00959; MEVGALKINASE.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF55060; GHMP Kinase, C-terminal domain; 1.
DR SUPFAM; SSF88798; N-terminal, heterodimerisation domain of RBP7 (RpoE); 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000001357};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 214..236
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 826..845
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1011..1037
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1049..1068
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 8..64
FT /note="RNA polymerase Rpb7-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03876"
FT DOMAIN 83..190
FT /note="RNA polymerase III subunit Rpc25"
FT /evidence="ECO:0000259|Pfam:PF08292"
FT DOMAIN 409..477
FT /note="GHMP kinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00288"
SQ SEQUENCE 1167 AA; 128632 MW; 86B92E3FFFED3F11 CRC64;
MFRVATLKDI VQLKPKRFGE NRVDALTHEI NKKYANKVIY KLGLAICLFD IVEIKEAHVF
PGDGSAHFYV QFRLVVFRPF TGEVLKGAIE SSTADHIKVS LKFFRDIYIP AHAMREGVVF
DDGLQEWIWQ YQDEDGASHQ LKLAKGCEIT FKVMEETFHD VIPVSEHKKQ TTPDGLMVKK
IEELPYVIMG PVAHPHKNIQ LMIQSKKLSA VPPVSLSLSL SLSLLHVVVV LFPLLFNSRS
LSISLSLSLS LSLSLFCLYE FLIQGQLVSL LCAPRLPTHT ISMGEAETSA RKILTALANE
HGLDVKGGKV CRTSSRFCLG VEHGDYNGTE LFGVGTDRFI WVAFKPNGTK QIRLASHNFP
ADGLVEFEID KYPSKDDPSI QSSWSRFPYG TNSVLTAHGY KLTQGFDAVV YGNIPGGGMS
RSASLCINLL LTMADVNGIR FGTTPEQQMA VVDMAQEIEN DYIGSPCGKL DQIMIFFAKE
GQGTHYKPAT RSISYVPLGA KAEQFALVSL DTGTERLGLE KSTYKIRRQE CDELAAMGKA
AFGIETLADV KDEDMLNVGF SVPFLAQGLG AISAKKMRAR FGTEFPNHIK RLEYIYAAQQ
RFYEMMDAWR AGDISKVGAI FREDGYGLRD VYEISGPELE TMCDIARTVE GVLGERMLGG
GDKGASGALV LSSAVEELRE AVDREYPKRQ PEYANKYKTH ACRVVDGVQM QPGFSPIRIE
SCVSSGNLFF ITKHTHTHTH SLSLKISLKI SLKISLKISL FQALSLSLSL SLSLSLSLSL
SLKLFLFLSL SQTLKLSLSN SQTLSLSPKL SLKLSLSSSL SQALKLSLSL IFFFISLSLS
LSLSLSSKAR WACWSTGKSC AQSIFCKTRL PSRFVSQLPT ACYWTAFTCR LQGGPRIGKQ
LEHYSRALNV HVIAFNYRGV GDSQGWPFVA ADLCKDGEAA VKYAMDLGAT ESNLILYGHS
LGGGVVGELS TIFPSALRVY DRTFSGPVTV LALPSTMRGH APNKARTLPT VAAHFMSMLG
PFMGCALGVV AALGYHMTTG ALFPEYQHMR WAVLAGLVGG YVLGKTALMG PLAANMLKFA
YWDLGANTRW DPDRSFCIFH RHDEVINFRA SSIYPGLEAQ FGPNLRSVEL FTRGNNIMSH
MYSLPDAEHE FAQVTATITS MLRALQQ
//
