ID B0BL08_ECOLX Unreviewed; 157 AA.
AC B0BL08;
DT 26-FEB-2008, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2008, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=dihydrofolate reductase {ECO:0000256|ARBA:ARBA00012856};
DE EC=1.5.1.3 {ECO:0000256|ARBA:ARBA00012856};
GN Name=dfrA17 {ECO:0000313|EMBL:CAP69686.1};
OS Escherichia coli.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562 {ECO:0000313|EMBL:CAP69686.1};
RN [1] {ECO:0000313|EMBL:CAP69686.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=854 {ECO:0000313|EMBL:CAP69686.1};
RX PubMed=18550679; DOI=10.1093/jac/dkn233;
RA Kadlec K., Schwarz S.;
RT "Analysis and distribution of class 1 and class 2 integrons and associated
RT gene cassettes among Escherichia coli isolates from swine, horses, cats and
RT dogs collected in the BfT-GermVet monitoring study.";
RL J. Antimicrob. Chemother. 62:469-473(2008).
RN [2] {ECO:0000313|EMBL:CAP69686.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=854 {ECO:0000313|EMBL:CAP69686.1};
RA Schwarz S.P.;
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Key enzyme in folate metabolism. Catalyzes an essential
CC reaction for de novo glycine and purine synthesis, and for DNA
CC precursor synthesis. {ECO:0000256|ARBA:ARBA00025067}.
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-
CC tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
CC {ECO:0000256|ARBA:ARBA00004903}.
CC -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00009539, ECO:0000256|RuleBase:RU004474}.
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DR EMBL; AM932673; CAP69686.1; -; Genomic_DNA.
DR RefSeq; WP_063844367.1; NG_047711.1.
DR AlphaFoldDB; B0BL08; -.
DR SMR; B0BL08; -.
DR BindingDB; B0BL08; -.
DR DrugCentral; B0BL08; -.
DR UniPathway; UPA00077; UER00158.
DR GO; GO:0004146; F:dihydrofolate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00209; DHFR; 1.
DR Gene3D; 3.40.430.10; Dihydrofolate Reductase, subunit A; 1.
DR InterPro; IPR012259; DHFR.
DR InterPro; IPR024072; DHFR-like_dom_sf.
DR InterPro; IPR017925; DHFR_CS.
DR InterPro; IPR001796; DHFR_dom.
DR PANTHER; PTHR48069; DIHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR48069:SF3; DIHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF00186; DHFR_1; 1.
DR PRINTS; PR00070; DHFR.
DR SUPFAM; SSF53597; Dihydrofolate reductase-like; 1.
DR PROSITE; PS00075; DHFR_1; 1.
DR PROSITE; PS51330; DHFR_2; 1.
PE 3: Inferred from homology;
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 2..156
FT /note="DHFR"
FT /evidence="ECO:0000259|PROSITE:PS51330"
SQ SEQUENCE 157 AA; 17532 MW; 2C80D7CB0CF90519 CRC64;
MKISLISAVS ESGVIGSGPD IPWSVKGEQL LFKALTYNQW LLVGRKTFDS MGVLPNRKYA
VVSKNGISSS NENVLVFPSI ENALKELSKV TDHVYVSGGG QIYNSLIEKA DIIHLSTVHV
EVEGDIKFPI MPENFNLVFE QFFMSNINYT YQIWKKG
//
