ID MKAR_LACBS Reviewed; 338 AA.
AC B0D8R3;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 1.
DT 03-APR-2013, entry version 34.
DE RecName: Full=Very-long-chain 3-oxoacyl-CoA reductase;
DE EC=1.1.1.330;
DE AltName: Full=3-ketoacyl-CoA reductase;
DE Short=3-ketoreductase;
DE Short=KAR;
DE AltName: Full=Microsomal beta-keto-reductase;
GN ORFNames=LACBIDRAFT_192627;
OS Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured
OS deceiver) (Laccaria laccata var. bicolor).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
OC Agaricomycetes; Agaricomycetidae; Agaricales; Tricholomataceae;
OC Laccaria.
OX NCBI_TaxID=486041;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S238N-H82 / ATCC MYA-4686;
RX PubMed=18322534; DOI=10.1038/nature06556;
RA Martin F., Aerts A., Ahren D., Brun A., Danchin E.G.J., Duchaussoy F.,
RA Gibon J., Kohler A., Lindquist E., Pereda V., Salamov A.,
RA Shapiro H.J., Wuyts J., Blaudez D., Buee M., Brokstein P.,
RA Canbaeck B., Cohen D., Courty P.E., Coutinho P.M., Delaruelle C.,
RA Detter J.C., Deveau A., DiFazio S., Duplessis S.,
RA Fraissinet-Tachet L., Lucic E., Frey-Klett P., Fourrey C.,
RA Feussner I., Gay G., Grimwood J., Hoegger P.J., Jain P., Kilaru S.,
RA Labbe J., Lin Y.C., Legue V., Le Tacon F., Marmeisse R., Melayah D.,
RA Montanini B., Muratet M., Nehls U., Niculita-Hirzel H.,
RA Oudot-Le Secq M.P., Peter M., Quesneville H., Rajashekar B., Reich M.,
RA Rouhier N., Schmutz J., Yin T., Chalot M., Henrissat B., Kuees U.,
RA Lucas S., Van de Peer Y., Podila G.K., Polle A., Pukkila P.J.,
RA Richardson P.M., Rouze P., Sanders I.R., Stajich J.E., Tunlid A.,
RA Tuskan G., Grigoriev I.V.;
RT "The genome of Laccaria bicolor provides insights into mycorrhizal
RT symbiosis.";
RL Nature 452:88-92(2008).
CC -!- FUNCTION: Component of the microsomal membrane bound fatty acid
CC elongation system, which produces the 26-carbon very long-chain
CC fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the
CC 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty
CC acid elongation. VLCFAs serve as precursors for ceramide and
CC sphingolipids (By similarity).
CC -!- CATALYTIC ACTIVITY: A very-long-chain (3R)-3-hydroxyacyl-CoA +
CC NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane (By
CC similarity); Single-pass membrane protein (Potential).
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC (SDR) family.
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DR EMBL; DS547100; EDR09116.1; -; Genomic_DNA.
DR RefSeq; XP_001880429.1; XM_001880394.1.
DR ProteinModelPortal; B0D8R3; -.
DR STRING; 29883.JGI192627; -.
DR GeneID; 6076163; -.
DR KEGG; lbc:LACBIDRAFT_192627; -.
DR eggNOG; COG0300; -.
DR KO; K10251; -.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.720; -; 1.
DR HAMAP; MF_03107; 3-ketoreductase; 1; -.
DR InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR InterPro; IPR002347; Glc/ribitol_DH.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR Pfam; PF00106; adh_short; 1.
DR PIRSF; PIRSF000126; 11-beta-HSD1; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW Complete proteome; Endoplasmic reticulum; Fatty acid biosynthesis;
KW Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Membrane;
KW NADP; Oxidoreductase; Transmembrane; Transmembrane helix.
FT CHAIN 1 338 Very-long-chain 3-oxoacyl-CoA reductase.
FT /FTId=PRO_0000357311.
FT TRANSMEM 20 40 Helical; (Potential).
FT ACT_SITE 214 214 Proton acceptor (By similarity).
FT BINDING 201 201 Substrate (By similarity).
SQ SEQUENCE 338 AA; 36601 MW; 63859EE86F225A41 CRC64;
MDVFNVQELS FSLVRDQPYL SAFLLVMGSI GVGRVIYQTL SVFLQTFILP GTNLRKFGAK
KGAWAVVTGA TDGIGREFSL QLAKAGFHVF LVARNEALLA STAAEIEQKY GVSTATHSID
FSKADKSAYN SLGSSLGSVD VGVLVNNVGK SHAMPAYFVD TPEEEMSDIV SINVQATLQV
THSVLPGMVQ RKRGLILNVG SFAGAVPSPM LATYSGTKAF LTTFSSALGE EVRKDNITVE
HLNTYFVVSK LSKIRKASAL IPKPDAYVRS VLSKIGLPCG ASYSGRPNTS TPFWSHALLD
YGLTLIGLQS AFISYTHGLH KDIRRRALRK MERDAKLQ
//
