ID B0JK51_MICAN Unreviewed; 529 AA.
AC B0JK51;
DT 18-MAR-2008, integrated into UniProtKB/TrEMBL.
DT 18-MAR-2008, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE SubName: Full=Glycoside hydrolase family 57 {ECO:0000313|EMBL:BAG05905.1};
GN OrderedLocusNames=MAE_60830 {ECO:0000313|EMBL:BAG05905.1};
OS Microcystis aeruginosa (strain NIES-843 / IAM M-2473).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Microcystaceae; Microcystis.
OX NCBI_TaxID=449447 {ECO:0000313|EMBL:BAG05905.1, ECO:0000313|Proteomes:UP000001510};
RN [1] {ECO:0000313|EMBL:BAG05905.1, ECO:0000313|Proteomes:UP000001510}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-843 {ECO:0000313|EMBL:BAG05905.1,
RC ECO:0000313|Proteomes:UP000001510};
RX PubMed=18192279; DOI=10.1093/dnares/dsm026;
RA Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M.,
RA Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A.,
RA Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M.,
RA Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.;
RT "Complete genomic structure of the bloom-forming toxic cyanobacterium
RT Microcystis aeruginosa NIES-843.";
RL DNA Res. 14:247-256(2007).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 57 family.
CC {ECO:0000256|ARBA:ARBA00006821, ECO:0000256|RuleBase:RU361196}.
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DR EMBL; AP009552; BAG05905.1; -; Genomic_DNA.
DR RefSeq; WP_012268235.1; NC_010296.1.
DR AlphaFoldDB; B0JK51; -.
DR STRING; 449447.MAE_60830; -.
DR CAZy; GH57; Glycoside Hydrolase Family 57.
DR PaxDb; 449447-MAE_60830; -.
DR EnsemblBacteria; BAG05905; BAG05905; MAE_60830.
DR GeneID; 84636391; -.
DR KEGG; mar:MAE_60830; -.
DR PATRIC; fig|449447.4.peg.5569; -.
DR eggNOG; COG1543; Bacteria.
DR HOGENOM; CLU_008192_1_0_3; -.
DR BioCyc; MAER449447:MAE_RS26555-MONOMER; -.
DR Proteomes; UP000001510; Chromosome.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0030979; P:alpha-glucan biosynthetic process; IEA:InterPro.
DR CDD; cd10792; GH57N_AmyC_like; 1.
DR Gene3D; 1.20.1430.10; Families 57/38 glycoside transferase, middle domain; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR InterPro; IPR037090; 57_glycoside_trans_central.
DR InterPro; IPR015293; BE_C.
DR InterPro; IPR040042; Branching_enz_MT3115-like.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR004300; Glyco_hydro_57_N.
DR PANTHER; PTHR41695; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR PANTHER; PTHR41695:SF1; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR Pfam; PF09210; BE_C; 1.
DR Pfam; PF03065; Glyco_hydro_57; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361196};
KW Hydrolase {ECO:0000313|EMBL:BAG05905.1}.
FT DOMAIN 7..328
FT /note="Glycoside hydrolase family 57 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03065"
FT DOMAIN 427..526
FT /note="1,4-alpha-glucan branching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF09210"
FT ACT_SITE 189
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT ACT_SITE 353
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT BINDING 241
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 258
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 407
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 466
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
SQ SEQUENCE 529 AA; 62433 MW; F6D60192EDD887BD CRC64;
MAFGYVALVL HAHLPFVRHP ESDYVLEEEW LYEAITETYI PLLQVFEGLK RDGIDFKMTM
SMTPPLVSML RDPLLQERYE EHLGKLEELI AKEIDHNQHN GHIRYLAEYY AESFQSIRQT
WEKYDRDLVK AFKQFLDSNN LEIITCGATH GYMPLMKMYP QAVWAQIEVA CQHYEENFGR
RPKGIWLPEC AYYEGVERML ADAGIRYFLV DGHGILYGRP RPRYGSYAPI FTETGVAAFG
RDHESSQQVW SSQVGYPGDI VYREFYKDLG WEAEYEYIKP YIMPNGQRKN IGIKYHKITS
RDGGLSEKGL YDPYWAKEKA AEHAGNFMYN RERQVESLAG IMGRPPLVVS PYDAELFGHW
WYEGPWFIDF LFRKSWYDQN IYQMTHLADY LRSNPTQQVC RPSQSSWGYK GFHEYWLNET
NAWIYPHLHK GTERMIELSH HEAEDELQER ALNQAARELL LAQSSDWAFI MRTGTMVPYA
VRRTRSHLLR LNKIYEDVKS EKIDAGWLEK VEEIDNIFPN IDYRVYRPL
//
