ID B0KN21_PSEPG Unreviewed; 1102 AA.
AC B0KN21;
DT 18-MAR-2008, integrated into UniProtKB/TrEMBL.
DT 18-MAR-2008, sequence version 1.
DT 27-MAR-2024, entry version 75.
DE SubName: Full=MscS Mechanosensitive ion channel {ECO:0000313|EMBL:ABZ01002.1};
GN OrderedLocusNames=PputGB1_5117 {ECO:0000313|EMBL:ABZ01002.1};
OS Pseudomonas putida (strain GB-1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=76869 {ECO:0000313|EMBL:ABZ01002.1, ECO:0000313|Proteomes:UP000002157};
RN [1] {ECO:0000313|EMBL:ABZ01002.1, ECO:0000313|Proteomes:UP000002157}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GB-1 {ECO:0000313|EMBL:ABZ01002.1,
RC ECO:0000313|Proteomes:UP000002157};
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T.,
RA Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P.;
RT "Complete sequence of Pseudomonas putida GB-1.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC {ECO:0000256|ARBA:ARBA00008017}.
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DR EMBL; CP000926; ABZ01002.1; -; Genomic_DNA.
DR RefSeq; WP_012274620.1; NC_010322.1.
DR AlphaFoldDB; B0KN21; -.
DR KEGG; ppg:PputGB1_5117; -.
DR eggNOG; COG3264; Bacteria.
DR HOGENOM; CLU_007829_3_0_6; -.
DR Proteomes; UP000002157; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR Gene3D; 1.10.287.1260; -; 1.
DR Gene3D; 2.30.30.60; -; 1.
DR Gene3D; 3.30.70.100; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR049142; MS_channel_1st.
DR InterPro; IPR049278; MS_channel_C.
DR InterPro; IPR023408; MscS_beta-dom_sf.
DR InterPro; IPR006685; MscS_channel_2nd.
DR InterPro; IPR011066; MscS_channel_C_sf.
DR InterPro; IPR006686; MscS_channel_CS.
DR InterPro; IPR011014; MscS_channel_TM-2.
DR InterPro; IPR025692; MscS_IM_dom1.
DR InterPro; IPR024393; MscS_porin.
DR PANTHER; PTHR30347:SF8; MECHANOSENSITIVE CHANNEL MSCK; 1.
DR PANTHER; PTHR30347; POTASSIUM CHANNEL RELATED; 1.
DR Pfam; PF21088; MS_channel_1st; 1.
DR Pfam; PF00924; MS_channel_2nd; 1.
DR Pfam; PF21082; MS_channel_3rd; 1.
DR Pfam; PF12795; MscS_porin; 1.
DR Pfam; PF12794; MscS_TM; 1.
DR SUPFAM; SSF82689; Mechanosensitive channel protein MscS (YggB), C-terminal domain; 1.
DR SUPFAM; SSF82861; Mechanosensitive channel protein MscS (YggB), transmembrane region; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS01246; UPF0003; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..1102
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002749563"
FT TRANSMEM 491..507
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 536..559
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 571..591
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 612..631
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 643..664
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 676..699
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 711..731
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 779..803
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 829..850
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 871..892
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 898..925
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 37..270
FT /note="Mechanosensitive ion channel MscS porin"
FT /evidence="ECO:0000259|Pfam:PF12795"
FT DOMAIN 491..809
FT /note="Mechanosensitive ion channel inner membrane"
FT /evidence="ECO:0000259|Pfam:PF12794"
FT DOMAIN 870..911
FT /note="Mechanosensitive ion channel transmembrane helices
FT 2/3"
FT /evidence="ECO:0000259|Pfam:PF21088"
FT DOMAIN 913..978
FT /note="Mechanosensitive ion channel MscS"
FT /evidence="ECO:0000259|Pfam:PF00924"
FT DOMAIN 986..1069
FT /note="Mechanosensitive ion channel MscS C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21082"
FT COILED 221..251
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1102 AA; 122634 MW; 338574569F8B770A CRC64;
MSLRAYLRTA LLGLCLSLPF AAYAAEAPTT ASIQNSLDKI AERKLPEADQ KALQQVLEQT
LSLLASKEDN EKKLAALKQQ LTSAPKETSD NQKELARLKE SKALPVAQRY ATLTVPQLEQ
MLSERNTQQG ELQKALSEAN SLIINSQTRP ERAQAEISSS QTRTQQINNI LKTGKDGGKS
INADQRNQLN AELASLNALT LLRRQELAGN SLLQDLGNAR HDLLIERAAR LEQEIQDLQT
LINDKRLAQS QEAVTQQSIE AQKAGGSSLL ATESAVNLKL SDYLLKSTDR LNELTQQNLR
TKQQLDSLTQ ADQALDEQIN VLKGSLLLSK ILYKQKQTLP HLKVDRDLAD QIADTRLYQF
EVNQQREQMS SPVTYVDKLL AAQPQEDVTP QLRKALLEVA ITRSDLLERL NRELSALLNE
SITLQLNQKQ LLGTAQSLRT TLDEQMFWIP SNKPLDWDWL RYVPERLADQ VANLPWGSGL
KELADGLSQR PLLFLPLLLV IGALLWRRKY LYQRLGKVHQ DIGHFRRDSQ WHTPQAILIN
ILLAMPVSLG LALCSYALQI DARGQNANLG AALWQLAQAW LVFYTAYRIL APGGVAEIHF
RWHKPQVEFL RGWVRRLGTV VLALVGVVAV AEHQPSALAD DVLGIGVVLT CYALMAWLLS
RLLLSSPAHR DTSLFRRAVG VAFTALPIAL FVAVCFGYYY TALKLTDRLI YTLYLLLFWL
VIEAAFVRGL SVAARRLAYQ RALSKRAAAK EGLDGEVITE EPTLDIEQVN QQSLRLIRLA
LLGGFIAGLY WVWSDLISVF AYLNNFVLYE YTSGTGAAAS MVPISLGDLL GALVIVGITF
ALAGNLPGLL EVLVLSRLNL AQGSAYATTT LLSYTIVGVG IVSTLSTLGV SWDKLQWLVA
ALSVGLGFGM QEIFANFISG IMILFERPVR IGDTITIGNL SGTVSKIRIR ATTITDFDRK
DIIVPNKTFI TGQLINWSLT DTVTRVTLKL GIDYGSDLDL VRELLLKGAH ENPRVLKDPE
PIVYFLNFGE SSLDHELRMH VRDLGDRNPT LDEINRYINR EFKAHNIKIS VRQVEVFLMD
QKGGKQQLIP LEQPKPDGTA QA
//
