ID B0X288_CULQU Unreviewed; 543 AA.
AC B0X288;
DT 08-APR-2008, integrated into UniProtKB/TrEMBL.
DT 08-APR-2008, sequence version 1.
DT 27-MAR-2024, entry version 94.
DE RecName: Full=microtubule-severing ATPase {ECO:0000256|ARBA:ARBA00038871};
DE EC=5.6.1.1 {ECO:0000256|ARBA:ARBA00038871};
GN Name=6046584 {ECO:0000313|EnsemblMetazoa:CPIJ013667-PA};
GN ORFNames=CpipJ_CPIJ013667 {ECO:0000313|EMBL:EDS39111.1};
OS Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Culicini; Culex; Culex.
OX NCBI_TaxID=7176;
RN [1] {ECO:0000313|EMBL:EDS39111.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JHB {ECO:0000313|EMBL:EDS39111.1};
RG The Broad Institute Genome Sequencing Platform;
RA Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.,
RA Hannick L., Megy K., O'Leary S., Pearson M., Haas B.J., Mauceli E.,
RA Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA Fraser-Liggett C., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT "Annotation of Culex pipiens quinquefasciatus.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:CPIJ013667-PA}
RP IDENTIFICATION.
RC STRAIN=JHB {ECO:0000313|EnsemblMetazoa:CPIJ013667-PA};
RG EnsemblMetazoa;
RL Submitted (FEB-2021) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1)
CC alpha/beta tubulin heterodimers.; EC=5.6.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00036378};
CC -!- SIMILARITY: Belongs to the AAA ATPase family.
CC {ECO:0000256|RuleBase:RU003651}.
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DR EMBL; DS232283; EDS39111.1; -; Genomic_DNA.
DR RefSeq; XP_001863760.1; XM_001863725.1.
DR AlphaFoldDB; B0X288; -.
DR STRING; 7176.B0X288; -.
DR EnsemblMetazoa; CPIJ013667-RA; CPIJ013667-PA; CPIJ013667.
DR KEGG; cqu:CpipJ_CPIJ013667; -.
DR VEuPathDB; VectorBase:CPIJ013667; -.
DR VEuPathDB; VectorBase:CQUJHB018481; -.
DR eggNOG; KOG0740; Eukaryota.
DR HOGENOM; CLU_000688_21_2_1; -.
DR InParanoid; B0X288; -.
DR OMA; LCQEAIW; -.
DR OrthoDB; 276256at2759; -.
DR Proteomes; UP000002320; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IEA:UniProt.
DR CDD; cd19524; RecA-like_spastin; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015415; Spast_Vps4_C.
DR PANTHER; PTHR23074; AAA DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR23074:SF86; SPASTIN; 1.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF17862; AAA_lid_3; 1.
DR Pfam; PF09336; Vps4_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00674; AAA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU003651};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00023212};
KW Cytoskeleton {ECO:0000256|ARBA:ARBA00023212};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Microtubule {ECO:0000256|ARBA:ARBA00022701};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU003651};
KW Reference proteome {ECO:0000313|Proteomes:UP000002320}.
FT DOMAIN 300..437
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 35..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..61
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 79..100
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 209..242
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 543 AA; 58203 MW; 3B0F0A19C596A2A0 CRC64;
MESSECMLEA NKLEISEAHK LQRSNTFTLS VGQPTVLPTA QPARPATSYA PTTTTTTVKR
AQFKVPHPAK GALARPATAG RGQQQQQENR VMSYGDSNGG SGRNAPEPEA SGRKLTVGYK
RPGNLGVMNK SQTLPRSMGG SRTTPTGGSG AAGTGYGVGP ATAGGAPGCT AGGGMPKIVP
KPAATPPAIR RQFSIPSGSP IRKASNGYGH SGGKNTPPRS KTPLGTAPSN NQSGQQQQQQ
ITVKGVEPKL VQIIMDEIVE GGAKVEWQDI AGQEVAKQAL QEMVILPSVR PELFTGLRTP
AKGLLLFGPP GNGKTLLARA VATECSATFF SISAATLTSK YVGDGEKLVR ALFSVAREMQ
PAIIFIDEVD SLLSERSSGE HEATRRLKTE FLVQFDGLPA NSEADKIVVM AATNRPQELD
EAALRRFPKR VYVTLPDLDT RELLLRRLLE KQNSPLDDAD LKRLAMLTEG YSGSDLTALA
KDAALEPIRE LNVEQVKHMD PTKLRSIRES DFHNSLKRIR RSVAPHSLAA YEKWLQDFGD
VTL
//
