ID   B0X3L1_CULQU            Unreviewed;       256 AA.
AC   B0X3L1;
DT   08-APR-2008, integrated into UniProtKB/TrEMBL.
DT   08-APR-2008, sequence version 1.
DT   27-MAR-2024, entry version 73.
DE   RecName: Full=tRNA-uridine aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00012386};
DE            EC=2.5.1.25 {ECO:0000256|ARBA:ARBA00012386};
GN   Name=6047149 {ECO:0000313|EnsemblMetazoa:CPIJ013359-PA};
GN   ORFNames=CpipJ_CPIJ013359 {ECO:0000313|EMBL:EDS39903.1};
OS   Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Culex; Culex.
OX   NCBI_TaxID=7176;
RN   [1] {ECO:0000313|EMBL:EDS39903.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JHB {ECO:0000313|EMBL:EDS39903.1};
RG   The Broad Institute Genome Sequencing Platform;
RA   Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.,
RA   Hannick L., Megy K., O'Leary S., Pearson M., Haas B.J., Mauceli E.,
RA   Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA   Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA   Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA   Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA   Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA   Fraser-Liggett C., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT   "Annotation of Culex pipiens quinquefasciatus.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblMetazoa:CPIJ013359-PA}
RP   IDENTIFICATION.
RC   STRAIN=JHB {ECO:0000313|EnsemblMetazoa:CPIJ013359-PA};
RG   EnsemblMetazoa;
RL   Submitted (MAY-2020) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC         amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC         thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC         COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC         Evidence={ECO:0000256|ARBA:ARBA00024168};
CC   -!- SIMILARITY: Belongs to the TDD superfamily. DTWD2 family.
CC       {ECO:0000256|ARBA:ARBA00034489}.
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DR   EMBL; DS232317; EDS39903.1; -; Genomic_DNA.
DR   RefSeq; XP_001864233.1; XM_001864198.1.
DR   AlphaFoldDB; B0X3L1; -.
DR   STRING; 7176.B0X3L1; -.
DR   EnsemblMetazoa; CPIJ013359-RA; CPIJ013359-PA; CPIJ013359.
DR   KEGG; cqu:CpipJ_CPIJ013359; -.
DR   VEuPathDB; VectorBase:CPIJ013359; -.
DR   VEuPathDB; VectorBase:CQUJHB017980; -.
DR   eggNOG; KOG4382; Eukaryota.
DR   HOGENOM; CLU_066458_3_0_1; -.
DR   InParanoid; B0X3L1; -.
DR   OMA; GTWRKAF; -.
DR   Proteomes; UP000002320; Unassembled WGS sequence.
DR   GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR005636; DTW.
DR   InterPro; IPR039262; DTWD2/TAPT.
DR   PANTHER; PTHR21392:SF0; TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2; 1.
DR   PANTHER; PTHR21392; UNCHARACTERIZED; 1.
DR   Pfam; PF03942; DTW; 1.
DR   SMART; SM01144; DTW; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000002320};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT   DOMAIN          22..215
FT                   /note="DTW"
FT                   /evidence="ECO:0000259|SMART:SM01144"
SQ   SEQUENCE   256 AA;  28700 MW;  D53E9358AE28EDEA CRC64;
     MTSLEEVTWA DLANIPADPP TMRELCKKCE RPVQVCWCSA LPPKPLEPRG RIIILQHPAE
     EKRSLRTAPM LSVGLAPGKC LIYKGKRFPK LDSDLESILA DEKSLLLYPS ASSVPLEQVA
     ASDDGPFNLI LIDGTWPQAK AIYHCSTALH TMRQVKLVST GNSNYVIRTQ PSDGCLSTLE
     TAIEALSVLE RDNSYREQLL RPLHALCQFQ LDNGAVTHQS KEFLIKHNQY PKLIGKRLNK
     LLRNADNLKE EKEVKH
//