ID   B1INZ1_CLOBK            Unreviewed;       972 AA.
AC   B1INZ1;
DT   29-APR-2008, integrated into UniProtKB/TrEMBL.
DT   29-APR-2008, sequence version 1.
DT   27-MAR-2024, entry version 64.
DE   RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE            EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN   OrderedLocusNames=CLD_A0164 {ECO:0000313|EMBL:ACA46996.1};
OS   Clostridium botulinum (strain Okra / Type B1).
OG   Plasmid pCLD {ECO:0000313|EMBL:ACA46996.1,
OG   ECO:0000313|Proteomes:UP000008541}.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=498213 {ECO:0000313|EMBL:ACA46996.1, ECO:0000313|Proteomes:UP000008541};
RN   [1] {ECO:0000313|EMBL:ACA46996.1, ECO:0000313|Proteomes:UP000008541}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Okra / Type B1 {ECO:0000313|Proteomes:UP000008541};
RC   PLASMID=pCLD {ECO:0000313|EMBL:ACA46996.1,
RC   ECO:0000313|Proteomes:UP000008541};
RX   PubMed=18060065; DOI=10.1371/journal.pone.0001271;
RA   Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C.,
RA   Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.;
RT   "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4
RT   and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within
RT   plasmids.";
RL   PLoS ONE 2:E1271-E1271(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR   EMBL; CP000940; ACA46996.1; -; Genomic_DNA.
DR   RefSeq; WP_012291522.1; NC_010379.1.
DR   AlphaFoldDB; B1INZ1; -.
DR   REBASE; 17431; CboB1ORF164P.
DR   KEGG; cbb:CLD_A0164; -.
DR   HOGENOM; CLU_007039_0_0_9; -.
DR   Proteomes; UP000008541; Plasmid pCLD.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR003356; DNA_methylase_A-5.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR   PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR   Pfam; PF02384; N6_Mtase; 1.
DR   PRINTS; PR00507; N12N6MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   4: Predicted;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW   Plasmid {ECO:0000313|EMBL:ACA46996.1};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          288..555
FT                   /note="DNA methylase adenine-specific"
FT                   /evidence="ECO:0000259|Pfam:PF02384"
SQ   SEQUENCE   972 AA;  113420 MW;  5A33AC198B6755CD CRC64;
     MVNRISNTTQ MIIKKLGYHN SKNLYYFANI NMCKDLSFHD KKVLNEIKPC AFFVVNGKPK
     VLFFDYISEN DSKQKLYKKI WNAQIPIIIF NDYDSIKVFN GNSMDLSELD NIRLQFVLEE
     EHKKCSEASP FSYWNITNEQ FLREYQIAFS KDTLNQVMIK NIKCITEKLK KQYRVKFATK
     LILRLIFIRF LIDRGIDIGY DGFNGDIKES QEALLKLANN KRKLYSFFSY LKNKFNGNLF
     ELEDEVYDEA LNDEVFELLK CFLSGNQEME SGQLSFLPLY DFNIIPIELI SNIYEVLLGE
     KAQDDDKAFY TPEYLADYIV KESLGTFLTK DSQCKVLDPS CGSGIFLVES LQLIISKNVD
     DNGYIKDNDK LCQLIESNIY GVDSNPEAID VTIFSLYLTL FDYKDPKSLD DFRLPNLKNK
     NLWVSDFFDD EKLIALKKIK FQFILGNPPW GSVKEGLHSQ YCDENKIPQY RQEISRSFIA
     KVKEYSNEDT ICCLIVPSKL FYNQKKPAIE FRKLLLLKCK ILQIVELSSV RSLIFKKADA
     PAAILMFKNS TENCLSHKML HLSLKPNMFF KIYHVIAIEK TDIKNIQQDI LYRYDWAWKT
     CVYGNSWDID IITMLKRKFP KIEDVINKNK LKTGAGITDT NGKYDAKDYI GKNMIESTAI
     DTLYFNSSNS SIFNKRKIYR LGKKEIFSPP YCLLRKGPNC KNYRLRAAYT EEDVIFKEAI
     SAIKGDKSQK DLLLNITGMF NSSLYAYLNL MLGSSMGIER EQVFMKEVYE YPFAYDEGIS
     SKVEAIQKKN SNLKNGFYQD ISQDISQLDQ MILDKFGLKE DPFIDYAINV QIPLLNSKKL
     TFREVTAEEL KDYSKVFVDY WKKLMAEQNK FIKISLYPKV VGKFAIFELQ ICDQEEKEIT
     IIDTIDSNKE ILSRFLINKI NEQFYKLKDV LYFENNSFFI IKSNEYKNWH AAMSHIDQSM
     VLETIISDDS EE
//