ID B1YL62_EXIS2 Unreviewed; 970 AA.
AC B1YL62;
DT 20-MAY-2008, integrated into UniProtKB/TrEMBL.
DT 20-MAY-2008, sequence version 1.
DT 27-MAR-2024, entry version 104.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN OrderedLocusNames=Exig_0813 {ECO:0000313|EMBL:ACB60294.1};
OS Exiguobacterium sibiricum (strain DSM 17290 / CIP 109462 / JCM 13490 /
OS 255-15).
OC Bacteria; Bacillota; Bacilli; Bacillales;
OC Bacillales Family XII. Incertae Sedis; Exiguobacterium.
OX NCBI_TaxID=262543 {ECO:0000313|EMBL:ACB60294.1, ECO:0000313|Proteomes:UP000001681};
RN [1] {ECO:0000313|EMBL:ACB60294.1, ECO:0000313|Proteomes:UP000001681}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17290 / CIP 109462 / JCM 13490 / 255-15
RC {ECO:0000313|Proteomes:UP000001681};
RX PubMed=16489412; DOI=10.1007/s00792-005-0497-5;
RA Rodrigues D.F., Goris J., Vishnivetskaya T., Gilichinsky D.,
RA Thomashow M.F., Tiedje J.M.;
RT "Characterization of Exiguobacterium isolates from the Siberian permafrost.
RT Description of Exiguobacterium sibiricum sp. nov.";
RL Extremophiles 10:285-294(2006).
RN [2] {ECO:0000313|EMBL:ACB60294.1, ECO:0000313|Proteomes:UP000001681}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17290 / CIP 109462 / JCM 13490 / 255-15
RC {ECO:0000313|Proteomes:UP000001681};
RX PubMed=19019206; DOI=10.1186/1471-2164-9-547;
RA Rodrigues D.F., Ivanova N., He Z., Huebner M., Zhou J., Tiedje J.M.;
RT "Architecture of thermal adaptation in an Exiguobacterium sibiricum strain
RT isolated from 3 million year old permafrost: a genome and transcriptome
RT approach.";
RL BMC Genomics 9:547-547(2008).
RN [3] {ECO:0000313|Proteomes:UP000001681}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17290 / CIP 109462 / JCM 13490 / 255-15
RC {ECO:0000313|Proteomes:UP000001681};
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C.,
RA Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F.,
RA Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T.,
RA Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome of Exiguobacterium sibiricum 255-15.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001022; ACB60294.1; -; Genomic_DNA.
DR RefSeq; WP_012369718.1; NC_010556.1.
DR AlphaFoldDB; B1YL62; -.
DR STRING; 262543.Exig_0813; -.
DR CAZy; CBM41; Carbohydrate-Binding Module Family 41.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR KEGG; esi:Exig_0813; -.
DR eggNOG; COG1523; Bacteria.
DR HOGENOM; CLU_004744_1_0_9; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000001681; Chromosome.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd10315; CBM41_pullulanase; 2.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 2.60.40.1110; -; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR005323; CBM41_pullulanase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR049117; pulA_all-beta.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF03714; PUD; 2.
DR Pfam; PF21653; pulA_all-beta; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 2.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000001681}.
FT DOMAIN 500..874
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 970 AA; 108455 MW; 3C02281B92771D91 CRC64;
MNRWKSVCAV VLTFALIFSL FPVNGLALEK GKSTLVIIHY KEDKTAPKDW NLWLWANGPD
YFPNKAHAFN GQDAYGKIAA YEFPVDQPEK IGFIVRDDSW NKDAGSENDR FITKDMIKDG
VAEVWIESGS KDVSPVNPTG GSDVESVNTN IHYYRYDNDY DKYGVWAWPN AQDGKRFEFD
GQDAFGAVAN VTLTNTTKER LVGIIPHIEG WSEKDGADRF YYAGAKDIWL IEGDQTVYYS
QPDIDRTPKF TSFLADDFKT MTLKTNSPIA AEDVKTLTFK GLVNPVVTQV DAKTLHVTVA
SDIDLANVVT VSHPVFGEGI LEAGKVLRSD AFDKKYAYDG KLGTAYQKGK TTFRVWAPTA
QSVELELYKL PKKQAATTKD IVREVKMVRG DRGVFSITVN GDLDGTGYTY EVKHAKETTR
AVDPYTTAVA VNGDQGVVVD LRETNPKRWT KTKMPLKQAT DAIIYETHVR DLSMFDVTGD
TYDSGIEQKG KYLGVVKPGT RLMKDGKKTK TKTGLDHLKE LGITHVQFIP VYDFNTASVD
ETNPLKTYNW GYDPKNYNAP EGSYSTNPYD PKVRIKEMKQ MIQGLHDNGL RAVMDVVYNH
MFDAKASNLD KIVPGYYYRY TEGGDLANGT GVGNDTASER KMMHKLIVDS VGYWAKEYRW
DGFRFDLMGI HDVKTMNAVK KTTTKIDPSI LVFGEGWSLG TPLPEADKAN QTNAKQMPGI
AHFNDNLRDA LKGSVFEDTD AGFINGKTGL ETRIKRGIVG EIAYNKDITG FAAEPNQAIT
YVEAHDNHTL WDKLNLTNPN DTDATKTKMH RLASSILLTS QGTTFIHAGQ DFMRTKGGDH
NSYKSPDAVN QLDWKRKMDR QADVDYMKGL IQVRKANPVL HLGTAKDIRR YLTFKDVPEQ
VVAYQLSDDA PQQKTDLYVV HNAKRQAVKM TLPKGTWSVI VDGKTAGMKT LKKVSGSVTV
DPLSSYVLKK
//
