ID MIAB_METPB Reviewed; 446 AA.
AC B1ZJR5;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 01-MAY-2013, entry version 37.
DE RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB;
DE EC=2.-.-.-;
DE AltName: Full=tRNA-i(6)A37 methylthiotransferase;
GN Name=miaB; OrderedLocusNames=Mpop_3399;
OS Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Methylobacteriaceae; Methylobacterium.
OX NCBI_TaxID=441620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-705 / NCIMB 13946 / BJ001;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E.,
RA Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T.,
RA Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P.;
RT "Complete sequence of chromosome of Methylobacterium populi BJ001.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the methylthiolation of N6-
CC (dimethylallyl)adenosine (i(6)A), leading to the formation of 2-
CC methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37
CC in tRNAs that read codons beginning with uridine (By similarity).
CC -!- COFACTOR: Binds 2 4Fe-4S clusters. One cluster is coordinated with
CC 3 cysteines and an exchangeable S-adenosyl-L-methionine (By
CC similarity).
CC -!- SUBUNIT: Monomer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the methylthiotransferase family. MiaB
CC subfamily.
CC -!- SIMILARITY: Contains 1 MTTase N-terminal domain.
CC -!- SIMILARITY: Contains 1 TRAM domain.
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DR EMBL; CP001029; ACB81550.1; -; Genomic_DNA.
DR RefSeq; YP_001926085.1; NC_010725.1.
DR ProteinModelPortal; B1ZJR5; -.
DR STRING; 441620.Mpop_3399; -.
DR EnsemblBacteria; ACB81550; ACB81550; Mpop_3399.
DR GeneID; 6311435; -.
DR KEGG; mpo:Mpop_3399; -.
DR PATRIC; 22564104; VBIMetPop124700_3457.
DR eggNOG; COG0621; -.
DR HOGENOM; HOG000224767; -.
DR KO; K06168; -.
DR OMA; GIDRIRY; -.
DR ProtClustDB; PRK14325; -.
DR BioCyc; MPOP441620:GHMI-3482-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:HAMAP.
DR GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR GO; GO:0016740; F:transferase activity; IEA:HAMAP.
DR GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR Gene3D; 3.80.30.20; -; 1.
DR HAMAP; MF_01864; tRNA_metthiotr_MiaB; 1; -.
DR InterPro; IPR006638; Elp3/MiaB/NifB.
DR InterPro; IPR023970; MeThioTfrase/rSAM.
DR InterPro; IPR005839; Methylthiotransferase.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR013848; Methylthiotransferase_N.
DR InterPro; IPR006463; MiaB_methiolase.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR002792; TRAM_dom.
DR PANTHER; PTHR11918; PTHR11918; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF00919; UPF0004; 1.
DR SMART; SM00729; Elp3; 1.
DR TIGRFAMs; TIGR01574; miaB-methiolase; 1.
DR TIGRFAMs; TIGR00089; TIGR00089; 1.
DR PROSITE; PS51449; MTTASE_N; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS50926; TRAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;
KW Metal-binding; S-adenosyl-L-methionine; Transferase; tRNA processing.
FT CHAIN 1 446 (Dimethylallyl)adenosine tRNA
FT methylthiotransferase MiaB.
FT /FTId=PRO_0000374378.
FT DOMAIN 2 122 MTTase N-terminal.
FT DOMAIN 378 440 TRAM.
FT METAL 11 11 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 47 47 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 85 85 Iron-sulfur (4Fe-4S) (By similarity).
FT METAL 157 157 Iron-sulfur (4Fe-4S-S-AdoMet) (By
FT similarity).
FT METAL 161 161 Iron-sulfur (4Fe-4S-S-AdoMet) (By
FT similarity).
FT METAL 164 164 Iron-sulfur (4Fe-4S-S-AdoMet) (By
FT similarity).
SQ SEQUENCE 446 AA; 48091 MW; 8E946FAD0CBBA5AB CRC64;
MKKAYVKSYG CQMNAYDAGR MADVLAAEGY SATESVEEAD VVVLNTCHIR EKAAEKVYSE
LGRLRVLKGE RAESGQDTRI VVAGCVAQAE GREILSRAPA VDVVVGPQSY HRLPDLLRQS
RETRVVDTEF PVEDKFDHLP ARRNRGVTGF LTVQEGCDKF CAFCVVPYTR GAEVSRSVAA
VVEEARRLVE GGVREITLIG QNVNAYHGDG PDGAPATLGH LMDALSAVPG LLRLRYTTSH
PNDFADDLIA AHAGNPLVMP YLHLPVQSGS DRILHAMNRR HTGDAYRRLI ERIRTARPDI
ALSSDFIVGF PGESDADFAE TMRLVAEIGF AAAFSFKYSP RAGTPAAERE DAVPEAVKTE
RLAALQQLLD QQRHAFNAAA VGTVAEILVE KTGRHPGQVA GKTPHLQAVQ FDAPASTIGT
LVPVRITRAG SNSLFGEVLE GAAAAA
//
