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Database: UniProt
Entry: B2A334
LinkDB: B2A334
Original site: B2A334 
ID   TRMFO_NATTJ             Reviewed;         433 AA.
AC   B2A334;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   29-OCT-2014, entry version 49.
DE   RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO {ECO:0000255|HAMAP-Rule:MF_01037};
DE            EC=2.1.1.74 {ECO:0000255|HAMAP-Rule:MF_01037};
DE   AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01037};
DE   AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01037};
GN   Name=trmFO {ECO:0000255|HAMAP-Rule:MF_01037};
GN   OrderedLocusNames=Nther_1382;
OS   Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 /
OS   JW/NM-WN-LF).
OC   Bacteria; Firmicutes; Clostridia; Natranaerobiales; Natranaerobiaceae;
OC   Natranaerobius.
OX   NCBI_TaxID=457570;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T.,
RA   Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.;
RT   "Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-
RT   WN-LF.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the folate-dependent formation of 5-methyl-
CC       uridine at position 54 (M-5-U54) in all tRNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01037}.
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + uracil(54) in
CC       tRNA + FADH(2) = tetrahydrofolate + 5-methyluracil(54) in tRNA +
CC       FAD. {ECO:0000255|HAMAP-Rule:MF_01037}.
CC   -!- COFACTOR: FAD. {ECO:0000255|HAMAP-Rule:MF_01037}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01037}.
CC   -!- SIMILARITY: Belongs to the MnmG family. TrmFO subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01037}.
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DR   EMBL; CP001034; ACB84965.1; -; Genomic_DNA.
DR   RefSeq; WP_012447840.1; NC_010718.1.
DR   RefSeq; YP_001917553.1; NC_010718.1.
DR   ProteinModelPortal; B2A334; -.
DR   STRING; 457570.Nther_1382; -.
DR   EnsemblBacteria; ACB84965; ACB84965; Nther_1382.
DR   GeneID; 6316635; -.
DR   KEGG; nth:Nther_1382; -.
DR   PATRIC; 22671430; VBINatThe92436_1417.
DR   eggNOG; COG1206; -.
DR   HOGENOM; HOG000252054; -.
DR   KO; K04094; -.
DR   OMA; EIYFEGC; -.
DR   OrthoDB; EOG6J74VT; -.
DR   BioCyc; NTHE457570:GHRL-1418-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030698; F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0047151; F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity; IEA:UniProtKB-EC.
DR   HAMAP; MF_01037; TrmFO; 1.
DR   InterPro; IPR004417; Folate-dep_Ribothymidyl_synth.
DR   InterPro; IPR002218; GIDA-rel.
DR   Pfam; PF01134; GIDA; 1.
DR   TIGRFAMs; TIGR00137; gid_trmFO; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Methyltransferase;
KW   Reference proteome; Transferase; tRNA processing.
FT   CHAIN         1    433       Methylenetetrahydrofolate--tRNA-(uracil-
FT                                5-)-methyltransferase TrmFO.
FT                                /FTId=PRO_0000346366.
FT   NP_BIND       7     12       FAD. {ECO:0000255|HAMAP-Rule:MF_01037}.
SQ   SEQUENCE   433 AA;  48280 MW;  5CBC6436875B61AD CRC64;
     MRLTVIGGGL AGSEAAYQAA QQGVTVDLYE MRPVKTTAAH KTGELAELVC SNSLRAKSLE
     NAAGLLKEEL RKFDSLIMKV ADQVQVPAGG ALAVDREAFS QKVTEYIENH PNINLITEEV
     TEINESQPTV IATGPLTSTS LTERIQKLTG TDFLYFYDAA APIVTYESIN KNIAYWASRY
     GKGTPDYLNC PMTKEEYEDF YQELINAEKV PLKSFEKEVV FEGCMPVEQM ANRGKDTLVF
     GPLKPVGLEN PNTGEMPYAV VQLRKDNREG TLYNLVGFQT RLKWPEQRRV FRKIPGLEEA
     EFVRYGVMHR NTFINSPKVL TANYQLRERP GLFFAGQVTG VEGYVESTAS GLVAGLNAAR
     KLQGNETLTF PRETALGALA SYIVEASPDN FQPMNINFGL LPSLEKKVPK KIKKQKQSER
     ALKILEEFIE RKL
//
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