ID GAL1_LACRJ Reviewed; 392 AA.
AC B2G9P6;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 01-MAY-2013, entry version 37.
DE RecName: Full=Galactokinase;
DE EC=2.7.1.6;
DE AltName: Full=Galactose kinase;
GN Name=galK; OrderedLocusNames=LAR_1662;
OS Lactobacillus reuteri (strain JCM 1112).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=557433;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 1112;
RX PubMed=18487258; DOI=10.1093/dnares/dsn009;
RA Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T.,
RA Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H.,
RA Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H.,
RA Kikuchi J., Masaoka T., Hattori M.;
RT "Comparative genome analysis of Lactobacillus reuteri and
RT Lactobacillus fermentum reveal a genomic island for reuterin and
RT cobalamin production.";
RL DNA Res. 15:151-161(2008).
CC -!- FUNCTION: Catalyzes the transfer of the gamma-phosphate of ATP to
CC D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P) (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: ATP + D-galactose = ADP + alpha-D-galactose 1-
CC phosphate.
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the GHMP kinase family. GalK subfamily.
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DR EMBL; AP007281; BAG26178.1; -; Genomic_DNA.
DR RefSeq; YP_001842658.1; NC_010609.1.
DR ProteinModelPortal; B2G9P6; -.
DR SMR; B2G9P6; 5-392.
DR STRING; 557433.LAR_1662; -.
DR EnsemblBacteria; BAG26178; BAG26178; LAR_1662.
DR GeneID; 6230905; -.
DR KEGG; lrf:LAR_1662; -.
DR PATRIC; 22260999; VBILacReu111271_1833.
DR eggNOG; COG0153; -.
DR HOGENOM; HOG000241101; -.
DR KO; K00849; -.
DR OMA; MPCAINY; -.
DR ProtClustDB; PRK05322; -.
DR BioCyc; LREU557433:GHNR-1663-MONOMER; -.
DR UniPathway; UPA00214; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0004335; F:galactokinase activity; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0006012; P:galactose metabolic process; IEA:HAMAP.
DR Gene3D; 3.30.230.10; -; 1.
DR HAMAP; MF_00246; Galactokinase; 1; -.
DR InterPro; IPR000705; Galactokinase.
DR InterPro; IPR022963; Galactokinase_bac.
DR InterPro; IPR019741; Galactokinase_CS.
DR InterPro; IPR019539; GalKase_gal-bd.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR InterPro; IPR006206; Mevalonate/galactokinase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR Pfam; PF10509; GalKase_gal_bdg; 1.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF000530; Galactokinase; 1.
DR PRINTS; PR00473; GALCTOKINASE.
DR PRINTS; PR00959; MEVGALKINASE.
DR SUPFAM; SSF54211; Ribosomal_S5_D2-typ_fold; 1.
DR TIGRFAMs; TIGR00131; gal_kin; 1.
DR PROSITE; PS00106; GALACTOKINASE; 1.
DR PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE 3: Inferred from homology;
KW ATP-binding; Carbohydrate metabolism; Complete proteome; Cytoplasm;
KW Galactose metabolism; Kinase; Magnesium; Metal-binding;
KW Nucleotide-binding; Transferase.
FT CHAIN 1 392 Galactokinase.
FT /FTId=PRO_1000100833.
FT NP_BIND 129 135 ATP (By similarity).
FT REGION 33 36 Substrate binding (By similarity).
FT ACT_SITE 179 179 Proton acceptor (By similarity).
FT METAL 135 135 Magnesium (By similarity).
FT METAL 167 167 Magnesium (By similarity).
FT BINDING 67 67 ATP (By similarity).
FT BINDING 229 229 Substrate (By similarity).
FT SITE 27 27 Transition state stabilizer (By
FT similarity).
SQ SEQUENCE 392 AA; 44056 MW; 2877C04471F8200A CRC64;
MDKQQFLAEY QDVFKEPGKD VFFSPGRINV IGEHTDYNGG HVFPCAISIG TYGVYGPRED
TTVAIYSANS AKEEDSKIIT FDINDTEPQN AKDEKWVNYF KGMLVYLKQR GFKIDHGFNL
YIHGFLPYGS GLSSSASIEM LMGNILKDEF NLDIDEIELV KLGQKTENDF VGLNSGIMDQ
FAVGMGKENN AIYLDCNTLE YKYLPLELGD YEIIIMSTNK NHSLAGSKYN ERVQECEEAV
KRLNKKLDIN KLGELDSDTF DQYTYLIDDD TLIRRARHAV SENERTKKAI DAMEKGDLEE
LGRLINASHV SLKYDYEVTG KELDTLAENA WNQPGCLGAR MVGGGFAGSA IAIVKKSEAE
NFKKNVGKIY RDKIGYDASF YDAEVVDGPH KL
//
