ID B2J5N4_NOSP7 Unreviewed; 1037 AA.
AC B2J5N4;
DT 10-JUN-2008, integrated into UniProtKB/TrEMBL.
DT 10-JUN-2008, sequence version 1.
DT 27-MAR-2024, entry version 92.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN OrderedLocusNames=Npun_F5459 {ECO:0000313|EMBL:ACC83766.1};
OS Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=63737 {ECO:0000313|EMBL:ACC83766.1, ECO:0000313|Proteomes:UP000001191};
RN [1] {ECO:0000313|Proteomes:UP000001191}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29133 / PCC 73102 {ECO:0000313|Proteomes:UP000001191};
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ACC83766.1, ECO:0000313|Proteomes:UP000001191}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29133 / PCC 73102 {ECO:0000313|Proteomes:UP000001191};
RX PubMed=23463784; DOI=10.1104/pp.112.213116;
RA Ekman M., Picossi S., Campbell E.L., Meeks J.C., Flores E.;
RT "A Nostoc punctiforme Sugar Transporter Necessary to Establish a
RT Cyanobacterium-Plant Symbiosis.";
RL Plant Physiol. 161:1984-1992(2013).
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR EMBL; CP001037; ACC83766.1; -; Genomic_DNA.
DR RefSeq; WP_012411712.1; NC_010628.1.
DR AlphaFoldDB; B2J5N4; -.
DR STRING; 63737.Npun_F5459; -.
DR EnsemblBacteria; ACC83766; ACC83766; Npun_F5459.
DR KEGG; npu:Npun_F5459; -.
DR eggNOG; COG2352; Bacteria.
DR HOGENOM; CLU_006557_2_0_3; -.
DR OrthoDB; 9768133at2; -.
DR PhylomeDB; B2J5N4; -.
DR Proteomes; UP000001191; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:ACC83766.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001191}.
FT REGION 109..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 640..671
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..137
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 653..667
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 198
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 683
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 1037 AA; 118418 MW; 199A24903B067C9B CRC64;
MGSLLYSSSQ TADIYPVSEL FLRHRLQVVE ELWESVLRQE CGQNMVDLLR QLRDLCSPEG
QATNDQASSA VKLIEQLNIN EAIRAARAFA LYFQLINIIE QEYEQRQQLS RYEAETEPTD
SERASNVNYS SNQREDDAPV SKGIAAELLA KNYLEKAQVK PKGTFAALFP YLFKQNVPPQ
QIQRLIAHLD VRLVFTAHPT EIVRHTIRDK QRQVVQLLQK LDTLENHAGG TGGGYAWEAA
DVREQLLEEI RLWWRTDELH QFKPTVLDEV DYALHYFQEV LFDGIPQLYK RFKHTLSNTF
PWLEPPSKNF CSFGSWVGSD RDGNPSVTPE VTWQTACYQR KMVLGRYIQS VKNLIELLSV
SMHWSDVLPD LLESLELDQS QLSEVYDALA LRYRQEPYRL KLAYVLKRLE NTRDRNLALY
NRETPKNQDS PLYRSGDDFL AELRMIQRNL TETGLSCRDL ENLICQVEIF GFNLTQLDIR
QESSRHADAL NEILQYLQIL PQPYNELSEE QKVAWLTGEL QTRRPLIPAE LPFSEKTNDV
IETFRVVRSL QQEFGLNICQ TYIISMCRDV SDVLEVLLLA KEARLFDPAI AVGTIQVVPL
FETVEDLQRS RSVMRKLFEL PLYRALLAGG YEQTKTESVV VDENSSSLAS PAPPASPASP
APSPSPLTPN LQEVMLGYSD SNKDSGFLSS NWEIHKAQKS LQQIAEGYDI NLRIFHGRGG
SVGRGGGPAY EAILAQPGHS INGRIKITEQ GEVLASKYSL LDLALYHMET ITTAVIQASL
LRTGFDDIEP WNEIMEELAA RSRQHYRALI YEQPDFVDFF HQVTPIEEIS QLQISSRPAR
RPSGKKDLSS LRAIPWVFSW TQTRFLLPSW YGVGTALQEF LNAEPEEHLK LMRYFYVKWP
FFKMVISKAE MTLAKVDMQM AHHYVQELSQ SEDQLRFAKV FDQIASEFYL TRDLVLKITD
HNQLLDGDPV LQRSVQLRNG TIVPLGFIQV SLLKRLRQSM NTNATSGVIH SRYSKGELLR
GALLTINGIA AGMRNTG
//
