ID B2WJH2_PYRTR Unreviewed; 2915 AA.
AC B2WJH2;
DT 01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT 01-JUL-2008, sequence version 1.
DT 24-JAN-2024, entry version 72.
DE SubName: Full=Pre-mRNA processing splicing factor 8 {ECO:0000313|EMBL:EDU43369.1};
GN ORFNames=PTRG_10318 {ECO:0000313|EMBL:EDU43369.1};
OS Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus)
OS (Drechslera tritici-repentis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae;
OC Pyrenophora.
OX NCBI_TaxID=426418 {ECO:0000313|EMBL:EDU43369.1, ECO:0000313|Proteomes:UP000001471};
RN [1] {ECO:0000313|Proteomes:UP000001471}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Pt-1C-BFP {ECO:0000313|Proteomes:UP000001471};
RX PubMed=23316438; DOI=10.1534/g3.112.004044;
RA Manning V.A., Pandelova I., Dhillon B., Wilhelm L.J., Goodwin S.B.,
RA Berlin A.M., Figueroa M., Freitag M., Hane J.K., Henrissat B., Holman W.H.,
RA Kodira C.D., Martin J., Oliver R.P., Robbertse B., Schackwitz W.,
RA Schwartz D.C., Spatafora J.W., Turgeon B.G., Yandava C., Young S., Zhou S.,
RA Zeng Q., Grigoriev I.V., Ma L.-J., Ciuffetti L.M.;
RT "Comparative genomics of a plant-pathogenic fungus, Pyrenophora tritici-
RT repentis, reveals transduplication and the impact of repeat elements on
RT pathogenicity and population divergence.";
RL G3 (Bethesda) 3:41-63(2013).
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DR EMBL; DS231627; EDU43369.1; -; Genomic_DNA.
DR RefSeq; XP_001940650.1; XM_001940615.1.
DR STRING; 426418.B2WJH2; -.
DR EnsemblFungi; EDU43369; EDU43369; PTRG_10318.
DR GeneID; 6348619; -.
DR eggNOG; KOG1795; Eukaryota.
DR HOGENOM; CLU_000380_3_0_1; -.
DR InParanoid; B2WJH2; -.
DR OMA; ANKWNTS; -.
DR OrthoDB; 246127at2759; -.
DR Proteomes; UP000001471; Unassembled WGS sequence.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IEA:EnsemblFungi.
DR GO; GO:0000974; C:Prp19 complex; IEA:EnsemblFungi.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IEA:EnsemblFungi.
DR GO; GO:0005682; C:U5 snRNP; IEA:EnsemblFungi.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IEA:EnsemblFungi.
DR GO; GO:0000386; F:second spliceosomal transesterification activity; IEA:EnsemblFungi.
DR GO; GO:0030619; F:U1 snRNA binding; IEA:EnsemblFungi.
DR GO; GO:0030620; F:U2 snRNA binding; IEA:EnsemblFungi.
DR GO; GO:0030623; F:U5 snRNA binding; IEA:EnsemblFungi.
DR GO; GO:0017070; F:U6 snRNA binding; IEA:EnsemblFungi.
DR GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IEA:EnsemblFungi.
DR GO; GO:0000389; P:mRNA 3'-splice site recognition; IEA:EnsemblFungi.
DR GO; GO:0000395; P:mRNA 5'-splice site recognition; IEA:EnsemblFungi.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IEA:EnsemblFungi.
DR CDD; cd08056; MPN_PRP8; 1.
DR CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR Gene3D; 1.20.80.40; -; 1.
DR Gene3D; 3.30.420.230; -; 1.
DR Gene3D; 3.90.1570.40; -; 1.
DR Gene3D; 3.40.140.10; Cytidine Deaminase, domain 2; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.30.43.40; Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain; 1.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR000555; JAMM/MPN+_dom.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR012591; PRO8NT.
DR InterPro; IPR012592; PROCN.
DR InterPro; IPR012984; PROCT.
DR InterPro; IPR027652; PRP8.
DR InterPro; IPR021983; PRP8_domainIV.
DR InterPro; IPR043173; Prp8_domainIV_fingers.
DR InterPro; IPR043172; Prp8_domainIV_palm.
DR InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR PANTHER; PTHR11140; PRE-MRNA SPLICING FACTOR PRP8; 1.
DR PANTHER; PTHR11140:SF0; PRE-MRNA-PROCESSING-SPLICING FACTOR 8; 1.
DR Pfam; PF08082; PRO8NT; 1.
DR Pfam; PF08083; PROCN; 1.
DR Pfam; PF08084; PROCT; 1.
DR Pfam; PF12134; PRP8_domainIV; 1.
DR Pfam; PF10598; RRM_4; 1.
DR Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR Pfam; PF10596; U6-snRNA_bdg; 2.
DR SMART; SM00232; JAB_MPN; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 2.
DR PROSITE; PS50249; MPN; 1.
PE 4: Predicted;
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000001471};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728}.
FT DOMAIN 2683..2814
FT /note="MPN"
FT /evidence="ECO:0000259|PROSITE:PS50249"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..53
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2915 AA; 336497 MW; 32C54FFAE83DE747 CRC64;
MAFPPPPPQQ PPPPPPPGPP GSGYAPPPPP PPPGPGPPPA PGTVGPPGMR PPIDPQIAKF
AQKKRDWVRQ QRQRFGEKRK AGFVETQKAD LPPEHLRKVF RDIGDVSQKK FTTDKRSYLG
ALKFMPHAVL KLLENMPMPW ESRRDVKVLY HTNGCLTLVN EVPRVIEPVF HAQWAAMWLA
MRKEKSDRRH FKRMRFPPFD DEEPPLSYSE NIEDVEPLEP IQLELDEDED RPVYEWFYEH
RPLLDTPHVN GPSYETWNLD LPQMATLYRL SKQLLSETVD KNYFHMFDMS SFQTAKALNV
AIPGGPRFEP LYKDIDPNDE DFGEFNAMDR IIFRSPIRTE YRVSYPYLYN SLPRSVKLAW
YSYPQVVYVR AEDPDLPAFY FDPGINPISS RAVAPKNLSV SHEDFVFGED DEDDEDDFQM
PEEMEPFMAD EELSTPETAS AIALWWAPHP FDKRSGRMVR AQDVPLVKQW YLEHVPAGQP
VKVRVSYQKL LKTFVLNELH KKPPQAQNKQ NLMRTLKSTK FFQQTKLDWV EAGLQVCRQG
YNMLNLLIHR KNLTYLHLDY NFNLKPVKTL TTKERKKSRF GNAFHLMREI LRLTKLIVDA
QVQYRLGNID AFQLADGILY AFNHVGQLTG MYRYKYKLMH QIRSCKDLKH LIYYRFNSGP
VGKGPGCGFW APAWRVWLFF MRGIIPLLER WLGNLLSRQF EGRHSKGVAK TVTKQRVESH
FDLELRAAVM SDLMDMMPEG IKQNKVNTVL QHLSEAWRCW KSNIPWKVPG LPAPIENIIL
RYVKSKADWW ISVAHYNRER IRRGATVDKT VAKKNLGRLT RLWLKAEQER QHNYMKDGPY
VSSEEAVAIY TTMVHWLEAR KFQPIPFPSV SYKHDTKILI LALERLREAY SVKGRLNQSQ
REELALIEQA YDSPGTTLAR IKRFLLTQRA FKEVGIDMND NYSNINPVYD IEPMEKITDA
YLDQYLWYQA DQRHLFPAWI KPSDSEVPPL LTYKWAQGIN NLDKVWESQD GECNVLIETQ
LSKVYEKIDL TMLNRLLRLI MDHNLADYIT AKNNVQLNYK DMNHVNAYGM IRGLQFSGFV
FQFYGLVLDI LLLGLQRAND IAGAPESPND FLQFKDKETE VRHPIRLYTR YIDRIWVFFR
FTAEESRDLI QRFLTENPDP NFENVIGYKN KKCWPRDSRM RLMRHDVNLG RAVFWDLKNR
LPRSVTTIEW EDTFASVYSR DNPNLLFSMN GFEVRILPKI RNTTGEFPTK DSVWSLVDNS
TKERTADAFL QVTEEDIQKF NNRIRQILMS SGSTTFTKIA NKWNTTLIAL FTYYREAAVS
TVNLLDTIVK CETKIQTRVK IGLNSKMPSR FPPAVFYTPK ELGGLGMISG SHILIPASDK
QWHKQTDTGI THFRAGMSHD EETLIPNIFR YIIPWESEFV DSQRVWMEYS QKRQEAQQQN
RRLTLEDLED SWDRGLPRIN TLFQKDRSTL SFDKGFRART EFKIYQHMKS NPFWWTSQRH
DGKLWALQDY RTDVIQALGG VETILHHTLF QATAFPSWEG LFWEKACLQN GTKLLRYDAT
EVAVEEVKDG DLLLGPDGGP RVVSNVVSGT DHLYRIKIGG RKEDLVVTSN HILVLHREKG
AGNVYAEPST EDHHYEEDDL LDTELDSVPI SASERYDTVE MTAQEFASVD PTIQSKYRLF
RCPGFDLPES DVPVHPYFLG LWLGDGSRTS TTIYSNHERE VREFLSNYAA ELDLHFVWHG
GLNYATVGRT RLADRPLPTV KEGKAPARPA VRHARQTIIK QRLAGGWTFQ ENRGPRELRL
WIRPGDEVCI DSICTSLSLT HVQEDDLNEY LDLIEAESAD EMSDDEDLSS DDVHIINHDT
GGRPSHYERQ RVTRLGAGRR VHGDLLPEEQ DELVNEIVEH DENATAENST GVNKLLVALR
KLGVMPPPGE TGHEVDRKHI PTAYMKNSRA VRLAVLAGLL DSDGWYVYPE NMFGFAQSAL
WHKTLFWNTV ALARSLGFSV WTTERDMWNP NRTKKTPHFF AQISGNLSEV PCLLTRKKAC
ERYIPQMHSF VIKDITLESS PTGWSGFKVD QDQLYLRHDY IVLHNSGFEE SMKFKKLTNA
QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF LHGKIPTLKI SLIQIFRAHL
WQKIHESVVM DLCQVFDQEL EPLGIETVQK ETIHPRKSYK MNSSCADILL FASHKWSVSP
PSMLHDTKDT MSATTTNKFW IDVQLRYGDF DSHNIERYVR AKYLDYTQDS MSIYPSATGL
MIAIDLAYNL YSAYGQYFPG LKQLIQQAMA KIMKANPALF VLRERIRKGL QLYASESSQE
FLNSQNYSEL FSNQIQLFID DTNVYRVTIH KTFEGNLTTK PINGAIFIFN PRTGQLFLKI
IHTSVWAGQK RLGQLAKWKT AEEVAALIRS LPVEEQPKQL IVTRKGLLDP LEVHLLDFPN
ISIRASELQL PFQAAMKVEK LGDMILRATG PQMVLFNLYD EWLKTISSYT AFSRLILILR
ALHVNQDKTK LLLRPDKTVI TQEHHIWPSL ADEDWVKVEV ELRDLILLDY GKKNNVNTSS
LTSSEVRDII LGMEISAPSM QRQQAAEIDK QQEEQQQLTA VTTKTQNVHG EEMVVTTTSQ
YEQASFASKT EWRTRAVATS NLRTRANNIY INSEDVKEED HFTYVMPKNV LKRFITIADL
RVQVAGYLYG KSPPDNNQVK EISTIVMIPQ VGNTRDVQLP KELPKHEYLE DLEPLGIIHT
VSGNEPPYMQ ASDVTQHARL MNRHASWDKK TVSMTVSFTP GSVSLAAWAL TPNGYKWGVE
NKDTMSDNPT GFSTSFGEKC QLLLSDKIRG YFLVPDNGIW NYSFMGSAFG TVEKKPVHVR
LDTPVRFYDA VHRPLHFHNF AELEDVWVDR ENQFE
//
