ID B2Z8E9_9INFA Unreviewed; 742 AA.
AC B2Z8E9;
DT 01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT 01-JUL-2008, sequence version 1.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:ACD12288.1};
OS Influenza A virus (A/duck/Thailand/CU-329/07(H5N1)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=519101 {ECO:0000313|EMBL:ACD12288.1};
RN [1] {ECO:0000313|EMBL:ACD12288.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/duck/Thailand/CU-329/07 {ECO:0000313|EMBL:ACD12288.1};
RX PubMed=18976558; DOI=10.3201/eid1411.080683;
RA Amonsin A., Choatrakol C., Lapkuntod J., Tantilertcharoen R.,
RA Thanawongnuwech R., Suradhat S., Suwannakan K., Theamboonlers A.,
RA Poovorawan Y.;
RT "Influenza virus (H5N1) in live bird markets and food markets, Thailand.";
RL Emerg. Infect. Dis. 14:1739-1742(2008).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; EU616849; ACD12288.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 3: Inferred from homology;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 283..480
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 49..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACD12288.1"
FT NON_TER 742
FT /evidence="ECO:0000313|EMBL:ACD12288.1"
SQ SEQUENCE 742 AA; 84611 MW; 7BD59DD28C8E25AA CRC64;
NPTLLFLKVP VQNAISTTFP YTGDPPYSHG TGTGYTMDTV NRTHQYSEKG KWTTNTETGA
PQLNPIDGPL PEDNEPSGYA QTDCVLEAMA FLEESHPGIF ENSCLETMEI VQQTRVDKLT
QGRQTYDWTL NRNQPAATAL ANTIEIFRSN GLTANESGRL IDFLKDVMES MDKEEMEMTT
HFQRKRRVRD NMTKKMVTQR TIGKKKQKLN KKSYLIRALT LNTMTKDAER GKLKRRAIAT
PGMQIRGFVY FVETLARSIC EKLEQSGLPV GGNEKKAKLA NVVRKMMTSS QDTELSFTIT
GDNTKWNENQ NPRMFLAMIT YITRNQPEWF RNVLSIAPIM FSNKMARLGK GYMFESKSMK
LRTQIPAEML ANIDLKYFNE ITKKKIEKIR PLLIDGTASL SPGMMMGMFN MLSTVLGVSI
LNLGQKRYTK TTYWWDGLQS SDDFALIVNA PNHEGIQAGV DRFYRTCKLV GINMSKKKSY
INRTGTFEFT SFFYRYGFVA NFSMELPSFG VSGINESADM SIGVTVIKNN MINNDLGPAT
AQMALQLFIK DYRYTYRCHR GDTQIQTRRS FELKKLWEQT RSKAGLLVSD GGPNLYNIRN
LHIPEVCLKW ELMDEDYQGR LCNPLNPFVS HKEIESVNNA VVMPAHGPAK SMEYDAVATT
HSWIPKRNRS ILNTSQRGIL EDEQMYQKCC NLFEKFFPSS SYRRPVGLSS MVEAMVSRAR
IDARIDFESG KIKKEEFAEI MK
//
