ID B3FWV1_MACNE Unreviewed; 555 AA.
AC B3FWV1;
DT 22-JUL-2008, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2008, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE SubName: Full=Perforin {ECO:0000313|EMBL:ACD40308.1, ECO:0000313|Ensembl:ENSMNEP00000005040.1};
OS Macaca nemestrina (Pig-tailed macaque).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9545 {ECO:0000313|EMBL:ACD40308.1};
RN [1] {ECO:0000313|EMBL:ACD40308.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=5821 {ECO:0000313|EMBL:ACD40308.1};
RX PubMed=18665802; DOI=10.1089/aid.2008.0081;
RA Rollman E., Turner S.J., Kedzierska K., Kent S.J.;
RT "Anti-SIV cytolytic molecules in pigtail macaques.";
RL AIDS Res. Hum. Retroviruses 24:1127-1131(2008).
RN [2] {ECO:0000313|Ensembl:ENSMNEP00000005040.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the complement C6/C7/C8/C9 family.
CC {ECO:0000256|ARBA:ARBA00009214}.
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DR EMBL; EU526085; ACD40308.1; -; mRNA.
DR RefSeq; NP_001292853.1; NM_001305924.1.
DR RefSeq; XP_011713252.1; XM_011714950.1.
DR SMR; B3FWV1; -.
DR STRING; 9545.ENSMNEP00000005040; -.
DR Ensembl; ENSMNET00000025141.1; ENSMNEP00000005040.1; ENSMNEG00000022959.1.
DR GeneID; 105466068; -.
DR KEGG; mni:105466068; -.
DR CTD; 5551; -.
DR GeneTree; ENSGT00530000063725; -.
DR OMA; LCKNALQ; -.
DR OrthoDB; 4221421at2759; -.
DR Proteomes; UP000233120; Unplaced.
DR Bgee; ENSMNEG00000022959; Expressed in spleen and 4 other cell types or tissues.
DR GO; GO:0044194; C:cytolytic granule; IEA:Ensembl.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0001772; C:immunological synapse; IEA:Ensembl.
DR GO; GO:0005509; F:calcium ion binding; IEA:Ensembl.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0140911; F:pore-forming activity; IEA:InterPro.
DR GO; GO:0022829; F:wide pore channel activity; IEA:Ensembl.
DR GO; GO:0002357; P:defense response to tumor cell; IEA:Ensembl.
DR GO; GO:0051607; P:defense response to virus; IEA:Ensembl.
DR GO; GO:0140507; P:granzyme-mediated programmed cell death signaling pathway; IEA:Ensembl.
DR GO; GO:0002418; P:immune response to tumor cell; IEA:Ensembl.
DR GO; GO:0001771; P:immunological synapse formation; IEA:Ensembl.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR GO; GO:0051712; P:positive regulation of killing of cells of another organism; IEA:Ensembl.
DR GO; GO:0051260; P:protein homooligomerization; IEA:Ensembl.
DR GO; GO:0017038; P:protein import; IEA:Ensembl.
DR GO; GO:0009306; P:protein secretion; IEA:Ensembl.
DR GO; GO:0071806; P:protein transmembrane transport; IEA:Ensembl.
DR GO; GO:0001913; P:T cell mediated cytotoxicity; IEA:Ensembl.
DR CDD; cd04032; C2_Perforin; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR020864; MACPF.
DR InterPro; IPR020863; MACPF_CS.
DR InterPro; IPR037300; Perforin-1_C2.
DR PANTHER; PTHR46096; PERFORIN-1; 1.
DR PANTHER; PTHR46096:SF3; PERFORIN-1; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF01823; MACPF; 1.
DR SMART; SM00239; C2; 1.
DR SMART; SM00457; MACPF; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS00279; MACPF_1; 1.
DR PROSITE; PS51412; MACPF_2; 1.
PE 2: Evidence at transcript level;
KW Cytolysis {ECO:0000256|ARBA:ARBA00022852};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Reference proteome {ECO:0000313|Proteomes:UP000233120};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..555
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5014567657"
FT DOMAIN 27..375
FT /note="MACPF"
FT /evidence="ECO:0000259|PROSITE:PS51412"
FT DOMAIN 397..514
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
SQ SEQUENCE 555 AA; 61225 MW; 9044DB23B20A7543 CRC64;
MAACLLLLGI LLLLLPLPVP APCHTATRSE CKRSHKFVPG AWLAGEGVDV TSLRRSGSFP
VDTQRFLRPD GTCTLCENAL QEGTLQRLPL ALTNWRAQGS GCQRHVTRAK VSSTEAVARD
AARSIRNDWK VGLDVTPHPA GNVHVSVAGS HSQAANFAAQ KTHQDQYSFS TDTVECRFYS
YHVVHTPPLH PDFKRALGDL PLHFNASTQP AYLRLISNYG THFIRAVELG GRISALTALR
TCELALEGLT DDEVDDCLNV EAQVNIGARG SSSAEAKACE EKKKKHKMTA SFHQTYRERH
SEVVGGHHTS INDLLFGSQA GPEQYSAWVN SLLGSPGLVD YSLEPLHVLL DSQDPRREAL
RRALSQYLTD RARWRDCSRP CPPGRQKSPR DPCQCVCHGS AVTTQDCCPR QRGLAQLEVT
FIQAWGLWGD WFTATDAYVK VFFGGQELRT NTQWSNNNPI WSMPLDFGDV LLATGGPLRL
QVWDQDSGWD DDLLGTCDQA PKSGSHEARC NLKHGHLKFR YHARCLPHLG GGTCLDYVPQ
MLLGEPPGNR SGAVW
//
