ID B3R3C1_CUPTR Unreviewed; 612 AA.
AC B3R3C1;
DT 02-SEP-2008, integrated into UniProtKB/TrEMBL.
DT 02-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=RALTA_A0832 {ECO:0000313|EMBL:CAQ68803.1};
OS Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CCUG 44338 / CIP
OS 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424)).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=977880 {ECO:0000313|EMBL:CAQ68803.1, ECO:0000313|Proteomes:UP000001692};
RN [1] {ECO:0000313|EMBL:CAQ68803.1, ECO:0000313|Proteomes:UP000001692}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17343 / BCRC 17206 / CCUG 44338 / CIP 107171 / LMG 19424 /
RC R1 {ECO:0000313|Proteomes:UP000001692};
RX PubMed=18490699; DOI=10.1101/gr.076448.108;
RA Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D.,
RA Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V.,
RA Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C.,
RA Masson-Boivin C.;
RT "Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and
RT comparative genomics of rhizobia.";
RL Genome Res. 18:1472-1483(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CU633749; CAQ68803.1; -; Genomic_DNA.
DR RefSeq; WP_012352140.1; NC_010528.1.
DR AlphaFoldDB; B3R3C1; -.
DR REBASE; 18842; M.CtaORF832P.
DR GeneID; 29763133; -.
DR KEGG; cti:RALTA_A0832; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_012122_0_0_4; -.
DR BioCyc; CTAI977880:RALTA_RS03995-MONOMER; -.
DR Proteomes; UP000001692; Chromosome 1.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:CAQ68803.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CAQ68803.1}.
FT DOMAIN 6..138
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 152..451
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 612 AA; 68764 MW; 796271C17F1EBF88 CRC64;
MNHQALSSFI WSVADLLRGN YKQSEYGRVI LPFTVLRRLD CVLAITKPAV LAEFEAKTQA
GINPDPFLQR KAKQSFYNVS PLDLVKLLGD QDHIRQNLYS YLQGFSASAR DIFERFDFHM
QVERLAKANL LYLVTEKFAN IDLHPDTVDN AQMGLVFEEL IRKFAEISNE TAGEHFTPRE
VIRLMVNLIF IEDSDVLTAG NAVVRTIYDP TAGTGGMLSV ADEFLREHNP SARLTMYGQE
LNDESYAICK ADMLIKGQDV GNIVAGNTLS DDGHGARKFD YMLSNPPFGV EWKKVEKAVR
QEHEQRGFDG RFGPGLPRVS DGSMLFLMHL LSKMRPAADG GSRFGIVLNG SPLFTGGAGS
GESEIRRYVL ENDLVEAIVG LPTDMFYNTG IATYVWILSN KKPEDRKGWV QLIDASSFWQ
KMRKSLGSKR KEMNDEQIAM VTRLFGDFVE AETATVLDAD GKEVGRYVVA ATAQPPEAPV
GGRVKLAPIS RIFRNEEFGY TTITVERPLR DENGQLMLGV KGKQKGKPQA DSSLRDTENV
PLDEEIDAYF VREVLPHAPD AWVDDDKSKV GYEIPFNRHF YVFEPPRDLH TIDEELKAVS
VNIMRMLEEL AE
//
