ID B3STG5_DROME Unreviewed; 1286 AA.
AC B3STG5;
DT 02-SEP-2008, integrated into UniProtKB/TrEMBL.
DT 02-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN Name=MLE {ECO:0000313|EMBL:ABV48864.1};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000313|EMBL:ABV48864.1};
RN [1] {ECO:0000313|EMBL:ABV48864.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Congo145 {ECO:0000313|EMBL:ABV48864.1};
RA Vermaak D., Malik H.S., Rodriguez M.A., Bayes J.J.;
RT "Dramatic episodes of positive selection drive the evolution of the dosage
RT compensation complex genes in Drosophila melanogaster.";
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000256|ARBA:ARBA00008792}.
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DR EMBL; EF682042; ABV48864.1; -; Genomic_DNA.
DR VEuPathDB; VectorBase:FBgn0002774; -.
DR ExpressionAtlas; B3STG5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0033679; F:3'-5' DNA/RNA helicase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0010468; P:regulation of gene expression; IEA:UniProt.
DR CDD; cd17972; DEXHc_DHX9; 1.
DR CDD; cd19854; DSRM_DHX9_rpt1; 1.
DR CDD; cd19855; DSRM_DHX9_rpt2; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 3.30.160.20; -; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044447; DHX9_DEXHc.
DR InterPro; IPR044445; DHX9_DSRM_1.
DR InterPro; IPR044446; DHX9_DSRM_2.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF119; ATP-DEPENDENT RNA HELICASE A; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00035; dsrm; 2.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00358; DSRM; 2.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF54768; dsRNA-binding domain-like; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS50137; DS_RBD; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00266}.
FT DOMAIN 2..70
FT /note="DRBM"
FT /evidence="ECO:0000259|PROSITE:PS50137"
FT DOMAIN 169..241
FT /note="DRBM"
FT /evidence="ECO:0000259|PROSITE:PS50137"
FT DOMAIN 394..560
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 639..813
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 80..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1165..1199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1262..1286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1286 AA; 143060 MW; ECDE263311413061 CRC64;
MDIKSFLYQF CAKSQIEPKF DIRQTGPKNR QRFLCEVRVE PNTYIGVGNS TNKKDAEKNA
CRDFVNYLVR VGKLNTNDVP ADAGASGGGP RTGLEGAGMA GGSGQQKRVF DGQSGPQDLG
EAYRPLNHDG GDGGNRYSVI DRIQEQRDMN EAEAFDVNAA IHGNWTIENA KERLNIYKQT
NNIRDDYKYT PVGPEHARSF LAELSIYVPA LNRTVTARES GSNKKSASKS CALSLVRQLF
HLNVIEPFSG TLKKKKDEQL KPYPVKLSPN LINKIDEVIK GLDLPVVNPR NIKIELDGPP
IPLIVNLSRI DSSQQDGEKR QESSVIPWAP PQANWNTWHA CNIDEGELAT TSIDDLSMDY
ERSLRDRRQN DNEYRQFLEF REKLPIAAMR SEILTAINDN PVVIIRGNTG CGKTTQIAQY
ILDDYICSGQ GGYANIYVTQ PRRISAISVA ERVARERCEQ LGDTVGYSVR FESVFPRPYG
AILFCTVGVL LRKLEAGLRG VSHIIVDEIH ERDVNSDFLL VILRDMVDTY PDLHVILMSA
TIDTTKFSKY FGVCPVLEVP GRAFPVQQFF LEDIIQMTDF VPSAESRRKR KEVEDEEQLL
SEDKDEAEIN YNKVCEDKYS QKTRNAMAML SESDVSFELL EALLMHIKSK NIPGAILVFL
PGWNLIFALM KFLQNTNIFG DTSQYQILPC HSQIPRDEQR KVFEPVPEGV TKIILSTNIA
ETSITIDDIV FVIDICKARM KLFTSHNNLT SYATVWASKT NLEQRKGRAG RVRPGFCFTL
CSRARFQALE DNLTPEMFRT PLHEMALTIK LLRLGSIHHF LSKALEPPPV DAVIEAEVLL
REMRCLDAND ELTPLGRLLA RLPIEPRLGK MMVLGAVFCC ADLMAIMASY SSTFSEVFSL
DIGQRRLANH QKALSGTKCS DHVAMIVASQ MWRREKQRGE HMEARFCDWK GLQMSTMNVI
WDAKQQLLDL LQQAGFPEEC MISHEVDERI DGDDPVLDVS LALLCLGLYP NICVHKEKRK
VLTTESKAAL LHKTSVNCSN LAVTFPYPFF VFGEKIRTRA VSCKQLSMVS PLQVILFGSR
KIDLAANNIV RVDNWLNFDI EPELAAKIGA LKPALEDLIT VACDNPSDIL RLEEPYAQLV
KVVKDLCVKS AGDFGLQRES GILPHQSRQF SDGGGPPKRG RFETGRFTNS SFGRRGNGRT
FGGGYGNNGG GYGNIGGGYG NNAGGYGNNG GYGNNGGGYR NNGGGYGNNG GGYGNNRGGF
GDSFESNRGS GGGFRNGDQG GRWGNF
//
