ID   B3T4F3_9ARCH            Unreviewed;       240 AA.
AC   B3T4F3;
DT   02-SEP-2008, integrated into UniProtKB/TrEMBL.
DT   02-SEP-2008, sequence version 1.
DT   27-MAR-2024, entry version 35.
DE   RecName: Full=Type II methyltransferase {ECO:0000256|RuleBase:RU362026};
DE            EC=2.1.1.113 {ECO:0000256|RuleBase:RU362026};
DE   AltName: Full=N-4 cytosine-specific methyltransferase {ECO:0000256|RuleBase:RU362026};
GN   ORFNames=ALOHA_HF4000ANIW133O4ctg2g41 {ECO:0000313|EMBL:ABZ07462.1};
OS   uncultured marine crenarchaeote HF4000_ANIW133O4.
OC   Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
OC   environmental samples.
OX   NCBI_TaxID=455574 {ECO:0000313|EMBL:ABZ07462.1};
RN   [1] {ECO:0000313|EMBL:ABZ07462.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=18580971; DOI=10.1038/ismej.2008.62;
RA   Konstantinidis K.T., Delong E.F.;
RT   "Genomic patterns of recombination, clonal divergence and environment in
RT   marine microbial populations.";
RL   ISME J. 2:1052-1065(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC         methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC         Evidence={ECO:0000256|RuleBase:RU362026};
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC       {ECO:0000256|ARBA:ARBA00006594, ECO:0000256|RuleBase:RU362026}.
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DR   EMBL; EU016600; ABZ07462.1; -; Genomic_DNA.
DR   AlphaFoldDB; B3T4F3; -.
DR   REBASE; 25651; M.UcrHFORFCP.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR002941; DNA_methylase_N4/N6.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR001091; RM_Methyltransferase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR   PANTHER; PTHR13370:SF33; TYPE II METHYLTRANSFERASE M.MJAV; 1.
DR   Pfam; PF01555; N6_N4_Mtase; 1.
DR   PRINTS; PR00508; S21N4MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000256|RuleBase:RU362026};
KW   Restriction system {ECO:0000256|ARBA:ARBA00022747,
KW   ECO:0000256|RuleBase:RU362026};
KW   S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU362026};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          9..228
FT                   /note="DNA methylase N-4/N-6"
FT                   /evidence="ECO:0000259|Pfam:PF01555"
SQ   SEQUENCE   240 AA;  28236 MW;  2A89E5A3A455B847 CRC64;
     MKAIPDEKID LVLTDPPFAI NFKAKKANYN RNASRVLSGY NEIKVEDYYN FTNAWMCEVY
     RVLKKSGSMY VFSGWNNLKD ILTALDDNGF TTINHIIWKY QFGVVTAKKF VTSHYHCLFV
     CKDDKKIKFF PYSRFKKNAK TSDGRSLHYK DKEDVWIINR EYWTGDDKTP TKLPAEIIKK
     ILQYSSEKMD LVLDPFLGSG QVAVISKMLG RRYLGFEIVK QYYDFANKRL QKNVYRLKKE
//