ID B3V9S1_PSETH Unreviewed; 329 AA.
AC B3V9S1;
DT 02-SEP-2008, integrated into UniProtKB/TrEMBL.
DT 02-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN Name=gyrB {ECO:0000313|EMBL:ACE63407.1};
OS Pseudonocardia thermophila.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Pseudonocardia.
OX NCBI_TaxID=1848 {ECO:0000313|EMBL:ACE63407.1};
RN [1] {ECO:0000313|EMBL:ACE63407.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 43832 {ECO:0000313|EMBL:ACE63407.1};
RG Chinese Society for Microbiology;
RA Yang L.-L., Zhi X.-Y., Li W.-J.;
RT "Phylogenetic relationships of Pseudonocardia species based on multilocus
RT sequence analysis.";
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; EU722579; ACE63407.1; -; Genomic_DNA.
DR AlphaFoldDB; B3V9S1; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 93..262
FT /note="DNA topoisomerase type IIA subunit B"
FT /evidence="ECO:0000259|Pfam:PF00204"
FT DOMAIN 291..327
FT /note="Toprim"
FT /evidence="ECO:0000259|Pfam:PF01751"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACE63407.1"
FT NON_TER 329
FT /evidence="ECO:0000313|EMBL:ACE63407.1"
SQ SEQUENCE 329 AA; 36660 MW; 9D79B4C2FFCF6E81 CRC64;
TYVRAVPGEL KRGEPTTRTG TSITFWADPE IFETTTYNVE TVRRRLQEMA FLNKGLTMIL
RDERNGDDGE AEEPDAEGYV AKVKEYKFHY PNGLEDFVAH LNKSKEPIHK KLIAYSAEEP
GHSLEVAMQW NSGYTESVYT FANTINTHEG GTHEEGFRAA LTSTVNRYAR EKKLLKDKDP
ALSGDDIREG LAAIVSVKLK EPQFEGQTKT KLGNTEVKSF VQRVSNEWLA DWLERNPTEA
KIIVNKAIQS AQARIAARKA RELVRRKAPG DIGGLPGKLA DCRSTDPSKC ELYIVEGDSA
GGSAKSGRDS MFQAILPIRG KIINVEKAR
//
