ID AMPA_PROMH Reviewed; 502 AA.
AC B4F2N1;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 01-MAY-2013, entry version 32.
DE RecName: Full=Probable cytosol aminopeptidase;
DE EC=3.4.11.1;
DE AltName: Full=Leucine aminopeptidase;
DE Short=LAP;
DE EC=3.4.11.10;
DE AltName: Full=Leucyl aminopeptidase;
GN Name=pepA; OrderedLocusNames=PMI3464;
OS Proteus mirabilis (strain HI4320).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Proteus.
OX NCBI_TaxID=529507;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HI4320;
RX PubMed=18375554; DOI=10.1128/JB.01981-07;
RA Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S.,
RA Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T.,
RA Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA Rabbinowitsch E., Walker D., Whithead S., Thomson N.R., Rather P.N.,
RA Parkhill J., Mobley H.L.T.;
RT "Complete genome sequence of uropathogenic Proteus mirabilis, a master
RT of both adherence and motility.";
RL J. Bacteriol. 190:4027-4037(2008).
CC -!- FUNCTION: Presumably involved in the processing and regular
CC turnover of intracellular proteins. Catalyzes the removal of
CC unsubstituted N-terminal amino acids from various peptides (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: Release of an N-terminal amino acid, Xaa-|-
CC Yaa-, in which Xaa is preferably Leu, but may be other amino acids
CC including Pro although not Arg or Lys, and Yaa may be Pro. Amino
CC acid amides and methyl esters are also readily hydrolyzed, but
CC rates on arylamides are exceedingly low.
CC -!- CATALYTIC ACTIVITY: Release of an N-terminal amino acid,
CC preferentially leucine, but not glutamic or aspartic acids.
CC -!- COFACTOR: Binds 2 manganese ions per subunit (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the peptidase M17 family.
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DR EMBL; AM942759; CAR46791.1; -; Genomic_DNA.
DR RefSeq; YP_002153148.1; NC_010554.1.
DR ProteinModelPortal; B4F2N1; -.
DR STRING; 529507.PMI3464; -.
DR PRIDE; B4F2N1; -.
DR EnsemblBacteria; CAR46791; CAR46791; PMI3464.
DR GeneID; 6801764; -.
DR KEGG; pmr:PMI3464; -.
DR PATRIC; 20521660; VBIProMir120933_3392.
DR eggNOG; COG0260; -.
DR HOGENOM; HOG000243132; -.
DR KO; K01255; -.
DR OMA; LMSVMEY; -.
DR ProtClustDB; PRK00913; -.
DR BioCyc; PMIR529507:GJIW-3549-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0008235; F:metalloexopeptidase activity; IEA:HAMAP.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR HAMAP; MF_00181; Cytosol_peptidase_M17; 1; -.
DR InterPro; IPR011356; Leucine_aapep/pepB.
DR InterPro; IPR000819; Peptidase_M17_C.
DR InterPro; IPR023042; Peptidase_M17_leu_NH2_pept.
DR InterPro; IPR008283; Peptidase_M17_N.
DR PANTHER; PTHR11963:SF3; PTHR11963:SF3; 1.
DR Pfam; PF00883; Peptidase_M17; 1.
DR Pfam; PF02789; Peptidase_M17_N; 1.
DR PRINTS; PR00481; LAMNOPPTDASE.
DR PROSITE; PS00631; CYTOSOL_AP; 1.
PE 3: Inferred from homology;
KW Aminopeptidase; Complete proteome; Cytoplasm; Hydrolase; Manganese;
KW Metal-binding; Protease.
FT CHAIN 1 502 Probable cytosol aminopeptidase.
FT /FTId=PRO_1000098336.
FT ACT_SITE 281 281 Potential.
FT ACT_SITE 355 355 Potential.
FT METAL 269 269 Manganese 2 (By similarity).
FT METAL 274 274 Manganese 1 (By similarity).
FT METAL 274 274 Manganese 2 (By similarity).
FT METAL 292 292 Manganese 2 (By similarity).
FT METAL 351 351 Manganese 1 (By similarity).
FT METAL 353 353 Manganese 1 (By similarity).
FT METAL 353 353 Manganese 2 (By similarity).
SQ SEQUENCE 502 AA; 54857 MW; 9E4E5738ACC06DF2 CRC64;
MEFNVKSGSP EKQRSACIIV GVFEPRRLSP IAEQLDKISD GYISALLRRG ELEGKVGQSL
LLHHVPNVLS ERVLLIGCGK ERELDERQYK QIIQKTINTL NETGSMEAVC FLTELHVKGR
NNYWKVRQAV ETAKDCLYTF DQLKSNKTEL RRPLRKMVFN VPTRRELPSG ERAIAHGLAI
ASGIKACKDL ANMPPNICNA AYLASQARQL ADSSANVSTR VIGEEQMKEL NMNAYLAVGQ
GSQNESLMSI IEYKGNQDPE SRPIVLVGKG LTFDSGGISI KPADGMDEMK YDMCGAATVY
GVMRVVAELQ LPINVIGVLA GCENMPGGKA YRPGDILTTM SGQTVEVLNT DAEGRLVLCD
TLTYVERFEP ELVIDIATLT GACMVALGHH YSGLMSNHNP LAHELMNASE QAGDRAWRLP
LGEEFYEQIE SNFADLANTG GRLGGAITAG CFLARFASKY NWAHLDIAGT AWRSGKAKGA
TGRPVSLLSQ FLLNRAGLNS DE
//
