ID   B4FIT6_MAIZE            Unreviewed;       565 AA.
AC   B4FIT6;
DT   23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT   23-SEP-2008, sequence version 1.
DT   27-MAR-2024, entry version 67.
DE   RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE            EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577 {ECO:0000313|EMBL:ACF82029.1};
RN   [1] {ECO:0000313|EMBL:ACF82029.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=B73 {ECO:0000313|EMBL:ACF82029.1};
RX   PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA   Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA   Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA   Walbot V., Yu Y.;
RT   "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL   PLoS Genet. 5:E1000740-E1000740(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC         ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC         Evidence={ECO:0000256|ARBA:ARBA00023501};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC       {ECO:0000256|ARBA:ARBA00007185}.
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DR   EMBL; BT037024; ACF82029.1; -; mRNA.
DR   RefSeq; NP_001304293.1; NM_001317364.1.
DR   AlphaFoldDB; B4FIT6; -.
DR   GeneID; 100216599; -.
DR   KEGG; zma:100216599; -.
DR   OrthoDB; 3266779at2759; -.
DR   ExpressionAtlas; B4FIT6; baseline and differential.
DR   GO; GO:0016836; F:hydro-lyase activity; IEA:UniProt.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043436; P:oxoacid metabolic process; IEA:UniProt.
DR   CDD; cd01580; AcnA_IRP_Swivel; 1.
DR   Gene3D; 6.10.190.10; -; 1.
DR   Gene3D; 3.30.499.10; Aconitase, domain 3; 1.
DR   Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR   InterPro; IPR044137; AcnA_IRP_Swivel.
DR   InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR   InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR006249; Aconitase/IRP2.
DR   InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR   InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR   PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR   Pfam; PF00330; Aconitase; 1.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   PRINTS; PR00415; ACONITASE.
DR   SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR   SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR   PROSITE; PS00450; ACONITASE_1; 1.
DR   PROSITE; PS01244; ACONITASE_2; 1.
PE   2: Evidence at transcript level;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT   DOMAIN          3..233
FT                   /note="Aconitase/3-isopropylmalate dehydratase large
FT                   subunit alpha/beta/alpha"
FT                   /evidence="ECO:0000259|Pfam:PF00330"
FT   DOMAIN          362..490
FT                   /note="Aconitase A/isopropylmalate dehydratase small
FT                   subunit swivel"
FT                   /evidence="ECO:0000259|Pfam:PF00694"
SQ   SEQUENCE   565 AA;  61817 MW;  C64B951B6D6C485E CRC64;
     MIEAYLRANK MFVDYNEPPT ERIYSSYLEL NLDEVEPSMS GPKRPHDRVP LKEMKSDWHA
     CLDNKVGFKG FAVPKEQQDK VVKFDFHGQP AEMKHGSVVI AAITSCTNTS NPSVMLGAGL
     VAKKACELGL EVKPWVKTSL APGSGVVTKY LLQSGLQEYL NQQGFHIVGY GCTTCIGNSG
     DLDESVSTAI TENDVVAAAV LSGNRNFEGR VHPLTRANYL ASPPLVVAYA LAGTVDIDFE
     KEPIGFGKDG KEVYFRDIWP STEEIAQVVQ SSVLPDMFKG TYEAITKGNP MWNQLTVPEA
     SLYSWDSKST YIHEPPYFKD MTMSPPGPST VKDAYCLLNF GDSITTDHIS PAGSIHKDSP
     AAKYLMERGV DRKDFNSYGS RRGNDEVMAR GTFANIRIVN KFLNGEVGPK TIHVPTGEKL
     SVFDAAMRYK SEGHATIILA GAEYGSGSSR DWAAKGPMLL GVKAVIAKSF ERIHRSNLVG
     MGIIPLCFKA GEDADSLGLT GHERYSIDLP TNLSEIRPGQ DVTVTTDNGK SFTCIVRFDT
     EVELAYFNHG GILPYVIRNL AAAHN
//