ID WUHO_DROVI Reviewed; 397 AA.
AC B4MA12;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GJ15762;
OS Drosophila virilis (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila.
OX NCBI_TaxID=7244;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson 15010-1051.87;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC 46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC induce conformational changes of the catalytic subunit. Required during
CC gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC at early stages, from germarium to stage 4 egg chambers. Also present
CC in the nuclei of spermatocytes and in the apical cells of the testes.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC Rule:MF_03056}.
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DR EMBL; CH940655; EDW66071.1; -; Genomic_DNA.
DR RefSeq; XP_002057963.1; XM_002057927.2.
DR AlphaFoldDB; B4MA12; -.
DR SMR; B4MA12; -.
DR STRING; 7244.B4MA12; -.
DR EnsemblMetazoa; FBtr0231687; FBpp0230179; FBgn0202950.
DR GeneID; 6634534; -.
DR KEGG; dvi:6634534; -.
DR eggNOG; KOG3914; Eukaryota.
DR HOGENOM; CLU_054270_0_0_1; -.
DR InParanoid; B4MA12; -.
DR OMA; SVWFKKR; -.
DR OrthoDB; 5480021at2759; -.
DR PhylomeDB; B4MA12; -.
DR UniPathway; UPA00989; -.
DR Proteomes; UP000008792; Unassembled WGS sequence.
DR GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR HAMAP; MF_03056; TRM82; 1.
DR InterPro; IPR028884; Trm82.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR16288:SF0; TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4; 1.
DR PANTHER; PTHR16288; WD40 REPEAT PROTEIN 4; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Developmental protein; Differentiation; Nucleus; Oogenesis;
KW Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT CHAIN 1..397
FT /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT subunit wuho"
FT /id="PRO_0000370549"
FT REPEAT 70..110
FT /note="WD 1"
FT REPEAT 114..153
FT /note="WD 2"
FT REPEAT 157..196
FT /note="WD 3"
FT REPEAT 200..239
FT /note="WD 4"
FT REPEAT 300..340
FT /note="WD 5"
SQ SEQUENCE 397 AA; 44543 MW; 55C9A00ABD0624DC CRC64;
MTTIFYAEPE LVLGHGRKVL FLNPNDLQIF KDIELPADLT TCGLKIAAGE TNEEQQQPVG
SEKAAAAGTN LEVSIVNVSY SPDRQLIALT TAGQKALLLY KSRPEHAKLL SVRALARASS
SLAFAPDSSS VLVTDKTGDC YRYDCVELEA PPQLLLGHLS IVYDILWTPD QKYIITCDRD
DKIRVTNYPA TYDIHSYCLG HKEFVSGLAL LTGTEHLVSA SGDKTLRVWN YLSGKQLLQH
ELPAPAVRLQ LRQLLPAKRF QVAVLFYDHV EALGLYELAL DEQSNDAWTI ARQQLVRAEA
GSWNISCFAL TADSIYVAGA LDERLTLRVY NSSDGEQAKT VPTGWLDMVT ERFADQTCVP
EDLSVWFKKR YDNVSDYMER KKRRIEDHQQ QQQQQKS
//
