ID B5DFS0_XENTR Unreviewed; 293 AA.
AC B5DFS0;
DT 14-OCT-2008, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2008, sequence version 1.
DT 22-FEB-2023, entry version 51.
DE RecName: Full=m7GpppX diphosphatase {ECO:0000256|ARBA:ARBA00015636};
DE EC=3.6.1.59 {ECO:0000256|ARBA:ARBA00012520};
DE AltName: Full=DCS-1 {ECO:0000256|ARBA:ARBA00032946};
DE AltName: Full=Decapping scavenger enzyme {ECO:0000256|ARBA:ARBA00030609};
DE AltName: Full=Hint-related 7meGMP-directed hydrolase {ECO:0000256|ARBA:ARBA00030789};
DE AltName: Full=Histidine triad nucleotide-binding protein 5 {ECO:0000256|ARBA:ARBA00030042};
DE AltName: Full=Histidine triad protein member 5 {ECO:0000256|ARBA:ARBA00030830};
DE AltName: Full=Scavenger mRNA-decapping enzyme DcpS {ECO:0000256|ARBA:ARBA00029885};
DE Flags: Fragment;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364 {ECO:0000313|EMBL:AAI69168.1};
RN [1] {ECO:0000313|EMBL:AAI69168.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=N6 {ECO:0000313|EMBL:AAI69168.1};
RC TISSUE=Intestine {ECO:0000313|EMBL:AAI69168.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside
CC in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + 2 H(+) +
CC N(7)-methyl-GMP; Xref=Rhea:RHEA:65388, Rhea:RHEA-COMP:17165,
CC Rhea:RHEA-COMP:17167, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58285, ChEBI:CHEBI:156461, ChEBI:CHEBI:167616;
CC EC=3.6.1.59; Evidence={ECO:0000256|ARBA:ARBA00024271};
CC -!- SUBUNIT: Homodimer. Associates with components of the exosome
CC multienzyme ribonuclease complex, such as EXOSC3 and EXOSC4. Interacts
CC with NDOR1. {ECO:0000256|ARBA:ARBA00011140}.
CC -!- SIMILARITY: Belongs to the HIT family. {ECO:0000256|ARBA:ARBA00010208}.
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DR EMBL; BC169168; AAI69168.1; -; mRNA.
DR AlphaFoldDB; B5DFS0; -.
DR GO; GO:0140932; F:5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IEA:InterPro.
DR Gene3D; 3.30.428.10; HIT-like; 1.
DR Gene3D; 3.30.200.40; Scavenger mRNA decapping enzyme, N-terminal domain; 1.
DR InterPro; IPR008594; DcpS/DCS2.
DR InterPro; IPR036265; HIT-like_sf.
DR InterPro; IPR011145; Scavenger_mRNA_decap_enz_N.
DR PANTHER; PTHR12978; HISTIDINE TRIAD HIT PROTEIN MEMBER; 1.
DR PANTHER; PTHR12978:SF0; M7GPPPX DIPHOSPHATASE; 1.
DR Pfam; PF05652; DcpS; 1.
DR Pfam; PF11969; DcpS_C; 1.
DR PIRSF; PIRSF028973; Scavenger_mRNA_decap_enz; 1.
DR SUPFAM; SSF54197; HIT-like; 1.
DR SUPFAM; SSF102860; mRNA decapping enzyme DcpS N-terminal domain; 1.
PE 2: Evidence at transcript level;
FT ACT_SITE 233
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-1"
FT BINDING 131
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT BINDING 141
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT BINDING 161
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT BINDING 163
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT BINDING 224..235
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR028973-2"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AAI69168.1"
SQ SEQUENCE 293 AA; 33966 MW; EDE0CA77DC3C14F1 CRC64;
PLSGFELKAV LRESARDKTI FLHGKVTSGN ENCDDAVIIL EKTPFQTDTV SSLLKNDPEL
KLQFKNDIYG VYQLSPPAEL NEIKTTVICP ASEKHIKKYL QQEVYLLHET EDDYKAITLP
YIESQSFSIQ WVYNILEKKA EAERIIYENP DPANGFVLIP DFKWNQKQVE DLYLIAICHQ
RGIRSLRDLN ADHLPLLMNI LEEGKEAILK RYGITGNKLR IYLHYQPSYY HLHVHFTVLG
HDAPGSSVER AHLLCDVIQN IESNPQYYQT RTLTFALRED EALLKKFKEL RDF
//
