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Database: UniProt
Entry: B5QZF1
LinkDB: B5QZF1
Original site: B5QZF1 
ID   RLMKL_SALEP             Reviewed;         702 AA.
AC   B5QZF1;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   04-NOV-2008, sequence version 1.
DT   29-OCT-2014, entry version 46.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase K/L {ECO:0000255|HAMAP-Rule:MF_01858};
DE   Includes:
DE     RecName: Full=23S rRNA m2G2445 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01858};
DE              EC=2.1.1.173 {ECO:0000255|HAMAP-Rule:MF_01858};
DE     AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmL {ECO:0000255|HAMAP-Rule:MF_01858};
DE   Includes:
DE     RecName: Full=23S rRNA m7G2069 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01858};
DE              EC=2.1.1.264 {ECO:0000255|HAMAP-Rule:MF_01858};
DE     AltName: Full=rRNA (guanine-N(7)-)-methyltransferase RlmK {ECO:0000255|HAMAP-Rule:MF_01858};
GN   Name=rlmL {ECO:0000255|HAMAP-Rule:MF_01858};
GN   OrderedLocusNames=SEN0926;
OS   Salmonella enteritidis PT4 (strain P125109).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=550537;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=P125109;
RX   PubMed=18583645; DOI=10.1101/gr.077404.108;
RA   Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S.,
RA   Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A.,
RA   Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B.,
RA   Ormond D., Abdellah Z., Brooks K., Cherevach I., Chillingworth T.,
RA   Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K.,
RA   Moule S., Mungall K., Saunders M., Whitehead S., Chabalgoity J.A.,
RA   Maskell D., Humphreys T., Roberts M., Barrow P.A., Dougan G.,
RA   Parkhill J.;
RT   "Comparative genome analysis of Salmonella enteritidis PT4 and
RT   Salmonella gallinarum 287/91 provides insights into evolutionary and
RT   host adaptation pathways.";
RL   Genome Res. 18:1624-1637(2008).
CC   -!- FUNCTION: Specifically methylates the guanine in position 2445
CC       (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA.
CC       {ECO:0000255|HAMAP-Rule:MF_01858}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(2445) in 23S
CC       rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_01858}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(2069) in 23S
CC       rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_01858}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01858}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmKL
CC       family. {ECO:0000255|HAMAP-Rule:MF_01858}.
CC   -!- SIMILARITY: Contains 1 THUMP domain. {ECO:0000255|HAMAP-
CC       Rule:MF_01858}.
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DR   EMBL; AM933172; CAR32509.1; -; Genomic_DNA.
DR   RefSeq; YP_002243057.1; NC_011294.1.
DR   ProteinModelPortal; B5QZF1; -.
DR   STRING; 550537.SEN0926; -.
DR   PRIDE; B5QZF1; -.
DR   EnsemblBacteria; CAR32509; CAR32509; SEN0926.
DR   PATRIC; 32332300; VBISalEnt14964_0939.
DR   eggNOG; COG0116; -.
DR   HOGENOM; HOG000218371; -.
DR   OMA; NKRGFKM; -.
DR   OrthoDB; EOG6HJ238; -.
DR   BioCyc; SENT550537:GJFI-938-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-HAMAP.
DR   GO; GO:0052915; F:23S rRNA (guanine(2445)-N(2))-methyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0070043; F:rRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.40.50.150; -; 3.
DR   HAMAP; MF_01858; 23SrRNA_methyltr_KL; 1.
DR   InterPro; IPR017244; 23SrRNA_methyltr_KL.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR002296; N12N6_MeTrfase.
DR   InterPro; IPR000241; RNA_methylase_dom.
DR   InterPro; IPR019614; SAM-dep_methyl-trfase.
DR   InterPro; IPR029063; SAM-dependent_MTases-like.
DR   InterPro; IPR004114; THUMP.
DR   Pfam; PF10672; Methyltrans_SAM; 1.
DR   Pfam; PF02926; THUMP; 1.
DR   Pfam; PF01170; UPF0020; 1.
DR   PIRSF; PIRSF037618; RNA_Mtase_bacteria_prd; 1.
DR   PRINTS; PR00507; N12N6MTFRASE.
DR   SMART; SM00981; THUMP; 1.
DR   SUPFAM; SSF53335; SSF53335; 3.
DR   PROSITE; PS51165; THUMP; 1.
DR   PROSITE; PS01261; UPF0020; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Methyltransferase; RNA-binding;
KW   rRNA processing; S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    702       Ribosomal RNA large subunit
FT                                methyltransferase K/L.
FT                                /FTId=PRO_0000366808.
FT   DOMAIN       43    154       THUMP. {ECO:0000255|HAMAP-Rule:MF_01858}.
SQ   SEQUENCE   702 AA;  78849 MW;  031E82BCA744E152 CRC64;
     MNSLFASTAR GLEELLKTEL EKLGAVGCQV VQGGVHFQGD TRLIYQSLMW SRLASRIILP
     MGECKVYSDL DLYLGVQAIN WTEIFNPGAT FAVHFSGLND TIRNSQYGAM KVKDAIVDAF
     TRKNLPRPNV DRESPDLRIN VWLNKETASI ALDLSGDGLH LRGYRDRTGL APIKETLAAA
     IVMRSGWQPG TPLLDPMCGS GTLLIEAAMW ATDRAPGLHR GHWGFSGWAQ HDETIWQEVK
     AEAQTRARKG LAEYSSHFYG SDSDARVIER ARSNARRAGI GELITFEVKD VAQLSNPLPK
     GPYGTVISNP PYGERLDSEP ALIALHSLLG RTMKNQFGGW NLSLFSASPD LLGSLQLRAD
     KQFKAKNGPL DCVQKNYHIA ETTADSKPAT VAEDYANRLR KNLKKLEKWA RQEGIECYRL
     YDADLPEYNV AVDRYGDWAV IQEYAPPKTV DAQKARQRLF DIIAATLSVL GIPPNKLVLK
     TRERQKGKNQ YQKMSEKGEF LEVSEYNARL WVNLTDYLDT GLFLDHRIAR RMLGEMSKGK
     DFLNLFSYTG SASVHAGLGG ARSTTTVDMS RTYLEWAERN LRLNGLSGRA HRLIQADCLG
     WLREANEQFD LIFIDPPTFS NSKRMEESFD VQRDHVALMK DLKRLLRKGG TIMFSNNKRG
     FRMDLEGLAE LGLTAQEITQ KTLSPDFARN RQIHNCWLIR AA
//
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