ID RLMKL_SALEP Reviewed; 702 AA.
AC B5QZF1;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-NOV-2008, sequence version 1.
DT 01-MAY-2013, entry version 37.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase K/L;
DE Includes:
DE RecName: Full=23S rRNA m2G2445 methyltransferase;
DE EC=2.1.1.173;
DE AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmL;
DE Includes:
DE RecName: Full=23S rRNA m7G2069 methyltransferase;
DE EC=2.1.1.264;
DE AltName: Full=rRNA (guanine-N(7)-)-methyltransferase RlmK;
GN Name=rlmL; OrderedLocusNames=SEN0926;
OS Salmonella enteritidis PT4 (strain P125109).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=550537;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P125109;
RX PubMed=18583645; DOI=10.1101/gr.077404.108;
RA Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S.,
RA Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A.,
RA Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B.,
RA Ormond D., Abdellah Z., Brooks K., Cherevach I., Chillingworth T.,
RA Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K.,
RA Moule S., Mungall K., Saunders M., Whitehead S., Chabalgoity J.A.,
RA Maskell D., Humphreys T., Roberts M., Barrow P.A., Dougan G.,
RA Parkhill J.;
RT "Comparative genome analysis of Salmonella enteritidis PT4 and
RT Salmonella gallinarum 287/91 provides insights into evolutionary and
RT host adaptation pathways.";
RL Genome Res. 18:1624-1637(2008).
CC -!- FUNCTION: Specifically methylates the guanine in position 2445
CC (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA
CC (By similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(2445) in 23S
CC rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S
CC rRNA.
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(2069) in 23S
CC rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S
CC rRNA.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmKL
CC family.
CC -!- SIMILARITY: Contains 1 THUMP domain.
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DR EMBL; AM933172; CAR32509.1; -; Genomic_DNA.
DR RefSeq; YP_002243057.1; NC_011294.1.
DR ProteinModelPortal; B5QZF1; -.
DR STRING; 550537.SEN0926; -.
DR PRIDE; B5QZF1; -.
DR GeneID; 6947594; -.
DR KEGG; set:SEN0926; -.
DR PATRIC; 32332300; VBISalEnt14964_0939.
DR eggNOG; COG0116; -.
DR HOGENOM; HOG000218371; -.
DR KO; K12297; -.
DR OMA; NKRGFKM; -.
DR ProtClustDB; PRK11783; -.
DR BioCyc; SENT550537:GJFI-938-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0052915; F:23S rRNA (guanine(2445)-N(2))-methyltransferase activity; IEA:HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:HAMAP.
DR GO; GO:0070043; F:rRNA (guanine-N7-)-methyltransferase activity; IEA:HAMAP.
DR GO; GO:0070476; P:rRNA (guanine-N7)-methylation; IEA:GOC.
DR HAMAP; MF_01858; 23SrRNA_methyltr_KL; 1; -.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR002296; N12N6_MeTrfase.
DR InterPro; IPR000241; RNA_methylase_dom.
DR InterPro; IPR017244; S-Ado-dep_RNA_MeTrfase_bac.
DR InterPro; IPR019614; SAM-dep_methyl-trfase.
DR InterPro; IPR004114; THUMP.
DR Pfam; PF10672; Methyltrans_SAM; 1.
DR Pfam; PF02926; THUMP; 1.
DR Pfam; PF01170; UPF0020; 1.
DR PIRSF; PIRSF037618; RNA_Mtase_bacteria_prd; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SMART; SM00981; THUMP; 1.
DR PROSITE; PS51165; THUMP; 1.
DR PROSITE; PS01261; UPF0020; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Methyltransferase; RNA-binding;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1 702 Ribosomal RNA large subunit
FT methyltransferase K/L.
FT /FTId=PRO_0000366808.
FT DOMAIN 43 154 THUMP.
SQ SEQUENCE 702 AA; 78849 MW; 031E82BCA744E152 CRC64;
MNSLFASTAR GLEELLKTEL EKLGAVGCQV VQGGVHFQGD TRLIYQSLMW SRLASRIILP
MGECKVYSDL DLYLGVQAIN WTEIFNPGAT FAVHFSGLND TIRNSQYGAM KVKDAIVDAF
TRKNLPRPNV DRESPDLRIN VWLNKETASI ALDLSGDGLH LRGYRDRTGL APIKETLAAA
IVMRSGWQPG TPLLDPMCGS GTLLIEAAMW ATDRAPGLHR GHWGFSGWAQ HDETIWQEVK
AEAQTRARKG LAEYSSHFYG SDSDARVIER ARSNARRAGI GELITFEVKD VAQLSNPLPK
GPYGTVISNP PYGERLDSEP ALIALHSLLG RTMKNQFGGW NLSLFSASPD LLGSLQLRAD
KQFKAKNGPL DCVQKNYHIA ETTADSKPAT VAEDYANRLR KNLKKLEKWA RQEGIECYRL
YDADLPEYNV AVDRYGDWAV IQEYAPPKTV DAQKARQRLF DIIAATLSVL GIPPNKLVLK
TRERQKGKNQ YQKMSEKGEF LEVSEYNARL WVNLTDYLDT GLFLDHRIAR RMLGEMSKGK
DFLNLFSYTG SASVHAGLGG ARSTTTVDMS RTYLEWAERN LRLNGLSGRA HRLIQADCLG
WLREANEQFD LIFIDPPTFS NSKRMEESFD VQRDHVALMK DLKRLLRKGG TIMFSNNKRG
FRMDLEGLAE LGLTAQEITQ KTLSPDFARN RQIHNCWLIR AA
//
