ID B6A066_RHILW Unreviewed; 438 AA.
AC B6A066;
DT 25-NOV-2008, integrated into UniProtKB/TrEMBL.
DT 25-NOV-2008, sequence version 1.
DT 27-MAR-2024, entry version 94.
DE SubName: Full=Glycoside hydrolase family 4 {ECO:0000313|EMBL:ACI58000.1};
GN OrderedLocusNames=Rleg2_4755 {ECO:0000313|EMBL:ACI58000.1};
OS Rhizobium leguminosarum bv. trifolii (strain WSM2304).
OG Plasmid pRLG201 {ECO:0000313|EMBL:ACI58000.1,
OG ECO:0000313|Proteomes:UP000008330}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=395492 {ECO:0000313|EMBL:ACI58000.1, ECO:0000313|Proteomes:UP000008330};
RN [1] {ECO:0000313|EMBL:ACI58000.1, ECO:0000313|Proteomes:UP000008330}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WSM2304 {ECO:0000313|EMBL:ACI58000.1,
RC ECO:0000313|Proteomes:UP000008330};
RC PLASMID=pRLG201 {ECO:0000313|EMBL:ACI58000.1};
RX PubMed=21304679; DOI=10.4056/sigs.44642;
RA Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K., Tiwari R.,
RA Malfatti S., Kiss H., Lapidus A., Copeland A., Nolan M., Land M.,
RA Ivanova N., Mavromatis K., Markowitz V., Kyrpides N., Melino V., Denton M.,
RA Yates R., Howieson J.;
RT "Complete genome sequence of Rhizobium leguminosarum bv trifolii strain
RT WSM2304, an effective microsymbiont of the South American clover Trifolium
RT polymorphum.";
RL Stand. Genomic Sci. 2:66-76(2010).
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|RuleBase:RU361152};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
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DR EMBL; CP001192; ACI58000.1; -; Genomic_DNA.
DR RefSeq; WP_003592140.1; NC_011368.1.
DR AlphaFoldDB; B6A066; -.
DR CAZy; GH4; Glycoside Hydrolase Family 4.
DR KEGG; rlt:Rleg2_4755; -.
DR eggNOG; COG1486; Bacteria.
DR HOGENOM; CLU_045951_1_1_5; -.
DR Proteomes; UP000008330; Plasmid pRLG201.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd05297; GH4_alpha_glucosidase_galactosidase; 1.
DR Gene3D; 3.90.1820.10; AglA-like glucosidase; 1.
DR InterPro; IPR001088; Glyco_hydro_4.
DR InterPro; IPR022616; Glyco_hydro_4_C.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR32092:SF6; ALPHA-GALACTOSIDASE; 1.
DR Pfam; PF02056; Glyco_hydro_4; 1.
DR Pfam; PF11975; Glyco_hydro_4C; 1.
DR PRINTS; PR00732; GLHYDRLASE4.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Cobalt {ECO:0000256|PIRSR:PIRSR601088-3};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW Iron {ECO:0000256|PIRSR:PIRSR601088-3};
KW Manganese {ECO:0000256|PIRSR:PIRSR601088-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601088-3};
KW NAD {ECO:0000256|RuleBase:RU361152};
KW Nickel {ECO:0000256|PIRSR:PIRSR601088-3};
KW Plasmid {ECO:0000313|EMBL:ACI58000.1}.
FT DOMAIN 197..410
FT /note="Glycosyl hydrolase family 4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11975"
FT BINDING 149
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT BINDING 171
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT BINDING 201
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT SITE 111
FT /note="Increases basicity of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-4"
SQ SEQUENCE 438 AA; 48670 MW; 8336C07BDC9F2BAE CRC64;
MPKICLIGAG STVFAQNILG DVLSTPSDHD YIIGLFDIDP ERLKTSEIVA RRICASLKLD
TVRIEATLNR REALKGADFV ILMMQVGGYK PATVTDFNVA KNYGLRQTIA DTLGIGGIFR
GLRTIPVLES ICGDMEEVCP NALLMQYVNP MAINCWAIKE IAPSIRTVGL CHSVQHTADH
LARCLGEKID NISYISAGIN HIAFFLKYEK LHGDGSREDL YPKLKALAAE GKVPADDRVR
FDALKRLGHF VTESSEHFAE YTSWYIKNHQ PELVDQLNIP LDEYIRRCEL QISQWHVLRQ
DLEGGRPIEV CRSNEYASGI IHAAVTGKPA LIYGNVPNNG LIENLPPECI VEVPCHVDRN
GVQPTRIGRI PSQLAAVMRL SISVQELTVE AALTGKRDRI YQAALLDPHT SAELSPDKIW
HMVDDLIEAH GDLLPNYH
//
