ID B6K2T4_SCHJY Unreviewed; 1034 AA.
AC B6K2T4;
DT 16-DEC-2008, integrated into UniProtKB/TrEMBL.
DT 16-DEC-2008, sequence version 1.
DT 27-MAR-2024, entry version 79.
DE RecName: Full=Eukaryotic translation initiation factor 5B {ECO:0000256|ARBA:ARBA00013824};
DE AltName: Full=Translation initiation factor IF-2 {ECO:0000256|ARBA:ARBA00032478};
GN Name=tif52 {ECO:0000313|JaponicusDB:SJAG_03732};
GN ORFNames=SJAG_03732 {ECO:0000313|EMBL:EEB08574.1};
OS Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=402676 {ECO:0000313|EMBL:EEB08574.1, ECO:0000313|Proteomes:UP000001744};
RN [1] {ECO:0000313|EMBL:EEB08574.1, ECO:0000313|Proteomes:UP000001744}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=yFS275 / FY16936 {ECO:0000313|Proteomes:UP000001744};
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000256|ARBA:ARBA00007733}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KE651167; EEB08574.1; -; Genomic_DNA.
DR RefSeq; XP_002174867.1; XM_002174831.2.
DR AlphaFoldDB; B6K2T4; -.
DR STRING; 402676.B6K2T4; -.
DR EnsemblFungi; EEB08574; EEB08574; SJAG_03732.
DR GeneID; 7052248; -.
DR JaponicusDB; SJAG_03732; tif52.
DR VEuPathDB; FungiDB:SJAG_03732; -.
DR eggNOG; KOG1144; Eukaryota.
DR HOGENOM; CLU_002656_1_0_1; -.
DR OMA; EFAVMLC; -.
DR OrthoDB; 169393at2759; -.
DR Proteomes; UP000001744; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IEA:EnsemblFungi.
DR GO; GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:EnsemblFungi.
DR GO; GO:0005525; F:GTP binding; IEA:EnsemblFungi.
DR GO; GO:0003924; F:GTPase activity; IEA:EnsemblFungi.
DR GO; GO:0043022; F:ribosome binding; IEA:EnsemblFungi.
DR GO; GO:0070181; F:small ribosomal subunit rRNA binding; IEA:EnsemblFungi.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0031369; F:translation initiation factor binding; IEA:EnsemblFungi.
DR GO; GO:0042256; P:cytosolic ribosome assembly; IEA:EnsemblFungi.
DR GO; GO:0001732; P:formation of cytoplasmic translation initiation complex; IEA:EnsemblFungi.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR GO; GO:0006446; P:regulation of translational initiation; IEA:EnsemblFungi.
DR GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR CDD; cd03703; aeIF5B_II; 1.
DR CDD; cd01887; IF2_eIF5B; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR Gene3D; 3.40.50.10050; Translation initiation factor IF- 2, domain 3; 1.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43381:SF4; EUKARYOTIC TRANSLATION INITIATION FACTOR 5B; 1.
DR PANTHER; PTHR43381; TRANSLATION INITIATION FACTOR IF-2-RELATED; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF52156; Initiation factor IF2/eIF5b, domain 3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Initiation factor {ECO:0000256|ARBA:ARBA00022540,
KW ECO:0000313|EMBL:EEB08574.1};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000001744}.
FT DOMAIN 437..655
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
FT REGION 1..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..151
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..181
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..276
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 318..349
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..367
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 385..422
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1034 AA; 114983 MW; 661A10DFA130BEE4 CRC64;
MGKKGKKNAY ADWEDDLGTD ISGQNAYKEP EPVEEEVEEK MEKLDLTETA PSKKKKGKKG
KKNTPSPPVE EELEEPEEPV KTGPVELTAG VISEDEFAPI KPKKNKKKKA NKVVEPVEEE
VDVAVPEIRI KSKKEKERER KEREKLRKKQ QQAAKKGTPS SQTPVTGTSS PLDSSATEES
ATPKEAPVKT GKGKRKGPNV AALQKMLEQQ RAREEELKRE KEEEERRIAE EQRRLEEEER
KKEEAKQRKK EKEKRKKEEL KAQGKYLTKK QKEAMALAER RKKQMLEAGY VVAGLAEKAE
GEKKEKKKKK PVYDNRKRSG KSNASSASSS VILTPTTTEA QTPSETPASP VPSKLKEEEK
AAEDEAMFDS WEAALDEPEP ASWEEQLQEE EKPTEDSKSS EDSKAKAKDG KKETKNDVDA
IPEAEGNTDS NVSSSNMRSP ICCILGHVDT GKTKLLDNLR RSNVQEGEAG GITQQIGATY
FPIDALKEKV KVMEKESKID YQIPGLLIID TPGHESFTNL RSRGSSLCNI AILVIDIMHG
LEPQTIESIR LLRERKTPFI VALNKVDRLY GWHSIKDNAF QDSLKKQKKA IQREFQDRVK
SVILQLNEQG LNAALYYENK NMGRYVSLVP TSAVSGEGVP DLLKLLITLT QSRMSENLKY
LSKLECTVLE VKVIEGLGAT VDVVLSNGVL REGDRIILCG MSGPIITTVR ALLTPQPLKE
MRIKSAYVHH KEIKAAMGVK ICANDLDKAV AGSRLLVVGP DDDEEELADE IMEDLEDLLG
RIDTSGVGVS VQASTLGSLE ALLEFLKQME IPVSSVSVGP VYKKDIMRSA TMLEKAPEYA
IMLCFDVKID KDAEELAEQM GIKIFSANII YHLFDAFTAH QKQLMEKKRE EGKGVAVFPC
VLRTVAAFNK RDPIILGVDV VEGILRTGTP IVAVKPGPDG ENQVVELGTV ASIESNHKPV
EKVKKGQAGA GVAVKLESNG SQILFGRQVD EKDMLYSHIS RRSIDALKDP AFRNEVSREE
WQLIIQLKKV FGII
//
