GenomeNet

Database: UniProt
Entry: B7IUP8
LinkDB: B7IUP8
Original site: B7IUP8 
ID   PYRC_BACC2              Reviewed;         428 AA.
AC   B7IUP8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   26-NOV-2014, entry version 44.
DE   RecName: Full=Dihydroorotase {ECO:0000255|HAMAP-Rule:MF_00220};
DE            Short=DHOase {ECO:0000255|HAMAP-Rule:MF_00220};
DE            EC=3.5.2.3 {ECO:0000255|HAMAP-Rule:MF_00220};
GN   Name=pyrC {ECO:0000255|HAMAP-Rule:MF_00220};
GN   OrderedLocusNames=BCG9842_B1255;
OS   Bacillus cereus (strain G9842).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=405531;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=G9842;
RA   Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A.,
RA   Ravel J., Sutton G.;
RT   "Genome sequence of Bacillus cereus G9842.";
RL   Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC       aspartate. {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00220};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_00220};
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
CC       {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00220}.
CC   -!- SIMILARITY: Belongs to the DHOase family. Type 2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00220}.
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DR   EMBL; CP001186; ACK93450.1; -; Genomic_DNA.
DR   RefSeq; YP_002447453.1; NC_011772.1.
DR   ProteinModelPortal; B7IUP8; -.
DR   STRING; 405531.BCG9842_B1255; -.
DR   EnsemblBacteria; ACK93450; ACK93450; BCG9842_B1255.
DR   GeneID; 7184252; -.
DR   KEGG; bcg:BCG9842_B1255; -.
DR   PATRIC; 18903506; VBIBacCer50903_3905.
DR   eggNOG; COG0044; -.
DR   HOGENOM; HOG000219142; -.
DR   KO; K01465; -.
DR   OMA; HCEDTNL; -.
DR   OrthoDB; EOG6KHFW6; -.
DR   BioCyc; BCER405531:GI5L-4009-MONOMER; -.
DR   UniPathway; UPA00070; UER00117.
DR   GO; GO:0004151; F:dihydroorotase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.40.10; -; 1.
DR   HAMAP; MF_00220_B; PyrC_type2_B; 1.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR004722; DHOase.
DR   InterPro; IPR002195; Dihydroorotase_CS.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   TIGRFAMs; TIGR00857; pyrC_multi; 1.
DR   PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR   PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW   Zinc.
FT   CHAIN         1    428       Dihydroorotase.
FT                                /FTId=PRO_1000193094.
FT   METAL        59     59       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL        61     61       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       141    141       Zinc 1; via carbamate group.
FT                                {ECO:0000255|HAMAP-Rule:MF_00220}.
FT   METAL       141    141       Zinc 2; via carbamate group.
FT                                {ECO:0000255|HAMAP-Rule:MF_00220}.
FT   METAL       178    178       Zinc 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       231    231       Zinc 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   METAL       304    304       Zinc 1. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
FT   MOD_RES     141    141       N6-carboxylysine. {ECO:0000255|HAMAP-
FT                                Rule:MF_00220}.
SQ   SEQUENCE   428 AA;  46656 MW;  3C0BAFD189C457F5 CRC64;
     MNYLFKNGRY MNEEGKIVAT DLLVQDGKIA KVAENITADN AEVIEVNGNL IAPGLVDVHV
     HLREPGGEHK ETIETGTLAA AKGGFTTICS MPNTRPVPDC REHMEDLQKR IKEKAHVNVL
     PYGAITVRQA GSEMTDFETL KELGAFAFTD DGVGVQDASM MLAAMKRAAK LNMAVVAHCE
     ENTLINKGCV HEGKFSEKHG LNGIPSVCES VHIARDILLA EAADCHYHVC HVSTKGSVRV
     IRDAKRAGIK VTAEVTPHHL VLCEDDIPSV DPNFKMNPPL RGKEDHAALI EGLLDGTIDM
     IATDHAPHTA EEKAQGIERA PFGITGFETA FPLLYTNLVK KGIITLEQLI QFLTEKPADT
     FGLEAGRLKE GRTADITIID LEQEEEIDPT TFLSKGKNTP FAGWKCQGWP VMTIVGGKIA
     WQKESALV
//
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