ID ISPF_BACC0 Reviewed; 158 AA.
AC B7JK94;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 01-MAY-2013, entry version 37.
DE RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;
DE Short=MECDP-synthase;
DE Short=MECPP-synthase;
DE Short=MECPS;
DE EC=4.6.1.12;
GN Name=ispF; OrderedLocusNames=BCAH820_0097;
OS Bacillus cereus (strain AH820).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=405535;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AH820;
RA Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A.,
RA Ravel J., Sutton G.;
RT "Genome sequence of Bacillus cereus AH820.";
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of isopentenyl diphosphate
CC (IPP) and dimethylallyl diphosphate (DMAPP), two major building
CC blocks of isoprenoid compounds. Catalyzes the conversion of 4-
CC diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to
CC 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a
CC corresponding release of cytidine 5-monophosphate (CMP) (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: 2-phospho-4-(cytidine 5'-diphospho)-2-C-
CC methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate
CC + CMP.
CC -!- COFACTOR: Binds 1 divalent metal cation per subunit (By
CC similarity).
CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate
CC biosynthesis via DXP pathway; isopentenyl diphosphate from 1-
CC deoxy-D-xylulose 5-phosphate: step 4/6.
CC -!- SUBUNIT: Homotrimer (By similarity).
CC -!- SIMILARITY: Belongs to the IspF family.
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DR EMBL; CP001283; ACK88782.1; -; Genomic_DNA.
DR RefSeq; YP_002449123.1; NC_011773.1.
DR ProteinModelPortal; B7JK94; -.
DR SMR; B7JK94; 3-158.
DR STRING; 405535.BCAH820_0097; -.
DR EnsemblBacteria; ACK88782; ACK88782; BCAH820_0097.
DR GeneID; 7190338; -.
DR KEGG; bcu:BCAH820_0097; -.
DR PATRIC; 18836995; VBIBacCer122868_0095.
DR eggNOG; COG0245; -.
DR HOGENOM; HOG000239175; -.
DR KO; K01770; -.
DR OMA; HKFGGEG; -.
DR ProtClustDB; PRK00084; -.
DR BioCyc; BCER405535:GHSL-1955-MONOMER; -.
DR UniPathway; UPA00056; UER00095.
DR GO; GO:0008685; F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity; IEA:HAMAP.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:UniProtKB-UniPathway.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
DR Gene3D; 3.30.1330.50; -; 1.
DR HAMAP; MF_00107; IspF; 1; -.
DR InterPro; IPR003526; MECDP_synthase.
DR InterPro; IPR020555; MECDP_synthase_CS.
DR Pfam; PF02542; YgbB; 1.
DR SUPFAM; SSF69765; YgbB_synth; 1.
DR TIGRFAMs; TIGR00151; ispF; 1.
DR PROSITE; PS01350; ISPF; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isoprene biosynthesis; Lyase; Metal-binding.
FT CHAIN 1 158 2-C-methyl-D-erythritol 2,4-
FT cyclodiphosphate synthase.
FT /FTId=PRO_1000117417.
FT REGION 9 11 Substrate binding (By similarity).
FT REGION 35 36 Substrate binding (By similarity).
FT REGION 39 47 Substrate binding (By similarity).
FT REGION 57 59 Substrate binding (By similarity).
FT REGION 62 66 Substrate binding (By similarity).
FT REGION 101 107 Substrate binding (By similarity).
FT REGION 132 136 Substrate binding (By similarity).
FT METAL 9 9 Divalent metal cation (By similarity).
FT METAL 11 11 Divalent metal cation (By similarity).
FT METAL 43 43 Divalent metal cation (By similarity).
FT BINDING 66 66 Substrate; via carbonyl oxygen (By
FT similarity).
FT BINDING 140 140 Substrate; via carbonyl oxygen (By
FT similarity).
FT BINDING 143 143 Substrate (By similarity).
FT SITE 35 35 Transition state stabilizer (By
FT similarity).
FT SITE 134 134 Transition state stabilizer (By
FT similarity).
SQ SEQUENCE 158 AA; 17219 MW; CC61E2EB140C4824 CRC64;
MFRIGQGFDV HEFAEGRPLI IGGITIPHEK GLIGHSDADV LLHTIADACL GAIAAGDIGK
HFPDTDPAFK DADSAVLLQK VWEFVREQGY ELGNLDCTII AQKPKMAPHI ESMRKRISEL
LETSIDNINV KATTTEKLGF TGREEGIASQ AVVLLQKK
//
