ID SAHH_METC4 Reviewed; 468 AA.
AC B7KSJ4;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 01-MAY-2013, entry version 30.
DE RecName: Full=Adenosylhomocysteinase;
DE EC=3.3.1.1;
DE AltName: Full=S-adenosyl-L-homocysteine hydrolase;
DE Short=AdoHcyase;
GN Name=ahcY; OrderedLocusNames=Mchl_4897;
OS Methylobacterium extorquens (strain CM4 / NCIMB 13688)
OS (Methylobacterium chloromethanicum).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Methylobacteriaceae; Methylobacterium.
OX NCBI_TaxID=440085;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CM4 / NCIMB 13688;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T.,
RA Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Marx C., Richardson P.;
RT "Complete sequence of chromosome of Methylobacterium chloromethanicum
RT CM4.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May play a key role in the regulation of the
CC intracellular concentration of adenosylhomocysteine (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-homocysteine + H(2)O = L-
CC homocysteine + adenosine.
CC -!- COFACTOR: Binds 1 NAD per subunit (By similarity).
CC -!- PATHWAY: Amino-acid biosynthesis; L-homocysteine biosynthesis; L-
CC homocysteine from S-adenosyl-L-homocysteine: step 1/1.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the adenosylhomocysteinase family.
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DR EMBL; CP001298; ACK85662.1; -; Genomic_DNA.
DR RefSeq; YP_002423590.1; NC_011757.1.
DR ProteinModelPortal; B7KSJ4; -.
DR SMR; B7KSJ4; 6-468.
DR STRING; 440085.Mchl_4897; -.
DR EnsemblBacteria; ACK85662; ACK85662; Mchl_4897.
DR GeneID; 7114318; -.
DR KEGG; mch:Mchl_4897; -.
DR PATRIC; 22504084; VBIMetChl132133_4853.
DR eggNOG; COG0499; -.
DR HOGENOM; HOG000227986; -.
DR KO; K01251; -.
DR OMA; SAQVWVT; -.
DR ProtClustDB; PRK05476; -.
DR UniPathway; UPA00314; UER00076.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004013; F:adenosylhomocysteinase activity; IEA:HAMAP.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:HAMAP.
DR Gene3D; 3.40.50.720; -; 1.
DR HAMAP; MF_00563; AdoHcyase; 1; -.
DR InterPro; IPR000043; Adenosylhomocysteinase.
DR InterPro; IPR015878; Ado_hCys_hydrolase_NAD-bd.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR020082; S-Ado-L-homoCys_hydrolase_CS.
DR PANTHER; PTHR23420; PTHR23420; 1.
DR Pfam; PF05221; AdoHcyase; 1.
DR Pfam; PF00670; AdoHcyase_NAD; 1.
DR PIRSF; PIRSF001109; Ad_hcy_hydrolase; 1.
DR SMART; SM00996; AdoHcyase; 1.
DR SMART; SM00997; AdoHcyase_NAD; 1.
DR TIGRFAMs; TIGR00936; ahcY; 1.
DR PROSITE; PS00738; ADOHCYASE_1; 1.
DR PROSITE; PS00739; ADOHCYASE_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Hydrolase; NAD; One-carbon metabolism.
FT CHAIN 1 468 Adenosylhomocysteinase.
FT /FTId=PRO_1000196670.
FT NP_BIND 195 197 NAD (By similarity).
FT NP_BIND 258 263 NAD (By similarity).
FT NP_BIND 337 339 NAD (By similarity).
FT BINDING 57 57 Substrate (By similarity).
FT BINDING 132 132 Substrate (By similarity).
FT BINDING 194 194 Substrate (By similarity).
FT BINDING 224 224 Substrate (By similarity).
FT BINDING 228 228 Substrate (By similarity).
FT BINDING 229 229 NAD (By similarity).
FT BINDING 281 281 NAD (By similarity).
FT BINDING 316 316 NAD (By similarity).
FT BINDING 382 382 NAD (By similarity).
SQ SEQUENCE 468 AA; 51024 MW; EC7463AF4D3425D0 CRC64;
MAVANDYIVK DIGLADYGRK EISIAETEMP GLMSTRAEYG ASQPLKGAKI AGSLHMTIQT
AVLIETLKAL GADIRWVSCN IYSTQDHAAA AIAAAGIPVF AVKGETLTEY WDYTSKLFDW
HDGGMPNMIL DDGGDATMFV HLGLRAENGD TAFLDKPESE EEEVFFALLK KKLAEKPKGW
FAGLADSIKG VSEETTTGVH RLYNLAKEGK LLFPAINVND SVTKSKFDNL YGCKESLVDG
IRRGTDVMMA GKVAMVAGFG DVGKGSAASL RNAGCRVLVS EIDPICALQA AMEGYEVVTM
EDAAPRADIF VTATGNKDII TIEHMRAMKD RAIVCNIGHF DNEIQVAGLK NLKWQNIKPQ
VDEIEFADGH RIILLSEGRL VNLGNATGHP SFVMSASFTN QTLAQIELWT NPGKYERQVY
TLPKALDEKV AALHLEKIGV KLSKLRPDQA AYIGVSQTGP FKPEHYRY
//
