ID B7L2X7_METC4 Unreviewed; 527 AA.
AC B7L2X7;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=Mchl_5430 {ECO:0000313|EMBL:ACK86185.1};
OS Methylorubrum extorquens (strain CM4 / NCIMB 13688) (Methylobacterium
OS extorquens).
OG Plasmid pCMU01 {ECO:0000313|EMBL:ACK86185.1,
OG ECO:0000313|Proteomes:UP000002385}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylorubrum.
OX NCBI_TaxID=440085 {ECO:0000313|EMBL:ACK86185.1, ECO:0000313|Proteomes:UP000002385};
RN [1] {ECO:0000313|EMBL:ACK86185.1, ECO:0000313|Proteomes:UP000002385}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CM4 / NCIMB 13688 {ECO:0000313|Proteomes:UP000002385};
RC PLASMID=pCMU01 {ECO:0000313|EMBL:ACK86185.1,
RC ECO:0000313|Proteomes:UP000002385};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA Marx C., Richardson P.;
RT "Complete sequence of plasmid1 of Methylobacterium chloromethanicum CM4.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ACK86185.1, ECO:0000313|Proteomes:UP000002385}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CM4 / NCIMB 13688 {ECO:0000313|Proteomes:UP000002385};
RC PLASMID=pCMU01 {ECO:0000313|EMBL:ACK86185.1,
RC ECO:0000313|Proteomes:UP000002385};
RX PubMed=22887658; DOI=10.1128/JB.01009-12;
RA Marx C.J., Bringel F., Chistoserdova L., Moulin L., Farhan Ul Haque M.,
RA Fleischman D.E., Gruffaz C., Jourand P., Knief C., Lee M.C., Muller E.E.,
RA Nadalig T., Peyraud R., Roselli S., Russ L., Goodwin L.A., Ivanova N.,
RA Kyrpides N., Lajus A., Land M.L., Medigue C., Mikhailova N., Nolan M.,
RA Woyke T., Stolyar S., Vorholt J.A., Vuilleumier S.;
RT "Complete genome sequences of six strains of the genus Methylobacterium.";
RL J. Bacteriol. 194:4746-4748(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP001299; ACK86185.1; -; Genomic_DNA.
DR RefSeq; WP_012606088.1; NC_011758.1.
DR AlphaFoldDB; B7L2X7; -.
DR REBASE; 19569; M.MchCM4ORF5430P.
DR KEGG; mch:Mchl_5430; -.
DR HOGENOM; CLU_020255_1_0_5; -.
DR Proteomes; UP000002385; Plasmid pCMU01.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0006306; P:DNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ACK86185.1}; Plasmid {ECO:0000313|EMBL:ACK86185.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ACK86185.1}.
FT DOMAIN 43..97
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 527 AA; 58560 MW; 99584DAD9FB7585E CRC64;
MDGEGCRVTL WRKRGPWYCQ GMDSFPPTHD LVDMAEFGEA DEFASRLSRK EAGAYFTPDA
VAATLVRWAV RDRNDRLLDP SCGDGSFVAA HARSVGVERD AASAGLARER APAGEVHVGD
FFAWAERTGE RFHCAAGNPP FIRYQRFSGE TRERALRICR DLGAAFSGLT SSWAPFLVAT
AGLLRPGGRM AFVVPAEIGH APYAAPLLDF AVARFDTVHL VAIREKLFPD LSEDCWLLFA
DGYGGRTREI RLTVLDRFAP SDEPPRLFER IEVDGWRDAW RRRLRPFLMP RAAREVYRRV
ADAGEALRLG EVASVGIGYV SGANDFFHLR PSEARRLGIP KALLHPSVRN GRALTGPVLS
RADVDRWLAA DDPVLLLRLP KEGSLPPSVV AYLDGEAGRL ARTAYKCRVR SPWHAVPDVQ
VPDYLLSYMS GQRPSLVRND ARCTATNSVH CVRLRSPELR PRLEAWGSPF VELSCEIEGH
PLGGGLLKLE PREATQVVLP APGQVRAEED VLREATAVMR RWRHRAD
//
