GenomeNet

Database: UniProt
Entry: B7MEG9
LinkDB: B7MEG9
Original site: B7MEG9 
ID   SYM_ECO45               Reviewed;         677 AA.
AC   B7MEG9;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   26-NOV-2014, entry version 45.
DE   RecName: Full=Methionine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00098};
DE            EC=6.1.1.10 {ECO:0000255|HAMAP-Rule:MF_00098};
DE   AltName: Full=Methionyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00098};
DE            Short=MetRS {ECO:0000255|HAMAP-Rule:MF_00098};
GN   Name=metG {ECO:0000255|HAMAP-Rule:MF_00098};
GN   OrderedLocusNames=ECS88_2256;
OS   Escherichia coli O45:K1 (strain S88 / ExPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585035;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S88 / ExPEC;
RX   PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA   Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA   Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A.,
RA   Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M.,
RA   Dossat C., Karoui M.E., Frapy E., Garry L., Ghigo J.M., Gilles A.M.,
RA   Johnson J., Le Bouguenec C., Lescat M., Mangenot S.,
RA   Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A.,
RA   Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B.,
RA   Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT   "Organised genome dynamics in the Escherichia coli species results in
RT   highly diverse adaptive paths.";
RL   PLoS Genet. 5:E1000344-E1000344(2009).
CC   -!- FUNCTION: Is required not only for elongation of protein synthesis
CC       but also for the initiation of all mRNA translation through
CC       initiator tRNA(fMet) aminoacylation. {ECO:0000255|HAMAP-
CC       Rule:MF_00098}.
CC   -!- CATALYTIC ACTIVITY: ATP + L-methionine + tRNA(Met) = AMP +
CC       diphosphate + L-methionyl-tRNA(Met). {ECO:0000255|HAMAP-
CC       Rule:MF_00098}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00098};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_00098};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00098}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00098}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family. MetG type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00098}.
CC   -!- SIMILARITY: Contains 1 tRNA-binding domain. {ECO:0000255|HAMAP-
CC       Rule:MF_00098}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CU928161; CAR03542.1; -; Genomic_DNA.
DR   RefSeq; YP_002391946.1; NC_011742.1.
DR   ProteinModelPortal; B7MEG9; -.
DR   SMR; B7MEG9; 5-551, 570-676.
DR   STRING; 585035.ECS88_2256; -.
DR   EnsemblBacteria; CAR03542; CAR03542; ECS88_2256.
DR   GeneID; 7129612; -.
DR   KEGG; ecz:ECS88_2256; -.
DR   PATRIC; 18412224; VBIEscCol91599_2236.
DR   eggNOG; COG0143; -.
DR   HOGENOM; HOG000200400; -.
DR   KO; K01874; -.
DR   OMA; NQCIYVC; -.
DR   OrthoDB; EOG6CVV9B; -.
DR   BioCyc; ECOL585035:GJWP-2253-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:UniProtKB-HAMAP.
DR   Gene3D; 1.10.730.10; -; 1.
DR   Gene3D; 2.20.28.20; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00098; Met_tRNA_synth_type1; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR004495; Met-tRNA-synth_Ia_bsu_C.
DR   InterPro; IPR023458; Met-tRNA_ligase_1.
DR   InterPro; IPR014758; Met-tRNA_synth.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR029038; MetRS_Zn.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR002547; tRNA-bd_dom.
DR   InterPro; IPR009080; tRNAsynth_1a_anticodon-bd.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   Pfam; PF01588; tRNA_bind; 1.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF57770; SSF57770; 1.
DR   TIGRFAMs; TIGR00398; metG; 1.
DR   TIGRFAMs; TIGR00399; metG_C_term; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
DR   PROSITE; PS50886; TRBD; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis;
KW   RNA-binding; tRNA-binding; Zinc.
FT   CHAIN         1    677       Methionine--tRNA ligase.
FT                                /FTId=PRO_1000199287.
FT   DOMAIN      575    677       tRNA-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00098}.
FT   MOTIF        15     25       "HIGH" region.
FT   MOTIF       333    337       "KMSKS" region.
FT   METAL       146    146       Zinc. {ECO:0000255|HAMAP-Rule:MF_00098}.
FT   METAL       149    149       Zinc. {ECO:0000255|HAMAP-Rule:MF_00098}.
FT   METAL       159    159       Zinc. {ECO:0000255|HAMAP-Rule:MF_00098}.
FT   METAL       162    162       Zinc. {ECO:0000255|HAMAP-Rule:MF_00098}.
FT   BINDING     336    336       ATP. {ECO:0000255|HAMAP-Rule:MF_00098}.
SQ   SEQUENCE   677 AA;  76284 MW;  3528021F3AD9600B CRC64;
     MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC ADDAHGTPIM
     LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS EENRQLSELI YSRLKENGFI
     KNRTISQLYD PEKGMFLPDR FVKGTCPKCK SPDQYGDNCE VCGATYSPTE LIEPKSVVSG
     ATPVMRDSEH FFFDLPSFSE MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG
     FEIPNAPGKY FYVWLDAPIG YMGSFKNLCD KRGDSVSFDK YWKKDSTAEL YHFIGKDIVY
     FHSLFWPAML EGSNFRKPTN LFVHGYVTVN GAKMSKSRGT FIKASTWLNH FDADSLRYYY
     TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF INKRFDGVLA SELADPQLYK
     TFTDAAEVIG EAWESREFGK AIREIMALAD LANRYVDEQA PWVVAKQEGR DADLQAICSM
     GINLFRVLMT YLKPVLPKLT ERAEAFLNTE LTWDGIQQPL LGHKVNPFKA LYNRIDMKQV
     EALVEASKEE VKATAAPVTG PLADDPIQET ITFDDFAKVD LRVALIENAE FVEGSDKLLR
     LTLDLGGEKR NVFSGIRSAY PDPQALIGRH TIMVANLAPR KMRFGISEGM VMAAGPGGKD
     IFLLSPDAGA KPGHQVK
//
DBGET integrated database retrieval system