ID B7NWK6_9INFA Unreviewed; 745 AA.
AC B7NWK6;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 24-JAN-2024, entry version 59.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:ACJ26184.1};
OS Influenza A virus (A/scaly-breasted munia/Hong Kong/2433/2007(H5N1)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=573961 {ECO:0000313|EMBL:ACJ26184.1};
RN [1] {ECO:0000313|EMBL:ACJ26184.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/scaly-breasted munia/Hong Kong/2433/2007
RC {ECO:0000313|EMBL:ACJ26184.1};
RX PubMed=19239752; DOI=10.3201/eid1503.081190;
RA Smith G.J., Vijaykrishna D., Ellis T.M., Dyrting K.C., Leung Y.H., Bahl J.,
RA Wong C.W., Kai H., Chow M.K., Duan L., Chan A.S., Zhang L.J., Chen H.,
RA Luk G.S., Peiris J.S., Guan Y.;
RT "Characterization of avian influenza viruses A (H5N1) from wild birds, Hong
RT Kong, 2004-2008.";
RL Emerg. Infect. Dis. 15:402-407(2009).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; CY036131; ACJ26184.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 3: Inferred from homology;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 274..471
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 38..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..53
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACJ26184.1"
SQ SEQUENCE 745 AA; 85149 MW; D5E2F39601CFEC10 CRC64;
PVQNAISTTF PYTGDPPYSH GTGTGYTMDT VNRTHQYSEK GKWTTNTETG APQLNPIDGP
LPEDNEPSGY AQTDCVLEAM AFLEESHPGI FENSCLETME IVQQTRVDKL TQGRQTYDWT
LNRNQPAATA LANTIEIFRS NGLTANESGR LIDFLKDVME SMDKKEMEIT THFQRKRRVR
DNMTKKMVTQ RTIGKKKQRL NKRSYLIRAL TLNTMTKDAE RGKLKRRAIA TPGMQIRGFV
YFVETLARSI CEKLEQSGLP VGGNEKKAKL ANVVRKMMTN SQDTELSFTI TGDNTKWNEN
QNPRMFLAMI TYITRNQPEW FRNVLSIAPI MFSNKMARLG KGYMFESKSM KLRTQIPAEM
LANIDLKYFN ELTKKKIEKI RPLLIDGTAS LSPGMMMGMF NMLSTVLGVS ILNLGQKRYT
KTTYWWDGLQ SSDDFALIVN APNHEGIQAG VDRFYRTCKL VGINMSKKKS YINRTGTFEF
TSFFYRYGFV ANFSMELPSF GVSGINESAD MSIGVTVIKN NMINNDLGPA TAQMALQLFI
KDYRYTYRCH RGDTQIQTRR SFELKKLWEQ TRSKAGLLVS DGGPNLYNIR NLHIPEVCLK
WELMDEDYQG RLCNPLNPFV SHKEIESVNN AIVMPAHGPA KSMEYDAVAT THSWIPKRNR
SILNTSQRGI LEDEQMYQKC CNLFEKFFPS SSYRRPVGIS SMVEAMVSRA RIDARIDFES
GRIKKEEFAE IMKICSTIEE LRRQK
//
