ID B7PUU2_IXOSC Unreviewed; 449 AA.
AC B7PUU2;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 27-MAR-2024, entry version 83.
DE SubName: Full=tRNA-dihydrouridine synthase, putative {ECO:0000313|EMBL:EEC10364.1, ECO:0000313|EnsemblMetazoa:ISCW007722-PA};
GN ORFNames=IscW_ISCW007722 {ECO:0000313|EMBL:EEC10364.1};
OS Ixodes scapularis (Black-legged tick) (Deer tick).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX NCBI_TaxID=6945;
RN [1] {ECO:0000313|EMBL:EEC10364.1, ECO:0000313|Proteomes:UP000001555}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Wikel {ECO:0000313|Proteomes:UP000001555}, and Wikel colony
RC {ECO:0000313|EMBL:EEC10364.1};
RG Ixodes scapularis Genome Project Consortium;
RA Caler E., Hannick L.I., Bidwell S., Joardar V., Thiagarajan M., Amedeo P.,
RA Galinsky K.J., Schobel S., Inman J., Hostetler J., Miller J., Hammond M.,
RA Megy K., Lawson D., Kodira C., Sutton G., Meyer J., Hill C.A., Birren B.,
RA Nene V., Collins F., Alarcon-Chaidez F., Wikel S., Strausberg R.;
RT "Annotation of Ixodes scapularis.";
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:ISCW007722-PA}
RP IDENTIFICATION.
RC STRAIN=wikel {ECO:0000313|EnsemblMetazoa:ISCW007722-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
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DR EMBL; ABJB010573547; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010876482; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DS795254; EEC10364.1; -; Genomic_DNA.
DR RefSeq; XP_002406800.1; XM_002406756.1.
DR AlphaFoldDB; B7PUU2; -.
DR STRING; 6945.B7PUU2; -.
DR PaxDb; 6945-B7PUU2; -.
DR EnsemblMetazoa; ISCW007722-RA; ISCW007722-PA; ISCW007722.
DR KEGG; isc:IscW_ISCW007722; -.
DR VEuPathDB; VectorBase:ISCI007722; -.
DR VEuPathDB; VectorBase:ISCP_005441; -.
DR VEuPathDB; VectorBase:ISCW007722; -.
DR HOGENOM; CLU_013299_3_0_1; -.
DR InParanoid; B7PUU2; -.
DR OMA; QPVYETT; -.
DR Proteomes; UP000001555; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0017150; F:tRNA dihydrouridine synthase activity; IBA:GO_Central.
DR GO; GO:0002943; P:tRNA dihydrouridine synthesis; IBA:GO_Central.
DR CDD; cd19871; DSRM_DUS2L; 1.
DR CDD; cd02801; DUS_like_FMN; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR035587; DUS-like_FMN-bd.
DR InterPro; IPR044463; DUS2_DSRM.
DR InterPro; IPR018517; tRNA_hU_synthase_CS.
DR PANTHER; PTHR45936; TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE; 1.
DR PANTHER; PTHR45936:SF1; TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF01207; Dus; 1.
DR SMART; SM00358; DSRM; 1.
DR SUPFAM; SSF54768; dsRNA-binding domain-like; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS01136; UPF0034; 1.
PE 1: Evidence at protein level;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Proteomics identification {ECO:0007829|PeptideAtlas:B7PUU2};
KW Reference proteome {ECO:0000313|Proteomes:UP000001555};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00266};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 368..433
FT /note="DRBM"
FT /evidence="ECO:0000259|PROSITE:PS50137"
SQ SEQUENCE 449 AA; 50545 MW; 16DCF252AE990776 CRC64;
MTSVIDYKNK VILAPMVRIG TLPMRLLALH YGADLVYSEE LIDYRLLKCQ RLENQVLGTV
DYIDDDHQIV FRTCEKERGK NILQIGTCSP ERAVQVAKLV EKDVAGIDVN MGCPKEFSLK
GGMGAALLTQ LDKVKAILTA LVQSTTLPVT CKIRVLDRLE DTLALGKLIE STGVKALGVH
GRTKEERPQH KNRNATIKAL AEHLTIPVIA NGGSSEIVDY EDIERFRVAT GATSVMLARQ
AESNCSIFSK AGRKPIDDVI VQYLHYVSAH LKYLCRLSKQ ITSIVAVRVS VRKLLQQDYQ
PKIVFRLWKM EELYSTWQKK LQAKAKELKD LSKNDSSEPT LKRCKVGNEE VWQMEAKFVR
NMFEMSNLPK TTLINWTRKN NYPHPSYKTE AKEKSFRSVV VVDSKRYSST FLEKSKKYAE
QAAALVALYA LGLIDGSKIK GNTAGMPME
//
